Mouse IL-4 Antibody

(3 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse IL-4 in direct ELISAs and Western blots. In Western blots, approximately 10% cross-reactivity with recombinant rat IL‑4 and less than 1% cross-reactivity with recombinant human IL‑4, recombinant porcine IL‑4, and recombinant cotton rat IL‑4 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant mouse IL-4
    His23-Ser140
    Accession # P07750
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse IL‑4 (Catalog # 404-ML)
  • Neutralization
    Measured by its ability to neutralize IL‑4-induced proliferation in the HT‑2 mouse T cell line. The Neutralization Dose (ND50) is typically 0.1-0.3 µg/mL in the presence of 7.5 ng/mL Recombinant Mouse IL‑4.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Cell Proliferation Induced by IL‑4 and Neutralization by Mouse IL‑4 Antibody. Recombinant Mouse IL‑4 (Catalog # 404-ML) stimulates proliferation in the HT‑2 mouse T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑4 (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-404-NA). The ND50 is typically 0.1-0.3 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-4

Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13-18 kDa Th2 cytokine that shows pleiotropic effects during immune responses (1‑4). It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha -helix structure (5). Mouse IL-4 is synthesized with a 24 amino acid (aa) signal sequence. Mature mouse IL-4 shares 39%, 39%, and 59% aa sequence identity with bovine, human, and rat IL-4, respectively. Human, mouse, and rat IL-4 are species-specific in their activities (6-8). IL-4 exerts its effects through two receptor complexes (9, 10). The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and -21). The type II receptor on nonhematopoietic cells consists of IL-4 R alpha and IL-13 R alpha 1. The type II receptor also transduces IL-13 mediated signals. IL-4 is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils (1, 2). It promotes cell proliferation, survival, and immunoglobulin class switch to IgG1 and IgE in mouse B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells (11-14). IL-4 plays a dominant role in the development of allergic inflammation and asthma (13, 15).

  • References:
    1. Benczik, M. and S.L. Gaffen (2004) Immunol. Invest. 33:109.
    2. Chomarat, P. and J. Banchereau (1998) Int. Rev. Immunol. 17:1.
    3. Lee, F. et al. (1986) Proc. Natl. Acad. Sci. USA 83:2061.
    4. Noma, Y. et al. (1986) Nature 319:640.
    5. Redfield, C. et al. (1991) Biochemistry 30:11029.
    6. Ramirez, F. et al. (1988) J. Immunol. Meth. 221:141.
    7. Leitenberg, D. and T.L. Feldbush (1988) Cell. Immunol. 111:451.
    8. Mosman, T.R. et al. (1987) J. Immunol. 138:1813.
    9. Mueller, T.D. et al. (2002) Biochim. Biophys. Acta 1592:237.
    10. Nelms, K. et al. (1999) Annu. Rev. Immunol. 17:701.
    11. Paludan, S.R. (1998) Scand. J. Immunol. 48:459.
    12. Corthay, A. (2006) Scand. J. Immunol. 64:93.
    13. Ryan, J.J. et al. (2007) Crit. Rev. Immunol. 27:15.
    14. Grone, A. (2002) Vet. Immunol. Immunopathol. 88:1.
    15. Rosenberg, H.F. et al. (2007) J. Allergy Clin. Immunol. 119:1303.
  • Long Name:
    Interleukin 4
  • Entrez Gene IDs:
    3565 (Human); 16189 (Mouse); 287287 (Rat); 397225 (Porcine); 280824 (Bovine); 403785 (Canine); 574281 (Primate); 100302454 (Rabbit)
  • Alternate Names:
    B cell growth factor 1; BCDF; B-cell stimulatory factor 1; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; IL4; IL-4; IL-4B_cell stimulatory factor 1; interleukin 4; interleukin-4; Lymphocyte stimulatory factor 1; MGC79402; pitrakinra
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Applications
Sample Type
  1. The IL-4/IL-13/Stat6 signalling pathway promotes luminal mammary epithelial cell development.
    Authors: Khaled WT, Read EK, Nicholson SE, Baxter FO, Brennan AJ, Came PJ, Sprigg N, McKenzie AN, Watson CJ
    Development, 2007;134(15):2739-50.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  2. Optimizing plasmid-based gene transfer for investigating skeletal muscle structure and function.
    Authors: Schertzer JD, Plant DR, Lynch GS
    Mol. Ther., 2006;13(4):795-803.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  3. The IL-6 family cytokines, interleukin-6, interleukin-11, oncostatin M, and leukemia inhibitory factor, enhance mast cell growth through fibroblast-dependent pathway in mice.
    Authors: Gyotoku E, Morita E, Kameyoshi Y, Hiragun T, Yamamoto S, Hide M
    Arch. Dermatol. Res., 2001;293(10):508-14.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
Isotype Controls
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Goat IgG (H+L) APC-conjugated Antibody

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Donkey Anti-Goat IgG Antibody

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Goat IgG (H+L) Fluorescein-conjugated Antibody

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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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