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Mouse Aminopeptidase N/CD13 Antibody

Catalog #: AF2335
28 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2335
AF2335-SP
Aminopeptidase N/CD13 in Mouse Splenocytes.
2 Images
Product Details
Citations (28)
FAQs
Supplemental Products
Reviews
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Aminopeptidase N/CD13 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Aminopeptidase N/CD13 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Aminopeptidase N/CD13
Lys69-Ser966
Accession # P97449
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Aminopeptidase N/CD13 (Catalog # 2335-ZN)
Flow Cytometry
2.5 µg/106 cells
bEnd.3 mouse endothelioma cell line
Immunohistochemistry
0.1-15 µg/mL
See below
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Mouse Aminopeptidase N/CD13 (Catalog # 2335-ZN), see our available Western blot detection antibodies
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry Aminopeptidase N/CD13 antibody in Mouse Splenocytes by Immunocytochemistry (ICC). View Larger

Aminopeptidase N/CD13 in Mouse Splenocytes. Aminopeptidase N/CD13 was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse Aminopeptidase N/CD13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2335) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunohistochemistry Aminopeptidase N/CD13 antibody in Mouse Kidney by Immunohistochemistry (IHC-P). View Larger

Aminopeptidase N/CD13 in Mouse Kidney. Aminopeptidase N/CD13 was detected in immersion fixed paraffin-embedded sections of mouse kidney using Goat Anti-Mouse Aminopeptidase N/CD13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2335) at 0.1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to apical membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Aminopeptidase N/CD13

The mouse Anpep gene encodes Aminopeptidase N (APN), which is also known as microsomal aminopeptiase, alanyl aminopeptidase, Aminopeptidase M, CD13, or membrane protein p161 (1‑3). The deduced amino acid sequence of mouse APN consists of a short cytoplasmic tail (residues 2 to 8), a transmembrane region (residue 9 to 32), a Ser/Thr rich region and a zinc metalloprotease domain (residues 69 to 966). Widely expressed in many cells, tissues and species, APN cleaves the N-terminal amino acids from bioactive peptides, leading to their inactivation or degradation. The roles of APN in many fields, such as neuroscience, hematopoeitic cells, immune system, angiogenesis, cancer and viral infection, have been reviewed (4).

References
  1. Chen, H. et al. (1996) J. Immunol. 157:2593.
  2. Larsen, S.L. et al. (1996) J. Exp. Med. 184:183.
  3. Hansen, A.S. et al. (1993) Eur. J. Immunol. 23:2358.
  4. Turner, A.J. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) p. 289 Academic Press, San Diego.
Entrez Gene IDs
290 (Human); 16790 (Mouse); 397520 (Porcine); 102115663 (Cynomolgus Monkey)
Alternate Names
alanyl (membrane) aminopeptidase; Alanyl aminopeptidase; Aminopeptidase M; Aminopeptidase N; ANPEP; AP-M; APN; AP-N; CD13 antigen; CD13; CD13APN; EC 3.4.11; EC 3.4.11.2; gp150; LAP1; Microsomal aminopeptidase; Myeloid plasma membrane glycoprotein CD13; p150; PEPN; PEPNhAPN

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Citations for Mouse Aminopeptidase N/CD13 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

