Mouse Adiponectin/Acrp30 Antibody

Catalog # Availability Size / Price Qty
AF1119
AF1119-SP
Adiponectin/Acrp30 in ST-2 mouse bone marrow-derived stromal cell line differentiated to adipocytes.
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Product Details
Citations (16)
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Mouse Adiponectin/Acrp30 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Adiponectin/Acrp30 in direct ELISAs and Western blots. In direct ELISAs, greater than 50% cross-reactivity with recombinant rat Adiponectin is observed and less than 30% cross-reactivity with recombinant human Adiponectin is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Adiponectin/Acrp30 (R&D Systems, Catalog # 5095-AC)
Glu18-Asn247
Accession # Q60994
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Adiponectin/Acrp30 (Catalog # 5095-AC)
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunocytochemistry Adiponectin/Acrp30 in ST-2 mouse bone marrow-derived stromal cell line differentiated to adipocytes. View Larger

Adiponectin/Acrp30 in ST-2 mouse bone marrow-derived stromal cell line differentiated to adipocytes. Adiponectin/Acrp30 was detected in immersion fixed ST-2 mouse bone marrow-derived stromal cell line differentiated to adipocytes using Goat Anti-Mouse Adiponectin/Acrp30 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1119) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counter­stained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunocytochemistry Adiponectin/Acrp30 in human mesenchymal stem cells differentiated to adipocytes. View Larger

Adiponectin/Acrp30 in human mesenchymal stem cells differentiated to adipocytes. Adiponectin/Acrp30 was detected in immersion fixed human mesenchymal stem cells differentiated to adipocytes using Goat Anti-Mouse Adiponectin/Acrp30 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1119) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Adiponectin/Acrp30

Adiponectin, also known as Acrp30, is an adipocyte-derived protein with wide ranging paracrine and endocrine effects on metabolism and inflammation. It promotes adipocyte differentiation, fatty acid catabolism, and insulin sensitivity, and is negatively correlated with obesity, type 2 diabetes, and atherogenesis. In this context, adiponectin is an anti-inflammatory agent, but it exerts pro-inflammatory effects in nonmetabolic disorders such as rheumatoid arthritis and inflammatory bowel disease (1-3). Adiponectin interacts with the receptors AdipoR1 and AdipoR2, calreticulin, and Cadherin-13/T-Cadherin, as well as with several growth factors (4-7). Mature mouse adiponectin consists of a 66 amino acid (aa) N-terminal collagenous region and a 137 aa C-terminal C1q-like globular domain which can be cleaved by a leukocyte-derived elastase (8-10). Mature mouse adiponectin shares 83% and 91% amino acid (aa) sequence identity with human and rat adiponectin, respectively. Adiponectin associates into trimers that may assemble into medium molecular weight (MMW) hexamers and then into >300 kDa high molecular weight (HMW) oligomers (11-13). The glycosylation of four hydroxylated lysine residues in the collagenous domain is required for the intracellular formation of HMW complexes (14). The various multimeric forms of adiponectin exhibit distinct tissue specific and gender specific profiles and activities (13, 15).

References
  1. Lara-Castro, C. et al. (2007) Curr. Opin. Lipidol. 18:263.
  2. Tilg, H. and A.R. Moschen (2006) Nat. Rev. Immunol. 6:772.
  3. Fantuzzi, G. (2008) J. Allergy Clin. Immunol. 121:326.
  4. Yamauchi, T. et al. (2007) Nat. Med. 13:332.
  5. Takemura, Y. et al. (2007) J. Clin. Invest. 117:375.
  6. Hug, C. et al. (2004) Proc. Natl. Acad. Sci. USA 101:10308.
  7. Wang, Y. et al. (2005) J. Biol. Chem. 280:18341.
  8. Scherer, P.E. et al. (1995) J. Biol. Chem. 270:26746.
  9. Hu, E. et al. (1996) J. Biol. Chem. 271:10697.
  10. Waki, H. et al. (2005) Endocrinology 146:790.
  11. Waki, H. et al. (2003) J. Biol. Chem. 278:40352.
  12. Tsao, T.S. et al. (2003) J. Biol. Chem. 278:50810.
  13. Wang, Y. et al. (2008) Biochem. J. 409:623.
  14. Wang, H. et al. (2006) J. Biol. Chem. 281:16391.
  15. Pajvani, U.B. et al. (2003) J. Biol. Chem. 278:9073.
Entrez Gene IDs
9370 (Human); 11450 (Mouse); 246253 (Rat)
Alternate Names
ACDC; Acrp30; ACRP30ADPN; adipocyte, C1Q and collagen domain containing; Adiponectin; adiponectin, C1Q and collagen domain containing; AdipoQ; ADIPQTL1; ApM1; apM-1; APM1APM-1; C1q and collagen domain-containing protein; GBP28; GBP28apM1; Gelatin-binding protein

Product Datasheets

Citations for Mouse Adiponectin/Acrp30 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

