Human Vimentin Antibody

(13 citations)
(2 Reviews)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human Vimentin in Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human Vimentin
    Ser2-Glu466
    Accession # P08670
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Simple Western
    10 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Vimentin by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Vimentin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2105) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Vimentin at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
Vimentin in Human Skin. Vimentin was detected in immersion fixed paraffin-embedded sections of human skin using 10 µg/mL Goat Anti-Human Vimentin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2105) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Immunocytochemistry
Vimentin in HeLa Human Cell Line. Vimentin was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human Vimentin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2105) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to intermediate filaments in cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Human Vimentin by Simple WesternTM. Simple Western lane view shows lysates of K562 human chronic myelogenous leukemia cell line and Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for Vimentin at approximately 59 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Vimentin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2105) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Vimentin

Vimentin is a 57 kDa class III intermediate filament (IF) protein that belongs to the intermediate filament family. It is the predominant IF in cells of mesenchymal origin such as vascular endothelium and blood cells (1‑3). The human Vimentin cDNA encodes a 466 amino acid (aa) protein that contains head and tail regions with multiple regulatory Ser/Thr phosphorylation sites, and a central rod domain with three coiled-coil regions separated by linkers (1, 2). Human Vimentin shares 97‑98% aa identity with mouse, rat, ovine, bovine and canine Vimentin. Sixteen Vimentin coiled-coil dimers self-assemble to form intermediate (10‑12 nm wide) filaments (4). These filaments then anneal longitudinally to form non-polarized fibers that support cell structure and withstand stress (4). IF fibers are highly dynamic, and half-life depends on the balance between kinase and phosphatase activity. For example, phosphorylation followed by dephosphorylation drives IF disintegration, followed by reorganization during mitosis (1, 5, 6). Interactions of head and tail domains link IFs with other structures such as actin and microtubule cytoskeletons (7). Vimentin is involved in positioning autophagosomes, lysosomes and the Golgi complex within the cell (8). It facilitates cell migration and motility by recycling internalized trailing edge integrins back to the cell surface at the leading edge (9‑11). Vimentin helps maintain the lipid composition of cellular membranes, and caspase cleavage of Vimentin is a key event in apoptosis (8, 12). Phosphorylation promotes secretion of Vimentin by TNF-alpha -stimulated macrophages (13). Extracellular Vimentin has been shown to associate with several microbes, and appears to promote an antimicrobial oxidative burst (13, 14). Cell-associated Vimentin can also interact with NKp46 to recruit NK cells to tuberculosis-infected monocytes (15).

