Mouse CCL2/JE/MCP-1 Antibody

Catalog # Availability Size / Price Qty
AF-479-NA
AF-479-SP
Detection  of Recombinant Mouse CCL2/JE/MCP‑1 by Western Blot.
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Product Details
Citations (37)
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Mouse CCL2/JE/MCP-1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CCL2/JE/MCP-1 in ELISAs and Western blots. In sandwich immunoassays, less than 0.01% cross-reactivity with recombinant human (rh) MCP‑1, rhMCP‑2, rhMCP-3, recombinant mouse (rm) MARC, rhMCP-4, rmMCP-5, rmMDC, rmMIP-1 alpha, rmMIP-1 beta, rmMIP-1 gamma,
rmMIP‑2, rmMIP‑3 beta, rmRANTES, rmTARC, and rmTeck is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse CCL2/JE/MCP-1
Gln24-Arg96
Accession # Q5SVU3
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below

Mouse CCL2/JE/MCP-1 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Mouse CCL2/JE/MCP‑1 Biotinylated Antibody (Catalog # BAF479)

Standard: Recombinant Mouse CCL2/JE/MCP-1 Protein (Catalog # 479-JE)

Neutralization
Measured by its ability to neutralize CCL2/JE/MCP‑1-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR2A. The Neutralization Dose (ND50) is typically 0.5-2.5 µg/mL in the presence of 0.04 µg/mL Recombinant Mouse CCL2/JE/MCP‑1.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection  of Recombinant Mouse CCL2/JE/MCP‑1 by Western Blot. View Larger

Detection of Recombinant Mouse CCL2/JE/MCP‑1 by Western Blot. Western blot shows 25 ng of Recombinant Mouse CCL2/JE/MCP‑1 (Catalog # 479-JE), Recombinant Human CCL2/JE/MCP‑1 (Catalog # 279-MC), Recombinant Rat CCL2/JE/MCP‑1 (Catalog # 3144-JE), Recombinant Mouse CCL11/Eotaxin (Catalog # 420-ME), Recombinant Mouse CCL12/MCP‑5 (Catalog # 428-P5), and Recombinant Mouse CCL7/MCP‑3/MARC (Catalog # 456-MC). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Mouse CCL2/JE/MCP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-479-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for CCL2/JE/
MCP‑1 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Neutralization Chemotaxis Induced by CCL2/JE and Neutralization by Mouse CCL2/JE Antibody. View Larger

Chemotaxis Induced by CCL2/JE and Neutralization by Mouse CCL2/JE Antibody. Recombinant Mouse CCL2/JE (Catalog # 479-JE) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL2/JE (0.04 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL2/JE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-479-NA). The ND50 is typically 0.5-2.5 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL2/JE/MCP-1

Mouse CCL2 is a member of the beta (C-C) subfamily of chemokines. The murine CCL2 gene was initially identified as a platelet-derived growth factor-inducible gene in murine fibroblasts. Mouse CCL2 cDNA encodes a 148 amino acid (aa) residue with a putative 23 aa signal peptide that is cleaved to generate the mature protein. Mouse CCL2 shares 82% amino acid sequence identity with rat CCL2. Mouse CCL2 also shares 55% amino acid sequence identity with human MCP-1. Compared to human MCP-1, mouse CCL2 has a 49 aa residue extension at the carboxy-terminus. Mouse CCL2 has full activity on human cells while human MCP-1 has limited activity on mouse cells.

