Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Porcine, Equine

Cited:

Equine

Applications

Validated:

Western Blot, Flow Cytometry, CyTOF-ready

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 433423
View all CD14 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant porcine CD14

Specificity

Detects porcine CD14 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human CD14 and recombinant mouse CD14 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Porcine/Equine CD14 Antibody

Detection of CD14 antibody in Porcine PBMCs antibody by Flow Cytometry.

Detection of CD14 in Porcine PBMCs by Flow Cytometry.

Porcine peripheral blood mononuclear cells were stained with Mouse Anti-Porcine/Equine CD14 Monoclonal Antibody (Catalog # MAB4597, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B).
Detection of CD14 antibody in Equine PBMCs antibody by Flow Cytometry.

Detection of CD14 in Equine PBMCs by Flow Cytometry.

Equine peripheral blood mononuclear cells (PBMCs) treated with PMA and Calcium Ionomycin for 24 hours were stained with Mouse Anti-Porcine/Equine CD14 Monoclonal Antibody (Catalog # MAB4597, filled histogram) or isotype control antibody (Catalog # MAB004, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Applications for Porcine/Equine CD14 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: Porcine peripheral blood mononuclear cells (untreated) and equine peripheral blood mononuclear cells (PBMCs) treated with PMA and Calcium Ionomycin

Western Blot

1 µg/mL
Sample: Recombinant Porcine CD14

Reviewed Applications

Read 1 review rated 4 using MAB4597 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD14

CD14 is a 55 kDa GPI-linked cell surface glycoprotein that is preferentially expressed on monocytes/macrophages. It is a pattern recognition protein that acts as a receptor for lipopolysaccharide (LPS) and other microbial cell wall components. CD14 also occurs as a secreted protein. Mature porcine CD14 shares 70‑75% aa identity with human, cow, sheep, water buffalo and horse CD14 and 61‑62% aa identity with mouse and rat CD14.

Alternate Names

CD14

Entrez Gene IDs

929 (Human); 12475 (Mouse); 60350 (Rat); 100037938 (Porcine)

Gene Symbol

CD14

Additional CD14 Products

Product Documents for Porcine/Equine CD14 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Porcine/Equine CD14 Antibody

For research use only

Citations for Porcine/Equine CD14 Antibody

Customer Reviews for Porcine/Equine CD14 Antibody (1)

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  • Porcine/Equine CD14 Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: equine synovial tissue, Lymph node tissue and synovial tissue
    Species: Equine
    Verified Customer | Posted 05/09/2022
    Primary antibody was diluted 1:20-1:100. Secondary detection was performed with anti-mouse binding protein conjugated to HRP and subsequent incubation with DAB chromogen.
    Porcine/Equine CD14 Antibody MAB4597

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