Human VEGF-C Antibody

Catalog # Availability Size / Price Qty
AF752
AF752-SP
Detection of Human VEGF‑C by Western Blot.
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Product Details
Citations (10)
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Human VEGF-C Antibody Summary

Species Reactivity
Human
Specificity
Detects human VEGF-C in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) VEGF-D and rhVEGF-A is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human VEGF-C
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human VEGF‑C by Western Blot. View Larger

Detection of Human VEGF‑C by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human VEGF‑C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF752) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for VEGF‑C at approximately 52 kDa, 34 kDa, and 13 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry VEGF-C in Human Colon Cancer Tissue. View Larger

VEGF-C in Human Colon Cancer Tissue. VEGF-C was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Goat Anti-Human VEGF-C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF752) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue).Specific labeling was localized to stromal cells surrounding crypts in the colon mucosa (cross section across crypts). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry VEGF-C in Human Colon Cancer Tissue. View Larger

VEGF-C in Human Colon Cancer Tissue. VEGF-C was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Goat Anti-Human VEGF-C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF752) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to epithelial cells in crypts of the colon mucusa (longitudinal section of crypts). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VEGF-C

Vascular endothelial growth factor C (VEGF-C) and VEGF-D constitute a VEGF sub-family that share the conserved VEGF homology domain (VHD) with other VEGF family members but are distinguished by their preferential formation of non-covalent homodimers. Both VEGF-C and -D have long N- and C-terminal propeptide extensions. The VEGF-C propeptide undergoes stepwise proteolytic processing to generate ligands with increasing affinity for VEGF-R3. However, only the fully processed VEGF-C containing just the VHD can bind VEGF-R2. None of the VEGF-C forms have appreciable affinity for VEGF-R1. VEGF-C is expressed in multiple adult human tissues, most prominently in lymph nodes, heart, placenta, ovary, and small intestine. Traces of VEGF-C are also detected in brain, liver, thymus, skeletal muscles, spleen, prostate, testis and colon. Unlike other VEGF family members, VEGF-C expression is not regulated by hypoxia. VEGF-C is a lymphangiogenic growth factor and the VEGF-C/VEGF-R3 signaling pathway has been shown to be crucial for lymphangiogenesis. VEGF-C and VEGF-R3 are usually co-expressed at sites with lymphatic vessel sprouting, in the embryo, and in various pathological conditions. VEGF-C stimulates lymphangiogenesis in the avian chorioallantoic membrane model. Over-expression of VEGF-C in breast cancer cells has been shown to increase intratumoral lymphangiogenesis, resulting in enhanced metastasis to regional lymph nodes and to the lungs. Mouse tumors expressing elevated levels of VEGF-C have increased lymphatic metastasis and increased lymphatic surface area in the tumor margin. VEGF-C is also associated with lymph node metastasis of colorectal carcinoma. Besides lymphangiogenesis, VEGF-C can have potent effects on physiological angiogenesis through its interaction with VEGF R2. The protein can stimulate migration and proliferation of endothelial cells in vitro and in vivo and has been shown to stimulate angiogenesis in the mouse cornea and in rabbit hind limb ischaemia.

Long Name
Vascular Endothelial Growth Factor C
Entrez Gene IDs
7424 (Human); 22341 (Mouse)
Alternate Names
Flt4 ligand; Flt4-L; vascular endothelial growth factor C; Vascular endothelial growth factor-related protein; VEGFC; VEGF-C; VRPFLT4 ligand DHM

Product Datasheets

Citations for Human VEGF-C Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Synaptonemal complex protein 3 is associated with lymphangiogenesis in non-small cell lung cancer patients with lymph node metastasis
    Authors: H Kitano, JY Chung, KH Noh, YH Lee, TW Kim, SH Lee, SH Eo, HJ Cho, CH Choi, S Inoue, J Hanaoka, J Fukuoka, SM Hewitt
    J Transl Med, 2017;15(1):138.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Efficient activation of the lymphangiogenic growth factor VEGF-C requires the C-terminal domain of VEGF-C and the N-terminal domain of CCBE1
    Authors: SK Jha, K Rauniyar, T Karpanen, VM Leppänen, P Brouillard, M Vikkula, K Alitalo, M Jeltsch
    Sci Rep, 2017;7(1):4916.
    Species: Primate - Chlorocebus aethiops (African Green Monkey)
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. The interplay of matrix metalloproteinase-8, transforming growth factor-?1 and vascular endothelial growth factor-C cooperatively contributes to the aggressiveness of oral tongue squamous cell carcinoma
    Authors: P Åström, K Juurikka, ES Hadler-Ols, G Svineng, NK Cervigne, RD Coletta, J Risteli, JH Kauppila, S Skarp, S Kuttner, A Oteiza, M Sutinen, T Salo
    Br. J. Cancer, 2017;117(7):1007-1016.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  4. A phase Ib/II translational study of sunitinib with neoadjuvant radiotherapy in soft-tissue sarcoma.
    Authors: Lewin J, Khamly K, Young R, Mitchell C, Hicks R, Toner G, Ngan S, Chander S, Powell G, Herschtal A, Te Marvelde L, Desai J, Choong P, Stacker S, Achen M, Ferris N, Fox S, Slavin J, Thomas D
    Br J Cancer, 2014;111(12):2254-61.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  5. Dilated thin-walled blood and lymphatic vessels in human endometrium: a potential role for VEGF-D in progestin-induced break-through bleeding.
    Authors: Donoghue JF, McGavigan CJ, Lederman FL
    PLoS ONE, 2012;7(2):e30916.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Lysophosphatidic Acid Enhances Vascular Endothelial Growth Factor-C Expression in Human Prostate Cancer PC-3 Cells.
    Authors: Lin CE, Chen SU, Lin CC
    PLoS ONE, 2012;7(7):e41096.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Autocrine loop between vascular endothelial growth factor (VEGF)-C and VEGF receptor-3 positively regulates tumor-associated lymphangiogenesis in oral squamoid cancer cells.
    Authors: Matsuura M, Onimaru M, Yonemitsu Y, Suzuki H, Nakano T, Ishibashi H, Shirasuna K, Sueishi K
    Am. J. Pathol., 2009;175(4):1709-21.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  8. Lymphatic reprogramming of microvascular endothelial cells by CEA-related cell adhesion molecule-1 via interaction with VEGFR-3 and Prox1.
    Authors: Kilic N, Oliveira-Ferrer L, Neshat-Vahid S, Irmak S, Obst-Pernberg K, Wurmbach JH, Loges S, Kilic E, Weil J, Lauke H, Tilki D, Singer BB, Ergun S
    Blood, 2007;110(13):4223-33.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. VEGF-C is a trophic factor for neural progenitors in the vertebrate embryonic brain.
    Authors: Le Bras B, Barallobre MJ, Homman-Ludiye J, Ny A, Wyns S, Tammela T, Haiko P, Karkkainen MJ, Yuan L, Muriel MP, Chatzopoulou E, Breant C, Zalc B, Carmeliet P, Alitalo K, Eichmann A, Thomas JL
    Nat. Neurosci., 2006;9(3):340-8.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  10. Cyclooxygenase-2 induces EP1- and HER-2/Neu-dependent vascular endothelial growth factor-C up-regulation: a novel mechanism of lymphangiogenesis in lung adenocarcinoma.
    Authors: Su JL, Shih JY, Yen ML, Jeng YM, Chang CC, Hsieh CY, Wei LH, Yang PC, Kuo ML
    Cancer Res., 2004;64(2):554-64.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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