28 Citations: Showing 1 - 10
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  1. T cell deficiency precipitates antibody evasion and emergence of neurovirulent polyomavirus
    Authors: MD Lauver, G Jin, KN Ayers, SN Carey, CS Specht, CS Abendroth, AE Lukacher
    Elife, 2022;11(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Divergent transcriptional regulation of astrocyte reactivity across disorders
    Authors: JE Burda, TM O'Shea, Y Ao, KB Suresh, S Wang, AM Bernstein, A Chandra, S Deverasett, R Kawaguchi, JH Kim, S McCallum, A Rogers, S Wahane, MV Sofroniew
    Nature, 2022;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Prenatal disruption of blood-brain barrier formation via cyclooxygenase activation leads to lifelong brain inflammation
    Authors: Q Zhao, W Dai, HY Chen, RE Jacobs, BV Zlokovic, BT Lund, A Montagne, A Bonnin
    Proceedings of the National Academy of Sciences of the United States of America, 2022;119(15):e2113310119.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-F
  4. Pericyte Loss Leads to Capillary Stalling Through Increased Leukocyte-Endothelial Cell Interaction in the Brain
    Authors: YG Choe, JH Yoon, J Joo, B Kim, SP Hong, GY Koh, DS Lee, WY Oh, Y Jeong
    Frontiers in Cellular Neuroscience, 2022;16(0):848764.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Prostacyclin Promotes Degenerative Pathology in a Model of Alzheimer's Disease
    Authors: TR Womack, CT Vollert, O Ohia-Nwoko, M Schmitt, S Montazari, TL Beckett, D Mayerich, MP Murphy, JL Eriksen
    Frontiers in Cellular Neuroscience, 2022;16(0):769347.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. Spatiotemporal reprogramming of differentiated cells underlies regeneration and neoplasia in the intestinal epithelium
    Authors: T Higa, Y Okita, A Matsumoto, S Nakayama, T Oka, O Sugahara, D Koga, S Takeishi, H Nakatsumi, N Hosen, S Robine, MM Taketo, T Sato, KI Nakayama
    Nature Communications, 2022;13(1):1500.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Adiponectin accumulation in the retinal vascular endothelium and its possible role in preventing early diabetic microvascular damage
    Authors: TA Sakaue, Y Fujishima, Y Fukushima, Y Tsugawa-Sh, S Fukuda, S Kita, H Nishizawa, B Ranscht, K Nishida, N Maeda, I Shimomura
    Scientific Reports, 2022;12(1):4159.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Mild traumatic brain injury induces microvascular injury and accelerates Alzheimer-like pathogenesis in mice
    Authors: Y Wu, H Wu, J Zeng, B Pluimer, S Dong, X Xie, X Guo, T Ge, X Liang, S Feng, Y Yan, JF Chen, N Sta Maria, Q Ma, F Gomez-Pini, Z Zhao
    Acta neuropathologica communications, 2021;9(1):74.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  9. Reactive astrocytes facilitate vascular repair and remodeling after stroke
    Authors: MR Williamson, CJA Fuertes, AK Dunn, MR Drew, TA Jones
    Cell Reports, 2021;35(4):109048.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  10. Capillary-associated microglia regulate vascular structure and function through PANX1-P2RY12 coupling in mice
    Authors: K Bisht, KA Okojie, K Sharma, DH Lentferink, YY Sun, HR Chen, JO Uweru, S Amancherla, Z Calcuttawa, AB Campos-Sal, B Corliss, L Jabbour, J Benderoth, B Friestad, WA Mills, BE Isakson, MÈ Tremblay, CY Kuan, UB Eyo
    Nature Communications, 2021;12(1):5289.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  11. Anatomical Organization of the Rat Subfornical Organ
    Authors: AI Hicks, S Kobrinsky, S Zhou, J Yang, M Prager-Kho
    Frontiers in Cellular Neuroscience, 2021;15(0):691711.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  12. Astrocyte-derived Wnt growth factors are required for endothelial blood-brain barrier maintenance
    Authors: S Guérit, E Fidan, J Macas, CJ Czupalla, R Figueiredo, A Vijikumar, BH Yalcin, S Thom, P Winter, H Gerhardt, K Devraj, S Liebner
    Progress in neurobiology, 2020;0(0):101937.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  13. Foreign body responses in mouse central nervous system mimic natural wound responses and alter biomaterial functions
    Authors: TM O?Shea, AL Wollenberg, JH Kim, Y Ao, TJ Deming, MV Sofroniew
    Nature Communications, 2020;11(1):6203.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  14. Fibrinogen induces neural stem cell differentiation into astrocytes in the subventricular zone via BMP signaling
    Authors: L Pous, SS Deshpande, S Nath, S Mezey, SC Malik, S Schildge, C Bohrer, K Topp, D Pfeifer, F Fernández-, S Doostkam, DK Galanakis, V Taylor, K Akassoglou, C Schachtrup
