Mouse IL-6R alpha Antibody
Mouse IL-6R alpha Antibody Summary
Leu20-Glu357
Accession # P22272
Applications
Mouse IL-6R alpha Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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IL-6R alpha in Mouse Thymus. IL-6R alpha was detected in perfusion fixed frozen sections of mouse thymus using Mouse IL-6R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1830) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
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IL-6R alpha in Mouse Thymus. IL-6R alpha was detected in perfusion fixed frozen sections of mouse thymus using Mouse IL-6R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1830) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
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Cell Proliferation Induced by IL‑6 and Neutralization by Mouse IL-6R alpha Antibody. Recombinant Mouse IL-6 (Catalog # 406-ML) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-6 (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL-6R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1830). The ND50 is typically 0.2-1 µg/mL.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-6R alpha
Interleukin 6 (IL-6) is a multifunctional cytokine that exerts its activities by binding to a high-affinity receptor complex consisting of two membrane glycoproteins: an 80 kDa ligand binding subunit (IL-6 R alpha /CD126) and a 130 kDa nonligand-binding signal-transducing subunit (gp130/CD130) (1‑4). The mouse IL-6 R alpha cDNA encodes a precursor type I transmembrane protein of 460 amino acids (aa) that contains a 19 aa signal sequence, a 345 aa extracellular ligand binding domain, a 21 aa transmembrane region, and a 75 aa cytoplasmic segment (2). The extracellular segment contains an Ig-like and a fibronectin-type III domain, plus a membrane proximal WSXWS motif. In their extracellular regions, mouse IL-6 R alpha shares 89%, 51% and 50% aa identity with rat, human and porcine IL-6 R alpha, respectively. Unlike gp130 that is expressed ubiquitously, the cellular distribution of IL-6 R alpha is predominantly limited to hepatocytes and leukocyte subpopulations such as monocytes, neutrophils, T and B cells. Soluble IL-6 R alpha has been found in various body fluids (5). Two soluble receptor isoforms that arise either from proteolytic cleavage of the membrane-bound IL-6 R alpha, or by alternative mRNA splicing (reported only in human) have been described (6, 7). Soluble IL-6 R alpha binds IL-6 with an affinity similar to that of the membrane-bound IL-6 R alpha. More importantly, the soluble IL-6 R alpha /IL-6 complex is capable of interacting with the membrane-bound gp130 to activate cells that lack an integral membrane IL-6 R alpha. It has been documented that elevated soluble IL-6 R is associated with numerous diseases including arthritic lesions, multiple myeloma and Crohn’s disease (6, 7).
- Yamasaki, K. et al. (1988) Science 241:825.
- Sugita, T. et al. (1990) J. Exp. Med. 171:2001.
- Hibi, M. et al. (1990) Cell 63:1149.
- Saito, M. et al. (1992) J. Immunol. 148:4066.
- Novick, D. et al. (1989) J. Exp. Med. 170:1409.
- Jones, S.A. et al. (2001) FASEB J. 15:43.
- Jones, S.A. and S. Rose-John (2002) Biochim. Biophys. Acta 1592:251.
Product Datasheets
Citations for Mouse IL-6R alpha Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
15
Citations: Showing 1 - 10
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Increased cytokine expression in the choroid plexus stroma and epithelium in response to endotoxin-induced systemic inflammation in mice
Authors: A Shimada, S Hasegawa-I
Toxicology reports, 2021-03-10;8(0):520-528.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Chromatin Regulator, CHD1, Remodels the Immunosuppressive Tumor Microenvironment in PTEN-Deficient Prostate Cancer
Authors: D Zhao, L Cai, X Lu, X Liang, J Li, P Chen, M Ittmann, X Shang, S Jiang, H Li, C Meng, I Flores, JH Song, JW Horner, Z Lan, CJ Wu, J Li, Q Chang, KC Chen, G Wang, P Deng, DJ Spring, YA Wang, RA DePinho
Cancer Discov, 2020-05-08;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Extracellular CIRP induces macrophage endotoxin tolerance through IL-6R-mediated STAT3 activation
Authors: M Zhou, M Aziz, NL Denning, HT Yen, G Ma, P Wang
JCI Insight, 2020-03-12;0(0):.