16 Citations: Showing 1 - 10
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  1. Adiponectin modulates ventral tegmental area dopamine neuron activity and anxiety-related behavior through AdipoR1
    Authors: F Sun, Y Lei, J You, C Li, L Sun, J Garza, D Zhang, M Guo, PE Scherer, D Lodge, XY Lu
    Mol. Psychiatry, 2018;0(0):.
    Species: Mouse
    Sample Types: Plasma
    Applications: WB
  2. Adiponectin/T-cadherin system enhances exosome biogenesis and decreases cellular ceramides by exosomal release
    Authors: Y Obata, S Kita, Y Koyama, S Fukuda, H Takeda, M Takahashi, Y Fujishima, H Nagao, S Masuda, Y Tanaka, Y Nakamura, H Nishizawa, T Funahashi, B Ranscht, Y Izumi, T Bamba, E Fukusaki, R Hanayama, S Shimada, N Maeda, I Shimomura
    JCI Insight, 2018;3(8):.
    Species: Mouse
    Sample Types:
  3. Chronic administration of the angiotensin type 2 receptor agonist C21 improves insulin sensitivity in C57BL/6 mice
    Authors: DT Quiroga, MC Muñoz, C Gil, M Pffeifer, JE Toblli, UM Steckeling, JF Giani, FP Dominici
    Physiol Rep, 2018;6(16):e13824.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: WB
  4. The unique prodomain of T-cadherin plays a key role in adiponectin binding with the essential extracellular cadherin repeats 1 and 2
    Authors: S Fukuda, S Kita, Y Obata, Y Fujishima, H Nagao, S Masuda, Y Tanaka, H Nishizawa, T Funahashi, J Takagi, N Maeda, I Shimomura
    J. Biol. Chem, 2017;0(0):.
    Species: Mouse
    Sample Types: Serum
    Applications: WB
  5. Retinol-binding protein 7 is an endothelium-specific PPAR? cofactor mediating an antioxidant response through adiponectin
    Authors: C Hu, HL Keen, KT Lu, X Liu, J Wu, DR Davis, SC Ibeawuchi, S Vogel, FW Quelle, CD Sigmund
    JCI Insight, 2017;2(6):e91738.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. Chronic blockade of the AT2 receptor with PD123319 impairs insulin signaling in C57BL/6 mice
    Authors: M C Muñoz
    Peptides, 2016;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: WB
  7. Role of the adipose PPAR?-adiponectin axis in susceptibility to stress and depression/anxiety-related behaviors
    Mol. Psychiatry, 2016;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: WB
  8. Positive feedback regulation between adiponectin and T-cadherin impacts adiponectin levels in tissue and plasma of male mice.
    Authors: Matsuda, Keisuke, Fujishima, Yuya, Maeda, Norikazu, Mori, Takuya, Hirata, Ayumu, Sekimoto, Ryohei, Tsushima, Yu, Masuda, Shigeki, Yamaoka, Masaya, Inoue, Kana, Nishizawa, Hitoshi, Kita, Shunbun, Ranscht, Barbara, Funahashi, Tohru, Shimomura, Iichiro
    Endocrinology, 2015;156(3):934-46.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: WB
  9. Ultrastructural localization of adiponectin protein in vasculature of normal and atherosclerotic mice.
    Authors: Mori T, Koyama Y, Maeda N, Nakamura Y, Fujishima Y, Matsuda K, Funahashi T, Shimada S, Shimomura I
    Sci Rep, 2014;4(0):4895.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC OCT-embedded
  10. Adiponectin promotes monocyte-to-fibroblast transition in renal fibrosis.
    Authors: Yang J, Lin S, Chen G, He L, Hu Z, Chan L, Trial J, Entman M, Wang Y
    J Am Soc Nephrol, 2013;24(10):1644-59.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  11. Distinct fibroblast lineages determine dermal architecture in skin development and repair.
    Authors: Driskell R, Lichtenberger B, Hoste E, Kretzschmar K, Simons B, Charalambous M, Ferron S, Herault Y, Pavlovic G, Ferguson-Smith A, Watt F
    Nature, 2013;504(7479):277-81.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Frozen
  12. T-cadherin is essential for adiponectin-mediated revascularization.
    Authors: Parker-Duffen, Jennifer, Nakamura, Kazuto, Silver, Marcy, Kikuchi, Ryosuke, Tigges, Ulrich, Yoshida, Sumiko, Denzel, Martin S, Ranscht, Barbara, Walsh, Kenneth
    J Biol Chem, 2013;288(34):24886-97.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: WB
  13. Leucine-rich glioma inactivated 3 associates negatively with adiponectin.
    Authors: Kim H, Kwon N, Baek K, Kim D, Yun H
    Cytokine, 2013;62(2):206-9.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: WB
  14. Vascular smooth muscle cell-derived adiponectin: a paracrine regulator of contractile phenotype.
    Authors: Ding M, Carrao A, Wagner R, Xie Y, Jin Y, Rzucidlo E, Yu J, Li W, Tellides G, Hwa J, Aprahamian T, Martin K
    J Mol Cell Cardiol, 2012;52(2):474-84.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  15. Molecular, biochemical and functional characterizations of C1q/TNF family members: adipose-tissue-selective expression patterns, regulation by PPAR-gamma agonist, cysteine-mediated oligomerizations, combinatorial associations and metabolic functions.
    Authors: Wong GW, Krawczyk SA, Kitidis-Mitrokostas C, Revett T, Gimeno R, Lodish HF
    Biochem. J., 2008;416(2):161-77.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: WB
  16. Adiponectin modulates inflammatory reactions via calreticulin receptor-dependent clearance of early apoptotic bodies.
    Authors: Takemura Y, Ouchi N, Shibata R, Aprahamian T, Kirber MT, Summer RS, Kihara S, Walsh K
    J. Clin. Invest., 2007;117(2):375-86.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded

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Mouse Adiponectin/Acrp30 Antibody
By Anonymous on 04/26/2017