    Nat Commun, 2020;11(1):630.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  15. Precapillary sphincters maintain perfusion in the cerebral cortex
    Authors: S Grubb, C Cai, BO Hald, L Khennouf, RP Murmu, AGK Jensen, J Fordsmann, S Zambach, M Lauritzen
    Nat Commun, 2020;11(1):395.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC
  16. Blood brain barrier leakage is not a consistent feature of white matter lesions in CADASIL
    Authors: RM Rajani, J Ratelade, V Domenga-De, Y Hase, H Kalimo, RN Kalaria, A Joutel
    Acta Neuropathol Commun, 2019;7(1):187.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  17. GPR124 facilitates pericyte polarization and migration by regulating the formation of filopodia during ischemic injury
    Authors: DY Chen, NH Sun, YP Lu, LJ Hong, TT Cui, CK Wang, XH Chen, SS Wang, LL Feng, WX Shi, K Fukunaga, Z Chen, YM Lu, F Han
    Theranostics, 2019;9(20):5937-5955.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  18. Single-cell transcriptomes of the regenerating intestine reveal a revival stem cell
    Authors: A Ayyaz, S Kumar, B Sangiorgi, B Ghoshal, J Gosio, S Ouladan, M Fink, S Barutcu, D Trcka, J Shen, K Chan, JL Wrana, A Gregorieff
    Nature, 2019;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  19. Microglia are an essential component of the neuroprotective scar that forms after spinal cord injury
    Authors: V Bellver-La, F Bretheau, B Mailhot, N Vallières, M Lessard, ME Janelle, N Vernoux, MÈ Tremblay, T Fuehrmann, MS Shoichet, S Lacroix
    Nat Commun, 2019;10(1):518.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  20. Endothelial Calcineurin Signaling Restrains Metastatic Outgrowth by Regulating Bmp2
    Authors: S Hendrikx, S Coso, B Prat-Luri, L Wetterwald, A Sabine, CA Franco, S Nassiri, N Zangger, H Gerhardt, M Delorenzi, TV Petrova
    Cell Rep, 2019;26(5):1227-1241.e6.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  21. Thalidomide Reduces Hemorrhage of Brain Arteriovenous Malformations in a Mouse Model
    Authors: W Zhu, W Chen, D Zou, L Wang, C Bao, L Zhan, D Saw, S Wang, E Winkler, Z Li, M Zhang, F Shen, S Shaligram, M Lawton, H Su
    Stroke, 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  22. Blood-brain barrier-associated pericytes internalize and clear aggregated amyloid-?42 by LRP1-dependent apolipoprotein E isoform-specific mechanism
    Authors: Q Ma, Z Zhao, AP Sagare, Y Wu, M Wang, NC Owens, PB Verghese, J Herz, DM Holtzman, BV Zlokovic
    Mol Neurodegener, 2018;13(1):57.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  23. Apo?4 disrupts neurovascular regulation and undermines white matter integrity and cognitive function
    Authors: K Koizumi, Y Hattori, SJ Ahn, I Buendia, A Ciacciarel, K Uekawa, G Wang, A Hiller, L Zhao, HU Voss, SM Paul, C Schaffer, L Park, C Iadecola
    Nat Commun, 2018;9(1):3816.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: ICC
  24. Required growth facilitators propel axon regeneration across complete spinal cord injury
    Authors: MA Anderson, TM O'Shea, JE Burda, Y Ao, SL Barlatey, AM Bernstein, JH Kim, ND James, A Rogers, B Kato, AL Wollenberg, R Kawaguchi, G Coppola, C Wang, TJ Deming, Z He, G Courtine, MV Sofroniew
    Nature, 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  25. Regional early and progressive loss of brain pericytes but not vascular smooth muscle cells in adult mice with disrupted platelet-derived growth factor receptor-? signaling
    Authors: AM Nikolakopo, Z Zhao, A Montagne, BV Zlokovic
    PLoS ONE, 2017;12(4):e0176225.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  26. Prostacyclin prevents pericyte loss and demyelination induced by lysophosphatidylcholine in the central nervous system.
    Authors: Muramatsu R, Kuroda M, Matoba K, Lin H, Takahashi C, Koyama Y, Yamashita T
    J Biol Chem, 2015;290(18):11515-25.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  27. Adiponectin expression protects against angiotensin II-mediated inflammation and accelerated atherosclerosis.
    Authors: van Stijn, Caroline, Kim, Jason, Barish, Grant D, Tietge, Uwe J F, Tangirala, Rajendra
    PLoS ONE, 2014;9(1):e86404.
    Species: Mouse
    Sample Types: Plasma
    Applications: Western Blot
  28. Pericyte loss influences Alzheimer-like neurodegeneration in mice.
    Authors: Sagare A, Bell R, Zhao Z, Ma Q, Winkler E, Ramanathan A, Zlokovic B
    Nat Commun, 2013;4(0):2932.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr

FAQs

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