Detects rat IL‑1 beta /IL‑1F2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross‑reactivity with recombinant human IL-1 alpha, recombinant mouse IL-1 alpha, and recombinant rat IL-1 alpha is observed.
Monoclonal Mouse IgG1 Clone # 38139
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant rat IL‑1 beta /IL‑1F2 Val117-Ser268 Accession # Q63264
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Immersion fixed rat splenocytes activated with LPS
Measured by its ability to neutralize IL‑1 beta /IL‑1F2-induced proliferation in the D10.G4.1 mouse helper T cell line. Symons, J. A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M. J. et al. (eds): IRL Press. 272. The Neutralization Dose (ND50) is typically 10-30 µg/mL in the presence of 4 ng/mL Recombinant Rat IL‑1 beta /IL‑1F2 and 1.25 µg/mL concanavalin A.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutralization by Rat IL‑1 beta /IL‑1F2 Antibody. Recombinant Rat IL‑1 beta /IL‑1F2 (Catalog # 501-RL) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL‑1 beta /IL‑1F2 (4 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Rat IL‑1 beta /IL‑1F2 Monoclonal Antibody (Catalog # MAB501). The ND50 is typically 10-30 µg/mL in the presence of concanavalin A (1.25 µg/mL).
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-1 beta/IL-1F2
IL-1 beta is a prototypical member of the IL-1 superfamily and is designated IL-1F2. The biological activity of IL-1 beta
is mediated by the heterodimeric receptor complex containing IL-1 R1
(IL-1 RI) and IL-1 R3 (IL-1 RAcP). IL-1 beta is a pleiotropic cytokine
involved in inflammation, development, growth and appetite.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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Ischemic neurons prevent vascular regeneration of neural tissue by secreting semaphorin 3A. Authors: Joyal JS, Sitaras N, Binet F, Rivera JC, Stahl A, Zaniolo K, Shao Z, Polosa A, Zhu T, Hamel D, Djavari M, Kunik D, Honore JC, Picard E, Zabeida A, Varma DR, Hickson G, Mancini J, Klagsbrun M, Costantino S, Beausejour C, Lachapelle P, Smith LE, Chemtob S, Sapieha P Blood, 2011;117(22):6024-35. Species: Mouse Sample Type: Tissue Homogenates Application: WB
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