Rat TIM-1/KIM-1/HAVCR Antibody

Catalog # Availability Size / Price Qty
AF3689
AF3689-SP
Rat TIM-1/KIM-1/HAVCR Antibody in Immunohistochemistry
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Citations (13)
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Rat TIM-1/KIM-1/HAVCR Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat TIM-1/KIM-1/HAVCR in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse (rm) TIM-1 is observed, and less than 1% cross-reactivity with recombinant human TIM-1, recombinant rat TIM-2, rmTIM-3, rmTIM-4, rmTIM-6, and rmTIM-7 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant rat TIM-1/KIM-1/HAVCR
Ser18-Val238
Accession # O54947
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Rat TIM‑1/KIM‑1/HAVCR (Catalog # 3689-TM)
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry TIM‑1/KIM‑1/HAVCR in Rat Kidney. View Larger

TIM‑1/KIM‑1/HAVCR in Rat Kidney. TIM-1/KIM-1/HAVCR was detected in perfusion fixed frozen sections of rat kidney using 15 µg/mL Rat TIM-1/KIM-1/HAVCR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3689) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TIM-1/KIM-1/HAVCR

KIM-1 (Kidney-injury molecule-1; also TIM-1 and HAVCR) is a 50-80 kDa, variably glycosylated, type I transmembrane glycoprotein that is a member of the TIM family of immunoglobulin superfamily molecules. This gene family is involved in the regulation of Th1 and Th2-cell-mediated immunity. In mouse, there are eight known TIM/KIM genes (# 1-8) vs. only three genes in human (# 1, 3, 4). It is unknown if the rat genome exactly parallels that of mouse. Rat KIM-1 is synthesized as a 307 amino acid (aa) precursor that contains a 21 aa signal sequence, a 214 aa extracellular domain (ECD), a 21 aa transmembrane segment and a 51 aa cytoplasmic domain. The ECD contains one V-type Ig-like domain and a mucin region characterized by multiple Thr-Ser-Pro motifs. The mucin region may undergo extensive O-linked glycosylation. The mouse KIM-1 gene is highly polymorphic and this may be reflected in rat. In human, TIM-1 is known to circulate as a soluble form. It undergoes constitutive cleavage by an undefined MMP, releasing an 85 kDa soluble molecule. A similar process has now been described in rat. The ECD of rat KIM-1 is 50% and 81% aa identical to human and mouse KIM-1 ECD, respectively. The only two reported ligands for KIM-1 are TIM-4 and the hepatitis A virus. However, others are believed to exist, and based on the ligand for TIM-3, one might be an S-type lectin. KIM-1 is found on CD4+ T cells and proximal renal tubular cells. KIM-1 ligation induces T cell proliferation and promotes cytokine production. In particular, it induces IL-4 production, and requires the KIM-1 cytoplasmic tyrosine phosphorylation motif. Alternatively, KIM-1 activation of TIM-4 induces cell cycle arrest.

Long Name
T Cell Immunoglobulin Mucin-1
Entrez Gene IDs
26762 (Human); 171283 (Mouse); 286934 (Rat); 102141332 (Cynomolgus Monkey)
Alternate Names
CD365; HAVCR1; HAVCR-1; HAVCRT cell immunoglobin domain and mucin domain protein 1; hepatitis A virus cellular receptor 1; Kidney injury molecule 1; KIM1; KIM-1; T-cell immunoglobulin and mucin domain-containing protein 1; TIM1; TIM-1; TIM-1TIM; TIM1TIMD-1; TIMD1T-cell membrane protein 1