Entrez Gene IDs
6347 (Human); 20296 (Mouse); 24770 (Rat); 403981 (Canine)
Alternate Names
C-C motif chemokine ligand 2; CCL2; GDCF-2; HC11; HSMCR30; MCAF; Mcp1; MCP-1; SCYA2; SMC-CF

Product Datasheets

Citations for Mouse CCL2/JE/MCP-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

37 Citations: Showing 1 - 10
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  1. Hepatocyte nuclear receptor SHP suppresses inflammation and fibrosis in a mouse model of nonalcoholic steatohepatitis
    Authors: A Zou, N Magee, F Deng, S Lehn, C Zhong, Y Zhang
    J. Biol. Chem., 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neut
  2. The Chemokine CCL2 Mediates the Seizure-enhancing Effects of Systemic Inflammation
    Authors: C Cerri, S Genovesi, M Allegra, F Pistillo, U Püntener, A Guglielmot, VH Perry, Y Bozzi, M Caleo
    J Neurosci, 2016;36(13):3777-88.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In vivo
  3. Vitamin D limits chemokine expression in adipocytes and macrophage migration in vitro and in male mice.
    Authors: Karkeni E, Marcotorchino J, Tourniaire F, Astier J, Peiretti F, Darmon P, Landrier J
    Endocrinology, 2015;156(5):1782-93.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neut
  4. P2RX7 sensitizes Mac-1/ICAM-1-dependent leukocyte-endothelial adhesion and promotes neurovascular injury during septic encephalopathy.
    Authors: Wang H, Hong L, Huang J, Jiang Q, Tao R, Tan C, Lu N, Wang C, Ahmed M, Lu Y, Liu Z, Shi W, Lai E, Wilcox C, Han F
    Cell Res, 2015;25(6):674-90.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In vivo
  5. IL-1beta mediates MCP-1 induction by Wnt5a in gastric cancer cells.
    Authors: Li S, Wang W, Zhang N, Ma T, Zhao C
    BMC Cancer, 2014;14(0):480.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  6. GEC-derived SFRP5 inhibits Wnt5a-induced macrophage chemotaxis and activation.
    Authors: Zhao C, Bu X, Wang W, Ma T, Ma H
    PLoS ONE, 2014;9(1):e85058.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  7. Persistent inflammation subverts thrombospondin-1-induced regulation of retinal angiogenesis and is driven by CCR2 ligation.
    Authors: Chen M, Copland DA, Zhao J, Liu J, Forrester JV, Dick AD, Xu H
    Am. J. Pathol., 2012;180(1):235-45.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Frozen
  8. Response patterns of cytokines/chemokines in two murine strains after irradiation.
    Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
    Cytokine, 2012;58(2):169-77.
    Species: Mouse
    Sample Types: Plasma
    Applications: Luminex Assay Development
  9. Toll-like receptor 2 contributes to chemokine gene expression and macrophage infiltration in the dorsal root ganglia after peripheral nerve injury.
    Authors: Kim D, You B, Lim H, Lee SJ
    Mol Pain, 2011;7(0):74.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  10. Bovine lactoferrin counteracts Toll-like receptor mediated activation signals in antigen presenting cells.
    Authors: Puddu P, Latorre D, Carollo M, Catizone A, Ricci G, Valenti P, Gessani S
    PLoS ONE, 2011;6(7):e22504.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  11. Comprehensive assessment of chemokine expression profiles by flow cytometry.
    Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
    J. Clin. Invest., 2010;120(3):907-23.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow
  12. Stromal MCP-1 in mammary tumors induces tumor-associated macrophage infiltration and contributes to tumor progression.
    Authors: Fujimoto H, Sangai T, Ishii G, Ikehara A, Nagashima T, Miyazaki M, Ochiai A
    Int. J. Cancer, 2009;125(6):1276-84.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In vivo
  13. Resistance of human alveolar macrophages to Bacillus anthracis lethal toxin.
