Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse, Transgenic Mouse

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Adiponectin/Acrp30 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Adiponectin/Acrp30
Glu18-Asn247
Accession # Q60994

Specificity

Detects mouse Adiponectin/Acrp30 in direct ELISAs and Western blots.   In direct ELISAs, greater than 50% cross-reactivity with recombinant rat Adiponectin is observed and less than 30% cross-reactivity with recombinant human Adiponectin is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse Adiponectin/Acrp30 Antibody

Adiponectin/Acrp30 antibody in ST-2 mouse bone marrow-derived stromal cell line differentiated to adipocytes by Immunocytochemistry (ICC).

Adiponectin/Acrp30 in ST-2 mouse bone marrow-derived stromal cell line differentiated to adipocytes.

Adiponectin/Acrp30 was detected in immersion fixed ST-2 mouse bone marrow-derived stromal cell line differentiated to adipocytes using Goat Anti-Mouse Adiponectin/Acrp30 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1119) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counter-stained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Adiponectin/Acrp30 antibody in human mesenchymal stem cells differentiated to adipocytes by Immunocytochemistry (ICC).

Adiponectin/Acrp30 in human mesenchymal stem cells differentiated to adipocytes.

Adiponectin/Acrp30 was detected in immersion fixed human mesenchymal stem cells differentiated to adipocytes using Goat Anti-Mouse Adiponectin/Acrp30 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1119) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Mouse Adiponectin/Acrp30 by Western Blot

Detection of Mouse Adiponectin/Acrp30 by Western Blot

Chronic administration of compound 21 increases the abundance of adiponectin and UCP‐1 in adipose tissue. (A) and (C) Abundance of adiponectin and UCP‐1 in adipose tissue as determined by western blotting. (B) and (D) Levels of adiponectin and UCP‐1 as determined by immunohistochemistry. Quantification of specific bands was performed with Gel‐Pro Analyzer software. (A) and (C) Scatter dot plots show individual values and superimposed bar graphs represent the means ± S.E. (B) and (D) Positive adiponectin and UCP‐1 staining was quantified using Image‐Pro Plus 4.5 software. Data were analyzed by unpaired two‐tailed Student's t test, where **P < 0.01 when compared with saline‐treated control mice. For adiponectin, (C21, n = 9 per group; control, n = 9 per group). For UCP‐1, (C21, n = 9 per group, control, n = 10 per group). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/30156060), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Adiponectin/Acrp30 by Western Blot

Detection of Mouse Adiponectin/Acrp30 by Western Blot

HIF-1 activation-induced T-cadherin upregulation increased adiponectin accumulation and EV production. (A) Western blot analysis of total cell lysates. UV-F2 cells cultured in DMEM containing 5% serum from adiponectin knockout mice were treated with or without high molecular weight adiponectin (HMW-APN) (10 μg/mL) and roxadustat (Roxa) (50 μM) for 48 h (n = 3 for each group). (B) Western blot analysis of EVs isolated from cell culture medium by differential ultracentrifugation. UV-F2 cells cultured in FBS-free Advanced DMEM were treated with or without HMW-APN (20 μg/mL) and roxadustat (50 μM) for 48 h (n = 3 for each group). Alix, TSG101, and syntenin were evaluated as EV markers. (C) Western blot analysis of total cell lysates. UV-F2 cells transfected control (Cont) or T-cadherin (T-cad) siRNA were cultured in DMEM containing 5% serum from adiponectin knockout mice with or without HMW-APN (20 μg/mL) and roxadustat (50 μM) for 48 h (n = 3 for each group). (D) Western blot analysis of EVs isolated from cell culture medium. UV-F2 cells transfected Cont or T-cad siRNA were cultured in FBS-free Advanced DMEM with or without HMW-APN (20 μg/mL) and roxadustat (50 μM) for 48 h (n = 3). Data are means ± SEMs. *p < 0.05, **p < 0.01, and ***p < 0.001 (Tukey–Kramer test). Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/s41598-024-51935-6), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse Adiponectin/Acrp30 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed human mesenchymal stem cells differentiated to adipocytes and immersion fixed ST-2 mouse bone marrow-derived stromal cell line differentiated to adipocytes using Human/Mouse StemXVivo Osteogenic/Adipogenic Base Media (Catalog # CCM007) and Human/Mouse StemXVivo Adipogenic Supplement (Catalog # CCM011)