  • References:
    1. Omary, M.B. et al. (2006) Trends Biochem. Sci. 31:383.
    2. Ivaska, J. et al. (2007) Exp. Cell Res. 313:2050.
    3. Ferrari, S. et al. (1986) Mol. Cell. Biol. 6:3614.
    4. Sokolova, A.V. et al. (2006) Proc. Natl. Acad. Sci. USA 103:16206.
    5. Eriksson, J.E. et al. (2004) J. Cell Sci. 117:919.
    6. Li, Q.-F. et al. (2006) J. Biol. Chem. 281:34716.
    7. Esue, O. et al. (2006) J. Biol. Chem. 281:30393.
    8. Styers, M.L. et al. (2005) Traffic 6:359.
    9. McInroy, L. and A. Maata (2007) Biochem. Biophys. Res. Commun. 360:109.
    10. Nieminen, M. et al. (2006) Nat. Cell Biol. 8:156.
    11. Ivaska, J. et al. (2005) EMBO J. 24:3834.
    12. Byun, Y. et al. (2001) Cell Death Differ. 8:443.
    13. Mor-Vaknin, N. et al. (2003) Nat. Cell Biol. 5:59.
    14. Zou, Y. et al. (2006) Biochem. Biophys. Res. Commun. 351:625.
    15. Garg, A. et al. (2006) J. Immunol. 177:6192.
  • Entrez Gene IDs:
    7431 (Human); 22352 (Mouse); 81818 (Rat)
  • Alternate Names:
    FLJ36605; VIM; Vimentin
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Acute cigarette smoke exposure activates apoptotic and inflammatory programs but a second stimulus is required to induce epithelial to mesenchymal transition in COPD epithelium
    Authors: LA Murray, R Dunmore, A Camelo, CA Da Silva, MJ Gustavsson, DM Habiel, TL Hackett, CM Hogaboam, MA Sleeman, DA Knight
    Respir. Res., 2017;18(1):82.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC paraffin embedded
  2. Differentiation of Human Induced-Pluripotent Stem Cells into Smooth-Muscle Cells: Two Novel Protocols.
    Authors: Yang L, Geng Z, Nickel T, Johnson C, Gao L, Dutton J, Hou C, Zhang J
    PLoS ONE, 2016;11(1):e0147155.
    Species: Human
    Sample Type: Whole Cells
    Application: IHC Not Specified
  3. TGF-?1 Is Present at High Levels in Wound Fluid from Breast Cancer Patients Immediately Post-Surgery, and Is Not Increased by Intraoperative Radiation Therapy (IORT)
    PLoS ONE, 2016;11(9):e0162221.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  4. The mosaic of "seronegative" antiphospholipid syndrome.
    Authors: Conti F, Capozzi A, Truglia S, Lococo E, Longo A, Misasi R, Alessandri C, Valesini G, Sorice M
    J Immunol Res, 2014;2014(0):389601.
    Species: Human
    Sample Type: Serum
    Application: ELISA Development
  5. Cellular vimentin regulates construction of dengue virus replication complexes through interaction with NS4A protein.
    Authors: Teo, Catherin, Chu, Justin J
    J Virol, 2014;88(4):1897-913.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  6. In vivo biomarker expression patterns are preserved in 3D cultures of Prostate Cancer.
    Authors: Windus L, Kiss D, Glover T, Avery V
    Exp Cell Res, 2012;318(19):2507-19.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  7. Fibroblast activation protein regulates tumor-associated fibroblasts and epithelial ovarian cancer cells.
    Authors: Lai D, Ma L, Wang F
    Int. J. Oncol., 2012;41(2):541-50.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  8. Angiotensin II Contributes to Renal Fibrosis Independently of Notch Pathway Activation.
    Authors: Lavoz C, Rodrigues-Diez R, Benito-Martin A, Rayego-Mateos S, Rodrigues-Diez RR, Alique M, Ortiz A, Mezzano S, Egido J, Ruiz-Ortega M
    PLoS ONE, 2012;7(7):e40490.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  9. Vimentin/cardiolipin complex as a new antigenic target of the antiphospholipid syndrome.
    Authors: Ortona E, Capozzi A, Colasanti T, Conti F, Alessandri C, Longo A, Garofalo T, Margutti P, Misasi R, Khamashta MA, Hughes GR, Valesini G, Sorice M
    Blood, 2010;116(16):2960-7.
    Species: Human
    Sample Type: Recombinant Protein
    Application: ELISA Development
  10. Glycogene expression alterations associated with pancreatic cancer epithelial-mesenchymal transition in complementary model systems.
    Authors: Maupin KA, Sinha A, Eugster E, Miller J, Ross J, Paulino V, Keshamouni VG, Tran N, Berens M, Webb C, Haab BB
    PLoS ONE, 2010;5(9):e13002.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  11. Multipotent mesenchymal progenitor cells are present in endarterectomized tissues from patients with chronic thromboembolic pulmonary hypertension.
    Authors: Firth AL, Yao W, Ogawa A, Madani MM, Lin GY, Yuan JX
    Am. J. Physiol., Cell Physiol., 2010;298(5):C1217-25.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  12. Morphogenesis and biological significance of spindle cell transformation in a spindle cell carcinoma.
    Authors: Kim EJ, Che ZM, Park YJ, Hwang YS, Kim KY, Jung da W, Jeon NK, Choi YW, Lee EJ, Kim J
    Cancer Lett., 2009;275(1):61-71.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  13. Induction of epithelial-mesenchymal transition in primary airway epithelial cells from patients with asthma by transforming growth factor-beta1.
    Authors: Hackett TL, Warner SM, Stefanowicz D, Shaheen F, Pechkovsky DV, Murray LA, Argentieri R, Kicic A, Stick SM, Bai TR, Knight DA
    Am. J. Respir. Crit. Care Med., 2009;180(2):122-33.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
Expand to show all 13 Citations
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Average Rating: 4 (Based on 2 reviews)

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We have 2 reviews tested in 2 species: Human, Mouse.
We have 2 review tested in 1 application: Immunocytochemistry/Immunofluorescence.

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Images Ratings Applications Species Reviewed By Date Details
Immunocytochemistry/Immunofluorescence Human Vimentin Antibody AF2105
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 ICC/IF Human Anonymous 12/10/2017
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Immunocytochemistry/Immunofluorescence Human Vimentin Antibody AF2105
Immunocytochemistry/Immunofluorescence: Human Vimentin Antibody [AF2105]

Summary

ApplicationImmunocytochemistry/Immunofluorescence
Sample TestedKidney cancer tissue
SpeciesHuman
Immunocytochemistry/Immunofluorescence Human Vimentin Antibody AF2105
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 ICC/IF Mouse Anonymous 07/22/2016
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Immunocytochemistry/Immunofluorescence Human Vimentin Antibody AF2105
Immunocytochemistry/Immunofluorescence: Human Vimentin Antibody [AF2105]

Summary

ApplicationImmunocytochemistry/Immunofluorescence
Sample TestedCerebellum tissue
SpeciesMouse

Other Experimental Details

Other Experimental DetailsP7 MOUSE CEREBELLUM. Radial Glia

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