Human Wnt-10b Antibody

    
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  • Species Reactivity
    Human
  • Specificity
    Detects human Wnt-10b in direct ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant mouse Wnt-10b is observed and no cross-reactivity with recombinant human Wnt-6 is observed.
  • Source
    Monoclonal Mouse IgG2A Clone # 793116
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant human Wnt-10b
    Asn29-Lys389
    Accession # O00744
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunohistochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Immunohistochemistry
Wnt‑10b in Human Breast Cancer Tissue. Wnt‑10b was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human Wnt‑10b Monoclonal Antibody (Catalog # MAB71961) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter­stained with hematoxylin (blue). Specific staining was localized to cytoplasm of cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Wnt-10b

Wnt-10b (also known as Wnt-12) is a 42‑44 kDa member of the Wnt family of secreted, highly conserved, cysteine-rich glycoproteins that play important roles in vertebrate pattern formation, cell fate decision, axon guidance, and tumor formation (1‑3). Human Wnt-10b cDNA encodes a 389 amino acid (aa) precursor that contains a 28 aa signal sequence plus a 361 aa mature protein that contains two glycosylation sites, three potential phosphorylation sites, and a potential palmitoylation site (3, 4). Human Wnt-10b shares 97‑99% aa identity with mouse, rat, equine, porcine, and canine Wnt-10b. Wnt-10b plays a critical role in maintaining mesenchymal stem cells and determining whether they differentiate to adipocytes or osteoblasts (5‑7). Mouse Wnt-10b deletion produces age‑dependent loss of bone mass due to defective production of osteoblasts, while transgenic over‑expression increases postnatal osteoblast differentiation and inhibits adipocyte differentiation (5‑7). Ectopic expression of Wnt-10b in an obesity and diabetes‑prone background, such as the ob/ob mouse, inhibits obesity (8). In mouse skeletal muscle, Wnt-10b is expressed inversely with SREBP1c and increases insulin sensitivity (9). In humans, a missense polymorphism is responsible for a malformation of hands and feet, while a C256Y inactivating mutation is associated with severe early-onset obesity (10, 11). Wnt-10b is mainly produced by stem cells and pre-osteoblasts, but also by adult bone marrow CD8+ T lymphocytes stimulated with parathyroid hormone (12). In some hepatocellular carcinomas, Wnt-10b can inhibit cancer cell growth, but in others, it can act synergistically with FGFs to stimulate cell growth (13). Several Wnts, including Wnt-10b, are expressed in both normal and/or malignant colon tissues (14).

  • References:
    1. Hardiman, G. et al. (1997) Cytogenet. Cell Genet. 77:278.
    2. Katoh, M. (2008) Curr. Drug Targets 9:565.
    3. Hausmann, G. et al. (2007) Nat. Rev. Mol. Cell Biol. 8:331.
    4. Swiss Prot Accession # O00744.
    5. Stevens, J.R. et al. (2010) J. Bone Miner. Res. 25:2138.
    6. Bennett, C.N. et al. (2007) J. Bone Miner. Res. 22:1924.
    7. Bennett, C.N. et al. (2005) Proc. Natl. Acad. Sci. USA 102:3324.
    8. Wright, W.S. et al. (2007) Diabetes 56:295.
    9. Abiola, M. et al. (2009) PLoS ONE 4:e8509.
    10. Ugur, S.A. and A. Tolun (2008) Hum. Mol. Genet. 17:2644.
    11. Christodoulides, C. et al. (2006) Diabetologia 49:678.
    12. Terauchi, M. et al. (2009) Cell Metab. 10:229.
    13. Yoshikawa, H. et al. (2007) Mol. Biol. Cell 18:4292.
    14. Holcombe, R.F. et al. (2002) J. Clin. Pathol: Mol. Pathol. 55:220.
  • Long Name:
    Wingless-type MMTV Integration Site Family, Member 10B
  • Entrez Gene IDs:
    7480 (Human); 22410 (Mouse)
  • Alternate Names:
    protein Wnt-10b; Protein Wnt-12; SHFM6WNT-10B protein; wingless-type MMTV integration site family, member 10B; Wnt10b; Wnt-10b; WNT12; Wnt-12
Related Research Areas

FAQs

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Cell and Tissue Staining Kits
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Anti-Mouse HRP-DAB Cell & Tissue Staining Kit

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Immunohistochemistry Reagents
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Antigen Retrieval Reagent-Universal

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Antigen Retrieval Reagent-Basic

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Mounting Medium

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VisUCyte Antigen Retrieval Reagent-Acidic

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NorthernLights Guard Mounting Media

NL996
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Isotype Controls
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Mouse IgG2A Isotype Control

Ctrl MAB003 35  
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Mouse IgG2A Isotype Control

Ctrl MAB0031 7  
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Mouse IgG2A Isotype Control

Ctrl MAB003R
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Reconstitution Buffers
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Reconstitution Buffer 1 (PBS)

RB01
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Secondary Antibodies
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Mouse IgG HRP-conjugated Antibody

WB HAF007 32  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow , IHC , ICC NL007 10
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow , IHC , ICC NL008 8
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 4  
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG Antibody

WB AF007 4
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow , IHC , ICC NL009 5
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG2A Antibody

WB , Flow MAB0033  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Rat Anti-Mouse IgG2A PE-conjugated Antibody

Flow F0129 1  
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Mouse IgG2A Antibody

Flow MAB0032  
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Rat Anti-Mouse IgG2A APC-conjugated Antibody

Flow F0130  
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Rat Anti-Mouse IgG2A PerCP-conjugated Antibody

Flow F0131  
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