Rat TIM-1/KIM-1/HAVCR Antibody

Catalog # Availability Size / Price Qty
TIM‑1/KIM‑1/HAVCR in Rat Kidney.
1 Image
Product Details
Citations (19)
Supplemental Products

Rat TIM-1/KIM-1/HAVCR Antibody Summary

Species Reactivity
Detects rat TIM-1/KIM-1/HAVCR in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse (rm) TIM-1 is observed, and less than 1% cross-reactivity with recombinant human TIM-1, recombinant rat TIM-2, rmTIM-3, rmTIM-4, rmTIM-6, and rmTIM-7 is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
Mouse myeloma cell line NS0-derived recombinant rat TIM-1/KIM-1/HAVCR
Accession # O54947
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Rat TIM‑1/KIM‑1/HAVCR (Catalog # 3689-TM)
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry TIM-1/KIM-1/HAVCR antibody in Rat Kidney by Immunohistochemistry (IHC-Fr). View Larger

TIM‑1/KIM‑1/HAVCR in Rat Kidney. TIM-1/KIM-1/HAVCR was detected in perfusion fixed frozen sections of rat kidney using 15 µg/mL Rat TIM-1/KIM-1/HAVCR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3689) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TIM-1/KIM-1/HAVCR

KIM-1 (Kidney-injury molecule-1; also TIM-1 and HAVCR) is a 50-80 kDa, variably glycosylated, type I transmembrane glycoprotein that is a member of the TIM family of immunoglobulin superfamily molecules. This gene family is involved in the regulation of Th1 and Th2-cell-mediated immunity. In mouse, there are eight known TIM/KIM genes (# 1-8) vs. only three genes in human (# 1, 3, 4). It is unknown if the rat genome exactly parallels that of mouse. Rat KIM-1 is synthesized as a 307 amino acid (aa) precursor that contains a 21 aa signal sequence, a 214 aa extracellular domain (ECD), a 21 aa transmembrane segment and a 51 aa cytoplasmic domain. The ECD contains one V-type Ig-like domain and a mucin region characterized by multiple Thr-Ser-Pro motifs. The mucin region may undergo extensive O-linked glycosylation. The mouse KIM-1 gene is highly polymorphic and this may be reflected in rat. In human, TIM-1 is known to circulate as a soluble form. It undergoes constitutive cleavage by an undefined MMP, releasing an 85 kDa soluble molecule. A similar process has now been described in rat. The ECD of rat KIM-1 is 50% and 81% aa identical to human and mouse KIM-1 ECD, respectively. The only two reported ligands for KIM-1 are TIM-4 and the hepatitis A virus. However, others are believed to exist, and based on the ligand for TIM-3, one might be an S-type lectin. KIM-1 is found on CD4+ T cells and proximal renal tubular cells. KIM-1 ligation induces T cell proliferation and promotes cytokine production. In particular, it induces IL-4 production, and requires the KIM-1 cytoplasmic tyrosine phosphorylation motif. Alternatively, KIM-1 activation of TIM-4 induces cell cycle arrest.

Long Name
T Cell Immunoglobulin Mucin-1
Entrez Gene IDs
26762 (Human); 171283 (Mouse); 286934 (Rat); 102141332 (Cynomolgus Monkey)
Alternate Names
CD365; HAVCR1; HAVCR-1; HAVCRT cell immunoglobin domain and mucin domain protein 1; hepatitis A virus cellular receptor 1; Kidney injury molecule 1; KIM1; KIM-1; T-cell immunoglobulin and mucin domain-containing protein 1; TIM1; TIM-1; TIM-1TIM; TIM1TIMD-1; TIMD1T-cell membrane protein 1

Product Datasheets

You must select a language.


Product Specific Notices

This product is covered by one or more of the following US Patents 7,300,652; 7,041,290; 6,664,385 and other US and foreign patents pending or issued.

Citations for Rat TIM-1/KIM-1/HAVCR Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

