Detects rat IL‑1 beta /IL‑1F2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross‑reactivity with recombinant human IL-1 alpha, recombinant mouse IL-1 alpha, and recombinant rat IL-1 alpha is observed.
Monoclonal Mouse IgG1 Clone # 38139
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant rat IL‑1 beta /IL‑1F2 Val117-Ser268 Accession # Q63264
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Immersion fixed rat splenocytes activated with LPS
Measured by its ability to neutralize IL‑1 beta /IL‑1F2-induced proliferation in the D10.G4.1 mouse helper T cell line. Symons, J. A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M. J. et al. (eds): IRL Press. 272. The Neutralization Dose (ND50) is typically 10-30 µg/mL in the presence of 4 ng/mL Recombinant Rat IL‑1 beta /IL‑1F2 and 1.25 µg/mL concanavalin A.
Please Note: Optimal dilutions should be determined by each laboratory for each application.
are available in the Technical Information section on our website.
Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutralization by Rat IL‑1 beta /IL‑1F2 Antibody.
Recombinant Rat IL‑1 beta /IL‑1F2 (Catalog # 501-RL) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL‑1 beta /IL‑1F2 (4 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Rat IL‑1 beta /IL‑1F2 Monoclonal Antibody (Catalog # MAB501). The ND50 is typically 10-30 µg/mL in the presence of concanavalin A (1.25 µg/mL).
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-1 beta/IL-1F2
IL-1 beta is a prototypical member of the IL-1 superfamily and is designated IL-1F2. The biological activity of IL-1 beta
is mediated by the heterodimeric receptor complex containing IL-1 R1
(IL-1 RI) and IL-1 R3 (IL-1 RAcP). IL-1 beta is a pleiotropic cytokine
involved in inflammation, development, growth and appetite.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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