Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, ELISA

Cited:

Immunohistochemistry-Paraffin, ELISA Capture

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 193208
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Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human VEGF-C
Thr103-Arg227 (Cys156Ser)
Accession # Q6FH59

Specificity

Detects human VEGF-C in direct ELISAs and Western blots. In direct ELISAs, this antibody does not cross-react with recombinant human (rh) PDGF‑AA, rhPDGF‑BB, rhPlGF, rhVEGF

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human VEGF‑C Antibody

Human VEGF‑C ELISA Standard Curve.

Recombinant Human VEGF‑C protein was serially diluted 2-fold and captured by Mouse Anti-Human VEGF‑C Monoclonal Antibody (Catalog # MAB752) coated on a Clear Polystyrene Microplate (DY990). Goat Anti-Human VEGF‑C Antigen Affinity-purified Polyclonal Antibody (AF752) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).

Applications for Human VEGF‑C Antibody

Application
Recommended Usage

ELISA

This antibody functions as an ELISA capture antibody when paired with Goat Anti-Human VEGF‑C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF752).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human VEGF-C DuoSet ELISA Kit (Catalog # DY752B) for convenient development of a sandwich ELISA or the Human VEGF-C Quantikine ELISA Kit (Catalog # DVEC00) for  a complete optimized ELISA.

Western Blot

1 µg/mL
Sample: Recombinant Human VEGF-C (Catalog # 2179-VC)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.


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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: VEGF-C

Vascular Endothelial Growth Factor C (VEGF-C) is a member of the VEGF family of secreted growth factors. The VHD form homodimers after proteolytic removal of the N- and C-terminal pro regions. VEGF-C promotes angiogenesis and lymphangiogenesis through interactions with VEGF R2 and VEGF R3.

Long Name

Vascular Endothelial Growth Factor C

Alternate Names

VEGFC

Entrez Gene IDs

7424 (Human); 22341 (Mouse)

Gene Symbol

VEGFC

UniProt

Additional VEGF-C Products

Product Documents for Human VEGF‑C Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human VEGF‑C Antibody

For research use only

Citations for Human VEGF‑C Antibody

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