Product Datasheets

Citations for Rat TIM-1/KIM-1/HAVCR Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. Canagliflozin, an SGLT2 inhibitor, normalizes renal susceptibility to type 1 cardiorenal syndrome through reduction of renal oxidative stress in diabetic rats
    Authors: Y Kimura, A Kuno, M Tanno, T Sato, K Ohno, S Shibata, K Nakata, H Sugawara, K Abe, Y Igaki, T Yano, T Miki, T Miura
    J Diabetes Investig, 2019;0(0):.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-P
  2. High fructose diet feeding accelerates diabetic nephropathy in Spontaneously Diabetic Torii (SDT) rats
    Authors: K Toyoda, Y Suzuki, K Muta, T Masuyama, K Kakimoto, A Kobayashi, T Shoda, S Sugai
    J Toxicol Sci, 2018;43(1):45-58.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-P
  3. Wnt4 is significantly upregulated during the early phases of cisplatin-induced acute kidney injury
    Authors: YX He, TT Diao, SM Song, CC Wang, Y Wang, CL Zhou, YB Bai, SS Yu, X Mi, XY Yang, QJ Wei, B Li
    Sci Rep, 2018;8(1):10555.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  4. Osteopontin deficiency ameliorates Alport pathology by preventing tubular metabolic deficits
    Authors: W Ding, K Yousefi, S Goncalves, BJ Goldstein, AL Sabater, A Kloosterbo, P Ritter, G Lambert, AJ Mendez, LA Shehadeh
    JCI Insight, 2018;3(6):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  5. The 6-hydroxychromanol derivative SUL-109 ameliorates renal injury after deep hypothermia and rewarming in rats
    Authors: PC Vogelaar, M Roorda, EL de Vrij, MC Houwertjes, M Goris, H Bouma, AC van der Gr, G Krenning, RH Henning
    Nephrol. Dial. Transplant., 2018;0(0):.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  6. Combined therapy with melatonin and exendin-4 effectively attenuated the deterioration of renal function in rat cardiorenal syndrome
    Authors: KH Chen, CH Chen, CG Wallace, YT Chen, CC Yang, PH Sung, HJ Chiang, YL Chen, S Chua, HK Yip, JT Cheng
    Am J Transl Res, 2017;9(2):214-229.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-P
  7. Combination therapy of exendin-4 and allogenic adipose-derived mesenchymal stem cell preserved renal function in a chronic kidney disease and sepsis syndrome setting in rats
    Authors: CH Chen, BC Cheng, KH Chen, PL Shao, PH Sung, HJ Chiang, CC Yang, KC Lin, CK Sun, JJ Sheu, HW Chang, MS Lee, HK Yip
    Oncotarget, 2017;8(59):100002-100020.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-P
  8. Endocytosis of Albumin Induces Matrix Metalloproteinase-9 by Activating the ERK Signaling Pathway in Renal Tubule Epithelial Cells
    Authors: X Chen, A Cobbs, J George, A Chima, F Tuyishime, X Zhao
    Int J Mol Sci, 2017;18(8):.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  9. An Atherogenic Paigen-Diet Aggravates Nephropathy in Type 2 Diabetic OLETF Rats.
    Authors: Nozako M, Koyama T, Nagano C, Sato M, Matsumoto S, Mitani K, Yasufuku R, Kohashi M, Yoshikawa T
    PLoS ONE, 2015;10(11):e0143979.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-P
  10. Altered expression profile of renal alpha(1D)-adrenergic receptor in diabetes and its modulation by PPAR agonists.
    Authors: Zhao X, Zhang Y, Leander M, Li L, Wang G, Emmett N
    J Diabetes Res, 2014;2014(0):725634.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  11. The uremic toxin adsorbent AST-120 abrogates cardiorenal injury following myocardial infarction.
    Authors: Lekawanvijit S, Kumfu S, Wang B, Manabe M, Nishijima F, Kelly D, Krum H, Kompa A
    PLoS ONE, 2013;8(12):e83687.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  12. Urinary levels of regenerating islet-derived protein III beta and gelsolin differentiate gentamicin from cisplatin-induced acute kidney injury in rats.
    Authors: Ferreira L, Quiros Y, Sancho-Martinez SM, Garcia-Sanchez O, Raposo C, Lopez-Novoa JM, Gonzalez-Buitrago JM, Lopez-Hernandez FJ
    Kidney Int., 2011;79(5):518-28.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  13. Sub-nephrotoxic doses of gentamicin predispose animals to developing acute kidney injury and to excrete ganglioside M2 activator protein.
    Authors: Quiros Y, Ferreira L, Sancho-Martinez SM, Gonzalez-Buitrago JM, Lopez-Novoa JM, Lopez-Hernandez FJ
    Kidney Int., 2010;78(10):1006-15.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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