hh Mouse Adiponectin/Acrp30 Antibody AF1119: R&D Systems

Mouse Adiponectin/Acrp30 Antibody

(12 citations)
(1 Review)
  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Adiponectin/Acrp30 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant rat Adiponectin is observed and approximately 30% cross-reactivity with recombinant human Adiponectin is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse Adiponectin/Acrp30 (R&D Systems, Catalog # 5095-AC)
    Glu18-Asn247
    Accession # Q60994
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse Adiponectin/Acrp30 (Catalog # 5095-AC)
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
Adiponectin/Acrp30 in ST-2 mouse bone marrow-derived stromal cell line differentiated to adipocytes. Adiponectin/Acrp30 was detected in immersion fixed ST-2 mouse bone marrow-derived stromal cell line differentiated to adipocytes using Goat Anti-Mouse Adiponectin/Acrp30 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1119) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counter­stained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunocytochemistry
Adiponectin/Acrp30 in human mesenchymal stem cells differentiated to adipocytes. Adiponectin/Acrp30 was detected in immersion fixed human mesenchymal stem cells differentiated to adipocytes using Goat Anti-Mouse Adiponectin/Acrp30 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1119) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Adiponectin/Acrp30

Adiponectin, also known as Acrp30, is an adipocyte-derived protein with wide ranging paracrine and endocrine effects on metabolism and inflammation. It promotes adipocyte differentiation, fatty acid catabolism, and insulin sensitivity, and is negatively correlated with obesity, type 2 diabetes, and atherogenesis. In this context, adiponectin is an anti-inflammatory agent, but it exerts pro-inflammatory effects in nonmetabolic disorders such as rheumatoid arthritis and inflammatory bowel disease (1-3). Adiponectin interacts with the receptors AdipoR1 and AdipoR2, calreticulin, and Cadherin-13/T-Cadherin, as well as with several growth factors (4-7). Mature mouse adiponectin consists of a 66 amino acid (aa) N-terminal collagenous region and a 137 aa C-terminal C1q-like globular domain which can be cleaved by a leukocyte-derived elastase (8-10). Mature mouse adiponectin shares 83% and 91% amino acid (aa) sequence identity with human and rat adiponectin, respectively. Adiponectin associates into trimers that may assemble into medium molecular weight (MMW) hexamers and then into >300 kDa high molecular weight (HMW) oligomers (11-13). The glycosylation of four hydroxylated lysine residues in the collagenous domain is required for the intracellular formation of HMW complexes (14). The various multimeric forms of adiponectin exhibit distinct tissue specific and gender specific profiles and activities (13, 15).