    Authors: Wu W, Mehta H, Chakrabarty K, Booth JL, Duggan ES, Patel KB, Ballard JD, Coggeshall KM, Metcalf JP
    J. Immunol., 2009;183(9):5799-806.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  14. Role of the chemokine decoy receptor D6 in balancing inflammation, immune activation, and antimicrobial resistance in Mycobacterium tuberculosis infection.
    Authors: Di Liberto D, Locati M, Caccamo N, Vecchi A, Meraviglia S, Salerno A, Sireci G, Nebuloni M, Caceres N, Cardona PJ, Dieli F, Mantovani A
    J. Exp. Med., 2008;205(9):2075-84.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In vivo
  15. Expanded-polyglutamine huntingtin protein suppresses the secretion and production of a chemokine (CCL5/RANTES) by astrocytes.
    Authors: Chou SY, Weng JY, Lai HL, Liao F, Sun SH, Tu PH, Dickson DW, Chern Y
    J. Neurosci., 2008;28(13):3277-90.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  16. Megalin contributes to the early injury of proximal tubule cells during nonselective proteinuria.
    Authors: Motoyoshi Y, Matsusaka T, Saito A, Pastan I, Willnow TE, Mizutani S, Ichikawa I
    Kidney Int., 2008;74(10):1262-9.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded
  17. Vascular and inflammatory stresses mediate atherosclerosis via RAGE and its ligands in apoE-/- mice.
    Authors: Harja E, Bu DX, Hudson BI, Chang JS, Shen X, Hallam K, Kalea AZ, Lu Y, Rosario RH, Oruganti S, Nikolla Z, Belov D, Lalla E, Ramasamy R, Yan SF, Schmidt AM
    J. Clin. Invest., 2008;118(1):183-94.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: WB
  18. Reversal of axonal loss and disability in a mouse model of progressive multiple sclerosis.
    Authors: Basso AS, Frenkel D, Quintana FJ, Costa-Pinto FA, Petrovic-Stojkovic S, Puckett L, Monsonego A, Bar-Shir A, Engel Y, Gozin M, Weiner HL
    J. Clin. Invest., 2008;118(4):1532-43.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC Frozen
  19. CD14 contributes to pulmonary inflammation and mortality during murine tuberculosis.
    Authors: Wieland CW, van der Windt GJ, Wiersinga WJ, Florquin S, van der Poll T
    Immunology, 2008;125(2):272-9.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA Development
  20. Thrombin induces fibroblast CCL2/JE production and release via coupling of PAR1 to Galphaq and cooperation between ERK1/2 and Rho kinase signaling pathways.
    Authors: Deng X, Mercer PF, Scotton CJ, Gilchrist A, Chambers RC
    Mol. Biol. Cell, 2008;19(6):2520-33.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  21. Chemical interactions between fibrosarcoma cancer cells and sensory neurons contribute to cancer pain.
    Authors: Khasabova IA, Stucky CL, Harding-Rose C, Eikmeier L, Beitz AJ, Coicou LG, Hanson AE, Simone DA, Seybold VS
    J. Neurosci., 2007;27(38):10289-98.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Frozen
  22. TWEAK/Fn14 interactions are instrumental in the pathogenesis of nephritis in the chronic graft-versus-host model of systemic lupus erythematosus.
    Authors: Zhao Z, Burkly LC, Campbell S, Schwartz N, Molano A, Choudhury A, Eisenberg RA, Michaelson JS, Putterman C
    J. Immunol., 2007;179(11):7949-58.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded
  23. An inflammation-induced mechanism for leukocyte transmigration across lymphatic vessel endothelium.
    Authors: Johnson LA, Clasper S, Holt AP, Lalor PF, Baban D, Jackson DG
    J. Exp. Med., 2006;203(12):2763-77.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Frozen
  24. Adenoviral-mediated overexpression of SOCS3 enhances IgG immune complex-induced acute lung injury.
    Authors: Gao H, Hoesel LM, Guo RF, Rancilio NJ, Sarma JV, Ward PA
    J. Immunol., 2006;177(1):612-20.
    Species: Human
    Sample Types: BALF
    Applications: ELISA Development
  25. CCL2 regulates angiogenesis via activation of Ets-1 transcription factor.
