Rat IL-1 beta /IL-1F2 Antibody Summary
Accession # Q63264
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutral-ization by Rat IL‑1 beta /IL‑1F2 Antibody. Recombinant Rat IL-1 beta /IL-1F2 (Catalog # 501-RL) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL-1 beta /IL-1F2 (4 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Rat IL-1 beta /IL-1F2 Monoclonal Antibody (Catalog # MAB501). The ND50 is typically 10-30 µg/mL.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-1 beta/IL-1F2
IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2, IL1B), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 26% amino acid (aa) identity in rat. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI. Intracellular cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. The 17 kDa molecular weight mature rat IL-1 beta shares 90% aa sequence identity with cotton rat and mouse and 67%-78% with canine, equine, feline, human, porcine, and rhesus macaque IL-1 beta. IL-1 beta functions in a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases.
Citations for Rat IL-1 beta /IL-1F2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 4
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Human mesenchymal stromal cells ameliorate complement induced inflammatory cascade and improve renal functions in a rat model of ischemia-reperfusion induced acute kidney injury
Authors: S Zilberman-, R Abu-Hamad, R Zarura, M Sova, Y Hachmo, M Stark, S Neuman, S Slavin, S Efrati
PLoS ONE, 2019;14(9):e0222354.
Sample Types: Whole Tissue
Applications: Fluorescense Microscopy
Aging in the Syrian hamster testis: Inflammatory-oxidative status and the impact of photoperiod
Authors: ME Matzkin, P Valchi, E Riviere, SP Rossi, YE Tavalieri, MMM de Toro, A Mayerhofer, A Bartke, RS Calandra, MB Frungieri
Exp. Gerontol., 2019;0(0):110649.
Sample Types: Cell Culture Supernates
Applications: Western Blot
Ischemic neurons prevent vascular regeneration of neural tissue by secreting semaphorin 3A.
Authors: Joyal JS, Sitaras N, Binet F, Rivera JC, Stahl A, Zaniolo K, Shao Z, Polosa A, Zhu T, Hamel D, Djavari M, Kunik D, Honore JC, Picard E, Zabeida A, Varma DR, Hickson G, Mancini J, Klagsbrun M, Costantino S, Beausejour C, Lachapelle P, Smith LE, Chemtob S, Sapieha P
Sample Types: Tissue Homogenates
Applications: Western Blot
Cytokine production by mouse myeloid dendritic cells in relation to differentiation and terminal maturation induced by lipopolysaccharide or CD40 ligation.
Authors: Morelli AE, Zahorchak AF, Larregina AT
Sample Types: Whole Cells
Applications: Flow Cytometry
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