  • References:
    1. Lara-Castro, C. et al. (2007) Curr. Opin. Lipidol. 18:263.
    2. Tilg, H. and A.R. Moschen (2006) Nat. Rev. Immunol. 6:772.
    3. Fantuzzi, G. (2008) J. Allergy Clin. Immunol. 121:326.
    4. Yamauchi, T. et al. (2007) Nat. Med. 13:332.
    5. Takemura, Y. et al. (2007) J. Clin. Invest. 117:375.
    6. Hug, C. et al. (2004) Proc. Natl. Acad. Sci. USA 101:10308.
    7. Wang, Y. et al. (2005) J. Biol. Chem. 280:18341.
    8. Scherer, P.E. et al. (1995) J. Biol. Chem. 270:26746.
    9. Hu, E. et al. (1996) J. Biol. Chem. 271:10697.
    10. Waki, H. et al. (2005) Endocrinology 146:790.
    11. Waki, H. et al. (2003) J. Biol. Chem. 278:40352.
    12. Tsao, T.S. et al. (2003) J. Biol. Chem. 278:50810.
    13. Wang, Y. et al. (2008) Biochem. J. 409:623.
    14. Wang, H. et al. (2006) J. Biol. Chem. 281:16391.
    15. Pajvani, U.B. et al. (2003) J. Biol. Chem. 278:9073.
  • Entrez Gene IDs:
    9370 (Human); 11450 (Mouse); 246253 (Rat)
  • Alternate Names:
    ACDC; Acrp30; ACRP30ADPN; adipocyte, C1Q and collagen domain containing; Adiponectin; adiponectin, C1Q and collagen domain containing; AdipoQ; ADIPQTL1; ApM1; apM-1; APM1APM-1; C1q and collagen domain-containing protein; GBP28; GBP28apM1; Gelatin-binding protein
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. The unique prodomain of T-cadherin plays a key role in adiponectin binding with the essential extracellular cadherin repeats 1 and 2
    Authors: S Fukuda, S Kita, Y Obata, Y Fujishima, H Nagao, S Masuda, Y Tanaka, H Nishizawa, T Funahashi, J Takagi, N Maeda, I Shimomura
    J. Biol. Chem, 2017;0(0):.
    Species: Mouse
    Sample Type: Serum
    Application: WB
  2. Retinol-binding protein 7 is an endothelium-specific PPAR? cofactor mediating an antioxidant response through adiponectin
    Authors: C Hu, HL Keen, KT Lu, X Liu, J Wu, DR Davis, SC Ibeawuchi, S Vogel, FW Quelle, CD Sigmund
    JCI Insight, 2017;2(6):e91738.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  3. Role of the adipose PPAR?-adiponectin axis in susceptibility to stress and depression/anxiety-related behaviors
    Mol. Psychiatry, 2016;0(0):.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  4. Positive feedback regulation between adiponectin and T-cadherin impacts adiponectin levels in tissue and plasma of male mice.
    Authors: Matsuda, Keisuke, Fujishima, Yuya, Maeda, Norikazu, Mori, Takuya, Hirata, Ayumu, Sekimoto, Ryohei, Tsushima, Yu, Masuda, Shigeki, Yamaoka, Masaya, Inoue, Kana, Nishizawa, Hitoshi, Kita, Shunbun, Ranscht, Barbara, Funahashi, Tohru, Shimomura, Iichiro
    Endocrinology, 2015;156(3):934-46.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  5. Ultrastructural localization of adiponectin protein in vasculature of normal and atherosclerotic mice.
    Authors: Mori T, Koyama Y, Maeda N, Nakamura Y, Fujishima Y, Matsuda K, Funahashi T, Shimada S, Shimomura I
    Sci Rep, 2014;4(0):4895.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC OCT-embedded
  6. Distinct fibroblast lineages determine dermal architecture in skin development and repair.
    Authors: Driskell R, Lichtenberger B, Hoste E, Kretzschmar K, Simons B, Charalambous M, Ferron S, Herault Y, Pavlovic G, Ferguson-Smith A, Watt F
    Nature, 2013;504(7479):277-81.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  7. T-cadherin is essential for adiponectin-mediated revascularization.
    Authors: Parker-Duffen, Jennifer, Nakamura, Kazuto, Silver, Marcy, Kikuchi, Ryosuke, Tigges, Ulrich, Yoshida, Sumiko, Denzel, Martin S, Ranscht, Barbara, Walsh, Kenneth
    J Biol Chem, 2013;288(34):24886-97.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: WB
  8. Leucine-rich glioma inactivated 3 associates negatively with adiponectin.
    Authors: Kim H, Kwon N, Baek K, Kim D, Yun H
    Cytokine, 2013;62(2):206-9.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  9. Adiponectin promotes monocyte-to-fibroblast transition in renal fibrosis.
    Authors: Yang J, Lin S, Chen G, He L, Hu Z, Chan L, Trial J, Entman M, Wang Y
    J Am Soc Nephrol, 2013;24(10):1644-59.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  10. Vascular smooth muscle cell-derived adiponectin: a paracrine regulator of contractile phenotype.
    Authors: Ding M, Carrao A, Wagner R, Xie Y, Jin Y, Rzucidlo E, Yu J, Li W, Tellides G, Hwa J, Aprahamian T, Martin K
    J Mol Cell Cardiol, 2012;52(2):474-84.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  11. Molecular, biochemical and functional characterizations of C1q/TNF family members: adipose-tissue-selective expression patterns, regulation by PPAR-gamma agonist, cysteine-mediated oligomerizations, combinatorial associations and metabolic functions.
    Authors: Wong GW, Krawczyk SA, Kitidis-Mitrokostas C, Revett T, Gimeno R, Lodish HF
    Biochem. J., 2008;416(2):161-77.
    Species: Human
    Sample Type: Recombinant Protein
    Application: WB
  12. Adiponectin modulates inflammatory reactions via calreticulin receptor-dependent clearance of early apoptotic bodies.
    Authors: Takemura Y, Ouchi N, Shibata R, Aprahamian T, Kirber MT, Summer RS, Kihara S, Walsh K
    J. Clin. Invest., 2007;117(2):375-86.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
Expand to show all 12 Citations
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