Bovine IL-2 Antibody

  
  • Species Reactivity
    Bovine
  • Specificity
    Detects bovine IL-2 in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant human IL-2, recombinant mouse IL-2, recombinant rat IL-2, recombinant feline IL-2, recombinant canine IL-2, recombinant equine IL-2, recombinant cotton rat IL-2, and recombinant porcine IL-2 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant bovine IL‑2
    Ala21-Thr155
    Accession # P05016
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Bovine IL‑2 (Catalog # 2465-BL)
  • Immunocytochemistry
    5-15 µg/mL
    See below
    • Bovine IL-2 Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    0.2-0.8 µg/mL 
    Bovine IL‑2 Antibody (Catalog # AF2465)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Bovine IL‑2 Biotinylated Antibody (Catalog # BAF2465)
  • ELISA Standard
     
    Recombinant Bovine IL-2 Protein (Catalog # 2465-BL)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
IL‑2 in Bovine PBMCs. IL‑2 was detected in immersion fixed bovine peripheral blood mononuclear cells (PBMCs) using Goat Anti-Bovine IL‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2465) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-2

Bovine Interleukin-2 (IL-2) is a 15 kDa, alpha -helical, single chain, potentially glycosylated polypeptide that has potent stimulatory activity for antigen-activated T cells (1‑5). The molecule is synthesized as a 155 amino acid (aa) precursor that contains a 20 aa signal peptide plus a 135 aa mature segment that is possibly O‑glycosylated (4, 5). The mature region has multiple alpha -helices and one intrachain disulfide bond. Mature bovine IL-2 is 64%, 60%, 49%, 50%, 72%, 63% and 67% to mature human, canine, mouse, rat, porcine, equine, and feline IL-2, respectively. Mammalian cells known to express IL-2 include CD4+ and CD8+ T cells, visceral smooth muscle cells, eosinophils, gamma δ T cells, B cells and dendritic cells. The receptor for IL-2 is complex and consists of three distinct subunits in varying combinations (6, 7). Two of these are ligand-binding and are termed IL-2 R alpha and IL-2 R beta. IL-2 R alpha is 55 kDa and binds IL-2 with low affinity. IL-2 R beta is 75 kDa and binds IL-2 with intermediate affinity. Signal transduction is performed by both IL-2 R beta and a 64 kDa common gamma chain ( gamma c). This signal transducing common gamma chain does not bind IL-2, but does heterodimerize with IL-2 R beta to form a functional IL-2 receptor. The complex heterotrimeric alpha -beta -gamma c receptor may arise from IL-2 binding to preformed R alpha -R beta  complexes (8). Functionally, IL-2 is best known for its autocrine and paracrine activity on T cells. It drives resting T cells into active G1, inducing IL-2 and IL-2 R alpha synthesis and cell proliferation (7). It also promotes Fas-induced death of naïve CD4+ T cells, while having minimal effect on activated CD4+ memory lymphocytes. Finally, IL-2 seems to play a central role in the expansion and maintenance of CD4+ CD25+ regulatory T cells. Thus, IL-2 may be a key cytokine in the natural suppression of autoimmunity (9, 10).

  • References:
    1. Smith, K.A. (1992) Curr. Opin. Immunol. 4:271.
    2. Smith, K.A. (1988) Science 240:1169.
    3. Waldmann, T.A. et al. (2001) Immunity 14:105.
    4. Cerretti, D.P. et al. (1986) Proc. Natl. Acad. Sci. USA 83:3223.
    5. Reeves, R. et al. (1986) Proc. Natl. Acad. Sci. USA 83:3228.
    6. Ellery, J.M. and P.J. Nicholls (2002) Cytokine Growth Factor Rev. 13:27.
    7. Nelson, B.H. and D.M. Willerford (1998) Adv. Immunol. 70:1.
    8. Liparoto, S.F. et al. (2002) Biochemistry 41:2543.
    9. Jaleco, S. et al. (2003) J. Immunol. 171:61.
    10. Malek, T.R. (2003) J. Leukoc. Biol. 74:961.
  • Long Name:
    Interleukin 2
  • Entrez Gene IDs:
    3558 (Human); 16183 (Mouse); 116562 (Rat); 396868 (Porcine); 280822 (Bovine); 403989 (Canine); 100034204 (Equine); 751114 (Feline); 100302458 (Rabbit)
  • Alternate Names:
    aldesleukin; IL2; IL-2; IL-2lymphokine; interleukin 2; interleukin-2; involved in regulation of T-cell clonal expansion; T cell growth factor; T-cell growth factor; TCGF
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