Species: Mouse
Sample Types: In Vivo, Whole Cells
Applications: Flow Cytometry, ICC, Neutralization -
Targeting TGFbetaR2-mutant tumors exposes vulnerabilities to stromal TGFbeta blockade in pancreatic cancer
Authors: H Huang, Y Zhang, V Gallegos, N Sorrelle, MM Zaid, J Toombs, W Du, S Wright, M Hagopian, Z Wang, AN Hosein, AA Sathe, C Xing, EJ Koay, KE Driscoll, RA Brekken
EMBO Mol Med, 2019-10-14;0(0):e10515.
Species: Human, Mouse
Sample Types: Protein Lysates, Whole Tissue
Applications: IHC-P, Western Blot -
Role of Proinflammatory Cytokines in Feedback Modulation of Circadian Clock Gene Rhythms by Saturated Fatty Acids
Authors: SM Kim, N Neuendorff, DJ Earnest
Sci Rep, 2019-06-20;9(1):8909.
Species: Mouse
Sample Types: Transduced Whole Cells
Applications: Neutralization -
STAT-3-independent production of IL-17 by mouse innate-like ?? T cells controls ocular infection
Authors: AJ St Leger, AM Hansen, H Karauzum, R Horai, CR Yu, A Laurence, KD Mayer-Barb, P Silver, R Villasmil, C Egwuagu, SK Datta, RR Caspi
J. Exp. Med., 2018-02-28;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization -
Rapid monocyte infiltration following retinal detachment is dependent on non-canonical IL6 signaling through gp130
Authors: X Wang, EB Miller, M Goswami, P Zhang, KE Ronning, SJ Karlen, RJ Zawadzki, EN Pugh, ME Burns
J Neuroinflammation, 2017-06-23;14(1):121.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
Meprin Metalloproteases Generate Biologically Active Soluble Interleukin-6 Receptor to Induce Trans-Signaling
Authors: P Arnold, I Boll, M Rothaug, N Schumacher, F Schmidt, R Wichert, J Schneppenh, J Lokau, U Pickhinke, T Koudelka, A Tholey, B Rabe, J Scheller, R Lucius, C Garbers, S Rose-John, C Becker-Pau
Sci Rep, 2017-03-09;7(0):44053.
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Macrophagic control of the response to uropathogenic E. coli infection by regulation of iron retention in an IL-6-dependent manner
Authors: Nana Owusu-Boai
Immun Inflamm Dis, 2016-08-28;4(4):413-426.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Species Specificity of ADAM10 and ADAM17 Proteins in Interleukin-6 (IL-6) Trans-signaling and Novel Role of ADAM10 in Inducible IL-6 Receptor Shedding.
Authors: Garbers C, Janner N, Chalaris A, Moss ML, Floss DM, Meyer D, Koch-Nolte F, Rose-John S, Scheller J
J. Biol. Chem., 2011-03-15;286(17):14804-11.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: Western Blot -
IL6/sIL6R complex contributes to emergency granulopoietic responses in G-CSF- and GM-CSF-deficient mice.
Authors: Walker F, Zhang HH, Matthews V, Weinstock J, Nice EC, Ernst M, Rose-John S, Burgess AW
Blood, 2007-12-21;111(8):3978-85.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: Western Blot -
Transgenic blockade of interleukin 6 transsignaling abrogates inflammation.
Authors: Rabe B, Chalaris A, May U, Waetzig GH, Seegert D, Williams AS, Jones SA, Rose-John S, Scheller J
Blood, 2007-11-07;111(3):1021-8.
Species: Mouse
Sample Types: Air Pouch Exudate
Applications: ELISA Development -
Apoptosis is a natural stimulus of IL6R shedding and contributes to the proinflammatory trans-signaling function of neutrophils.
Authors: Chalaris A, Rabe B, Paliga K, Lange H, Laskay T, Fielding CA, Jones SA, Rose-John S, Scheller J
Blood, 2007-06-13;110(6):1748-55.
Species: Mouse
Sample Types: Lavage Fluid
Applications: ELISA Development -
Inactivated Sendai virus particles eradicate tumors by inducing immune responses through blocking regulatory T cells.
Authors: Kurooka M, Kaneda Y
Cancer Res., 2007-01-01;67(1):227-36.
Species: Mouse
Sample Types:
Applications: Neutralization -
Sequential ELISA to profile multiple cytokines from small volumes.
Authors: Osuchowski MF, Siddiqui J, Copeland S, Remick DG
J. Immunol. Methods, 2005-07-01;302(1):172-81.
Species: Mouse
Sample Types: Plasma
Applications: ELISA Development
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