19 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. Accelerated lysine metabolism conveys kidney protection in salt-sensitive hypertension
    Authors: MM Rinschen, O Palygin, A El-Meanawy, X Domingo-Al, A Palermo, LV Dissanayak, D Golosova, MA Schafroth, C Guijas, F Demir, J Jaegers, ML Gliozzi, J Xue, M Hoehne, T Benzing, BP Kok, E Saez, M Bleich, N Himmerkus, OA Weisz, BF Cravatt, M Krüger, HP Benton, G Siuzdak, A Staruschen
    Nature Communications, 2022;13(1):4099.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  2. Nephrotoxicity evaluation and proteomic analysis in kidneys of rats exposed to thioacetamide
    Authors: JY Lim, WW Jung, W Kim, KS Moon, D Sul
    Scientific Reports, 2022;12(1):6837.
    Species: Rat
    Sample Types: Tissue Lysates
    Applications: Western Blot
  3. Using Green Biosynthesized Lycopene-Coated Selenium Nanoparticles to Rescue Renal Damage in Glycerol-Induced Acute Kidney Injury in Rats
    Authors: A Al-Brakati, KF Alsharif, KJ Alzahrani, S Kabrah, O Al-Amer, AA Oyouni, OA Habotta, MS Lokman, AA Bauomy, RB Kassab, AE Abdel Mone
    International Journal of Nanomedicine, 2021;16(0):4335-4349.
    Species: Rat
    Sample Types: Plasma
    Applications: ELISA Capture
  4. Empagliflozin attenuates acute kidney injury after myocardial infarction in diabetic rats
    Authors: A Kuno, Y Kimura, M Mizuno, H Oshima, T Sato, N Moniwa, M Tanaka, T Yano, M Tanno, T Miki, T Miura
    Sci Rep, 2020;10(1):7238.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  5. Methionine abrogates the renoprotective effect of a low-protein diet against diabetic kidney disease in obese rats with type 2 diabetes
    Authors: M Kitada, Y Ogura, I Monno, J Xu, D Koya
    Aging (Albany NY), 2020;12(5):4489-4505.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  6. Distinct inactivated bacterial-based immune modulators vary in their therapeutic efficacies for treating disease based on the organ site of pathology
    Authors: S Kalyan, M Bazett, HP Sham, M Bosiljcic, B Luk, S Dhanji, AM Costa, SWY Wong, MG Netea, DW Mullins, H Gunn
    Sci Rep, 2020;10(1):5901.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  7. Canagliflozin, an SGLT2 inhibitor, normalizes renal susceptibility to type 1 cardiorenal syndrome through reduction of renal oxidative stress in diabetic rats
    Authors: Y Kimura, A Kuno, M Tanno, T Sato, K Ohno, S Shibata, K Nakata, H Sugawara, K Abe, Y Igaki, T Yano, T Miki, T Miura
    J Diabetes Investig, 2019;0(0):.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-P
  8. High fructose diet feeding accelerates diabetic nephropathy in Spontaneously Diabetic Torii (SDT) rats
    Authors: K Toyoda, Y Suzuki, K Muta, T Masuyama, K Kakimoto, A Kobayashi, T Shoda, S Sugai
    J Toxicol Sci, 2018;43(1):45-58.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-P
  9. Osteopontin deficiency ameliorates Alport pathology by preventing tubular metabolic deficits
    Authors: W Ding, K Yousefi, S Goncalves, BJ Goldstein, AL Sabater, A Kloosterbo, P Ritter, G Lambert, AJ Mendez, LA Shehadeh
    JCI Insight, 2018;3(6):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  10. Wnt4 is significantly upregulated during the early phases of cisplatin-induced acute kidney injury
    Authors: YX He, TT Diao, SM Song, CC Wang, Y Wang, CL Zhou, YB Bai, SS Yu, X Mi, XY Yang, QJ Wei, B Li
    Sci Rep, 2018;8(1):10555.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  11. The 6-hydroxychromanol derivative SUL-109 ameliorates renal injury after deep hypothermia and rewarming in rats
    Authors: PC Vogelaar, M Roorda, EL de Vrij, MC Houwertjes, M Goris, H Bouma, AC van der Gr, G Krenning, RH Henning
    Nephrol. Dial. Transplant., 2018;0(0):.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  12. Combined therapy with melatonin and exendin-4 effectively attenuated the deterioration of renal function in rat cardiorenal syndrome
    Authors: KH Chen, CH Chen, CG Wallace, YT Chen, CC Yang, PH Sung, HJ Chiang, YL Chen, S Chua, HK Yip, JT Cheng
    Am J Transl Res, 2017;9(2):214-229.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-P
  13. Combination therapy of exendin-4 and allogenic adipose-derived mesenchymal stem cell preserved renal function in a chronic kidney disease and sepsis syndrome setting in rats
    Authors: CH Chen, BC Cheng, KH Chen, PL Shao, PH Sung, HJ Chiang, CC Yang, KC Lin, CK Sun, JJ Sheu, HW Chang, MS Lee, HK Yip
    Oncotarget, 2017;8(59):100002-100020.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-P
  14. Endocytosis of Albumin Induces Matrix Metalloproteinase-9 by Activating the ERK Signaling Pathway in Renal Tubule Epithelial Cells
    Authors: X Chen, A Cobbs, J George, A Chima, F Tuyishime, X Zhao
    Int J Mol Sci, 2017;18(8):.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  15. An Atherogenic Paigen-Diet Aggravates Nephropathy in Type 2 Diabetic OLETF Rats.
    Authors: Nozako M, Koyama T, Nagano C, Sato M, Matsumoto S, Mitani K, Yasufuku R, Kohashi M, Yoshikawa T
    PLoS ONE, 2015;10(11):e0143979.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-P
  16. Altered expression profile of renal alpha(1D)-adrenergic receptor in diabetes and its modulation by PPAR agonists.
    Authors: Zhao X, Zhang Y, Leander M, Li L, Wang G, Emmett N
    J Diabetes Res, 2014;2014(0):725634.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  17. The uremic toxin adsorbent AST-120 abrogates cardiorenal injury following myocardial infarction.
    Authors: Lekawanvijit S, Kumfu S, Wang B, Manabe M, Nishijima F, Kelly D, Krum H, Kompa A
    PLoS ONE, 2013;8(12):e83687.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  18. Urinary levels of regenerating islet-derived protein III beta and gelsolin differentiate gentamicin from cisplatin-induced acute kidney injury in rats.
    Authors: Ferreira L, Quiros Y, Sancho-Martinez SM, Garcia-Sanchez O, Raposo C, Lopez-Novoa JM, Gonzalez-Buitrago JM, Lopez-Hernandez FJ
    Kidney Int., 2011;79(5):518-28.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  19. Sub-nephrotoxic doses of gentamicin predispose animals to developing acute kidney injury and to excrete ganglioside M2 activator protein.
    Authors: Quiros Y, Ferreira L, Sancho-Martinez SM, Gonzalez-Buitrago JM, Lopez-Novoa JM, Lopez-Hernandez FJ
    Kidney Int., 2010;78(10):1006-15.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot


No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Rat TIM-1/KIM-1/HAVCR Antibody

There are currently no reviews for this product. Be the first to review Rat TIM-1/KIM-1/HAVCR Antibody and earn rewards!

Have you used Rat TIM-1/KIM-1/HAVCR Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review