    Authors: Stamatovic SM, Keep RF, Mostarica-Stojkovic M, Andjelkovic AV
    J. Immunol., 2006;177(4):2651-61.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neut
  26. Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality.
    Authors: Osuchowski MF, Welch K, Siddiqui J, Remick DG
    J. Immunol., 2006;177(3):1967-74.
    Species: Mouse
    Sample Types: Plasma
    Applications: ELISA Development
  27. The chemokine decoy receptor M3 blocks CC chemokine ligand 2 and CXC chemokine ligand 13 function in vivo.
    Authors: Martin AP, Canasto-Chibuque C, Shang L, Rollins BJ, Lira SA
    J. Immunol., 2006;177(10):7296-302.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded
  28. Sequential ELISA to profile multiple cytokines from small volumes.
    Authors: Osuchowski MF, Siddiqui J, Copeland S, Remick DG
    J. Immunol. Methods, 2005;302(1):172-81.
    Species: Mouse
    Sample Types: Plasma
    Applications: ELISA Development
  29. Involvement of monocyte chemoattractant protein-1, macrophage inflammatory protein-1alpha and interleukin-1beta in Wallerian degeneration.
    Authors: Perrin FE, Lacroix S, Aviles-Trigueros M, David S
    Brain, 2005;128(0):854-66.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In vivo
  30. Antibody-bound beta-amyloid precursor protein stimulates the production of tumor necrosis factor-alpha and monocyte chemoattractant protein-1 by cortical neurons.
    Authors: Gonzalez de Aguilar JL, Dupuis L, Larmet Y
    Neurobiol. Dis., 2005;19(1):129-41.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: WB
  31. Selective macrophage suppression during sepsis.
    Authors: Ellaban E, Bolgos G, Remick D
    Cell. Immunol., 2004;231(1):103-11.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  32. A critical role for CCR2/MCP-1 interactions in the development of idiopathic pneumonia syndrome after allogeneic bone marrow transplantation.
    Authors: Hildebrandt GC, Duffner UA, Olkiewicz KM, Corrion LA, Willmarth NE, Williams DL, Clouthier SG, Hogaboam CM, Reddy PR, Moore BB, Kuziel WA, Liu C, Yanik G, Cooke KR
    Blood, 2004;103(6):2417-26.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  33. 17beta-estradiol (E2) modulates cytokine and chemokine expression in human monocyte-derived dendritic cells.
    Authors: Bengtsson AK, Ryan EJ, Giordano D, Magaletti DM, Clark EA
    Blood, 2004;104(5):1404-10.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  34. Possible co-regulation of genes associated with enhanced progression of mammary adenocarcinomas.
    Authors: Neumark E, Cohn MA, Lukanidin E, Witz IP, Ben-Baruch A
    Immunol. Lett., 2002;82(1):111-21.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: DB
  35. The chemokine KC, but not monocyte chemoattractant protein-1, triggers monocyte arrest on early atherosclerotic endothelium.
    Authors: Huo Y, Weber C, Forlow SB, Sperandio M, Thatte J, Mack M, Jung S, Littman DR, Ley K
    J. Clin. Invest., 2001;108(9):1307-14.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neut
  36. Role of CC chemokines (macrophage inflammatory protein-1 beta, monocyte chemoattractant protein-1, RANTES) in acute lung injury in rats.
    Authors: Bless NM, Huber-Lang M, Guo RF, Warner RL, Schmal H, Czermak BJ, Shanley TP, Crouch LD, Lentsch AB, Sarma V, Mulligan MS, Friedl HP, Ward PA
    J. Immunol., 2000;164(5):2650-9.
    Species: Rat
    Sample Types: BALF
    Applications: ELISA Development
  37. Pulmonary Epithelial Cell-Derived Cytokine TGF-beta1 Is a Critical Cofactor for Enhanced Innate Lymphoid Cell Function.
    Authors: Denney L, Byrne A, Shea T, Buckley J, Pease J, Herledan G, Walker S, Gregory L, Lloyd C
    Immunity, 0;43(5):945-58.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA Capture

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