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Adiponectin/Acrp30 (Catalog # 5095-AC)

Reviewed Applications

Read 2 reviews rated 4.5 using AF1119 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Adiponectin/Acrp30

Adiponectin, also known as Acrp30, is an adipocyte-derived protein with wide ranging paracrine and endocrine effects on metabolism and inflammation. It promotes adipocyte differentiation, fatty acid catabolism, and insulin sensitivity, and is negatively correlated with obesity, type 2 diabetes, and atherogenesis. In this context, adiponectin is an anti-inflammatory agent, but it exerts pro-inflammatory effects in nonmetabolic disorders such as rheumatoid arthritis and inflammatory bowel disease (1-3). Adiponectin interacts with the receptors AdipoR1 and AdipoR2, calreticulin, and Cadherin-13/T-Cadherin, as well as with several growth factors (4-7). Mature mouse adiponectin consists of a 66 amino acid (aa) N-terminal collagenous region and a 137 aa C-terminal C1q-like globular domain which can be cleaved by a leukocyte-derived elastase (8-10). Mature mouse adiponectin shares 83% and 91% amino acid (aa) sequence identity with human and rat adiponectin, respectively. Adiponectin associates into trimers that may assemble into medium molecular weight (MMW) hexamers and then into >300 kDa high molecular weight (HMW) oligomers (11-13). The glycosylation of four hydroxylated lysine residues in the collagenous domain is required for the intracellular formation of HMW complexes (14). The various multimeric forms of adiponectin exhibit distinct tissue specific and gender specific profiles and activities (13, 15).

References

  1. Lara-Castro, C. et al. (2007) Curr. Opin. Lipidol. 18:263.
  2. Tilg, H. and A.R. Moschen (2006) Nat. Rev. Immunol. 6:772.
  3. Fantuzzi, G. (2008) J. Allergy Clin. Immunol. 121:326.
  4. Yamauchi, T. et al. (2007) Nat. Med. 13:332.
  5. Takemura, Y. et al. (2007) J. Clin. Invest. 117:375.
  6. Hug, C. et al. (2004) Proc. Natl. Acad. Sci. USA 101:10308.
  7. Wang, Y. et al. (2005) J. Biol. Chem. 280:18341.
  8. Scherer, P.E. et al. (1995) J. Biol. Chem. 270:26746.
  9. Hu, E. et al. (1996) J. Biol. Chem. 271:10697.
  10. Waki, H. et al. (2005) Endocrinology 146:790.
  11. Waki, H. et al. (2003) J. Biol. Chem. 278:40352.
  12. Tsao, T.S. et al. (2003) J. Biol. Chem. 278:50810.
  13. Wang, Y. et al. (2008) Biochem. J. 409:623.
  14. Wang, H. et al. (2006) J. Biol. Chem. 281:16391.
  15. Pajvani, U.B. et al. (2003) J. Biol. Chem. 278:9073.

Alternate Names

Acrp30, AdipoQ, ApM1, GBP28

Entrez Gene IDs

9370 (Human); 11450 (Mouse); 246253 (Rat)

Gene Symbol

ADIPOQ

UniProt

Q60994 (Mouse)

Additional Adiponectin/Acrp30 Products

Product Documents for Mouse Adiponectin/Acrp30 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Adiponectin/Acrp30 Antibody

For research use only

Citations for Mouse Adiponectin/Acrp30 Antibody

Customer Reviews for Mouse Adiponectin/Acrp30 Antibody (2)

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  • Name: Anonymous
    Verified Customer | Posted 04/26/2017
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: See PMID 23824191
    Species: Mouse
    Verified Customer | Posted 01/05/2015

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