Rat IL‑1 beta /IL‑1F2 Antibody

Name: Rat IL‑1 beta /IL‑1F2 Antibody
Brand: R&D Systems
SKU: MAB501
Price: 589 USD
Availability: InStock

R&D Systems | Catalog # MAB501

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Citations (10)

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
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Specific Notices
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Key Product Details

Species Reactivity

Validated:

Rat

Cited:

Mouse, Rat, Hamster

Applications

Validated:

Immunohistochemistry, Neutralization, Immunocytochemistry

Cited:

Western Blot, Flow Cytometry, Fluorescense Microscopy

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # 38139

View all IL-1 beta/IL-1F2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant rat IL‑1 beta /IL‑1F2
Val117-Ser268
Accession # Q63264

Specificity

Detects rat IL‑1 beta /IL‑1F2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross‑reactivity with recombinant human IL-1 alpha, recombinant mouse IL-1 alpha, and recombinant rat IL-1 alpha is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Rat IL‑1 beta /IL‑1F2 Antibody

Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutral-ization by Rat IL‑1 beta /IL‑1F2 Antibody.

Recombinant Rat IL-1 beta /IL-1F2 (Catalog # 501-RL) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL-1 beta /IL-1F2 (4 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Rat IL-1 beta /IL-1F2 Monoclonal Antibody (Catalog # MAB501). The ND₅₀ is typically 10-30 µg/mL.

Detection of IL‑1 beta /IL‑1F2 in Rat Splenocytes.

IL‑1 beta /IL‑1F2 was detected in Immersion fixed Rat Splenocytes cells using Mouse Anti-Rat IL‑1 beta /IL‑1F2 Monoclonal Antibody (Catalog # MAB501) at 10 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of IL‑1 beta /IL‑1F2 in Rat Intestine.

IL‑1 beta /IL‑1F2 was detected in perfusion fixed paraffin-embedded sections of rat intestine using Mouse Anti-Rat IL‑1 beta /IL‑1F2 Monoclonal Antibody (Catalog # MAB501) at 15 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of IL‑1 beta /IL‑1F2 in Rat Liver.

IL‑1 beta /IL‑1F2 was detected in perfusion fixed paraffin-embedded sections of rat liver using Mouse Anti-Rat IL‑1 beta /IL‑1F2 Monoclonal Antibody (Catalog # MAB501) at 15 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of IL‑1 beta /IL‑1F2 in Rat Stomach.

IL‑1 beta /IL‑1F2 was detected in perfusion fixed paraffin-embedded sections of rat stomach using Mouse Anti-Rat IL‑1 beta /IL‑1F2 Monoclonal Antibody (Catalog # MAB501) at 15 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

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Applications for Rat IL‑1 beta /IL‑1F2 Antibody

Application

Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed rat splenocytes

Immunohistochemistry

3-25 µg/mL
Sample: Perfusion fixed paraffin-embedded sections of rat intestine, liver, and stomach

Neutralization

Measured by its ability to neutralize IL‑1 beta /IL‑1F2-induced proliferation in the D10.G4.1 mouse helper T cell line. Symons, J. A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M. J. et al. (eds): IRL Press. 272. The Neutralization Dose (ND₅₀) is typically 10-30 µg/mL in the presence of 4 ng/mL Recombinant Rat IL‑1 beta /IL‑1F2.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: IL-1 beta/IL-1F2

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2, IL1B), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 26% amino acid (aa) identity in rat. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI. Intracellular cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. The 17 kDa molecular weight mature rat IL-1 beta shares 90% aa sequence identity with cotton rat and mouse and 67%-78% with canine, equine, feline, human, porcine, and rhesus macaque IL-1 beta. IL-1 beta functions in a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases.