Key Product Details

Species Reactivity

Validated:

Bovine

Cited:

Bovine

Applications

Validated:

Western Blot, ELISA Capture (Matched Antibody Pair), Immunocytochemistry

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all TNF-alpha Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant bovine TNF-alpha
Leu78-Leu234
Accession # Q06599

Specificity

Detects bovine TNF-alpha in ELISAs and Western blots. In sandwich immunoassays, less than 0.3% cross-reactivity with recombinant human TNF-alpha, recombinant mouse TNF‑ alpha, recombinant rat TNF‑ alpha, recombinant feline TNF‑ alpha, recombinant equine TNF‑ alpha, recombinant porcine TNF‑ alpha, recombinant cotton rat TNF-alpha, and recombinant rhesus macaque TNF-alpha is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Bovine TNF‑ alpha Antibody

TNF-a antibody in Bovine PBMCs by Immunocytochemistry (ICC).

TNF‑ alpha in Bovine PBMCs.

TNF-a was detected in immersion fixed bovine peripheral blood mononuclear cells (PBMCs) using Goat Anti-Bovine TNF-a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2279) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Bovine TNF‑ alpha Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed bovine peripheral blood mononuclear cells (PBMCs)

Western Blot

0.1 µg/mL
Sample: Recombinant Bovine TNF‑ alpha (Catalog # 2279-BT)

Bovine TNF-alpha Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 0.2-0.8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Bovine TNF‑ alpha Biotinylated Antibody (Catalog # BAF2279)
  • Standard: Recombinant Bovine TNF-alpha Protein (Catalog # 2279-BT)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using AF2279 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TNF-alpha

Tumor necrosis factor alpha (TNF-alpha, also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, apoptosis, and immune system development. TNF-alpha is produced by a wide variety of immune and epithelial cell types (1, 2). Bovine TNF-alpha consisits of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 178 aa extracellular domain (ECD) (3). Within the ECD, bovine TNF-alpha shares 64%-83% sequence identity with canine, cotton rat, equine, feline, human, mouse, porcine, rat, and rhesus TNF-alpha. The 26 kDa type 2 transmembrane protein is assembled intracellularly to form a noncovalently linked homotrimer (4). Ligation of this complex induces reverse signaling that promotes lymphocyte costimulation but diminishes monocyte responsiveness (5). Cleavage of membrane bound TNF-alpha by TACE/ADAM17 releases a 55 kDa soluble trimeric form of TNF-alpha (6, 7). TNF-alpha trimers bind the ubiquitous TNF RI and the hematopoietic cell-restricted TNF RII, both of which are also expressed as homotrimers (1, 8). TNF-alpha regulates lymphoid tissue development through control of apoptosis (2). It also promotes inflammatory responses by inducing the activation of vascular endothelial cells and macrophages (2). TNF-alpha is a key cytokine in several inflammatory disorders (9). It contributes to the development of type 2 diabetes through its effects on insulin resistance and fatty acid metabolism (10, 11).

References

  1. Idriss, H.T. and J.H. Naismith (2000) Microsc. Res. Tech. 50:184.
  2. Hehlgans, T. and K. Pfeffer (2005) Immunology 115:1.
  3. Cludts, I. et al. (1993) Cytokine 5:336.
  4. Tang, P. et al. (1996) Biochemistry 35:8216.
  5. Eissner G. et al. (2004) Cytokine Growth Factor Rev. 15:353.
  6. Black, R.A. et al. (1997) Nature 385:729.
  7. Moss, M.L. et al. (1997) Nature 385:733.
  8. Loetscher, H. et al. (1991) J. Biol. Chem. 266:18324.
  9. Clark, I.A. (2007) Cytokine Growth Factor Rev. 18:335.
  10. Romanatto, T. et al. (2007) Peptides 28:1050.
  11. Hector, J. et al. (2007) Horm. Metab. Res. 39:250.

Long Name

Tumor Necrosis Factor alpha

Alternate Names

Cachetin, DIF, TNF, TNF-A, TNFA, TNFalpha, TNFG1F, TNFSF1A, TNFSF2

Entrez Gene IDs

7124 (Human); 21926 (Mouse); 24835 (Rat); 397086 (Porcine); 280943 (Bovine); 403922 (Canine); 102139631 (Cynomolgus Monkey); 100033834 (Equine); 493755 (Feline); 100009088 (Rabbit)

Gene Symbol

TNF

UniProt

Q06599

Additional TNF-alpha Products

Product Documents for Bovine TNF‑ alpha Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Bovine TNF‑ alpha Antibody

For research use only

Citations for Bovine TNF‑ alpha Antibody

Customer Reviews for Bovine TNF‑ alpha Antibody (1)

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  • Bovine TNF-alpha Antibody
    Name: Katie Myers
    Application: ELISA
    Sample Tested: Serum
    Species: Amphibian and Bovine
    Verified Customer | Posted 11/14/2022
    This antibody worked well as both capture and detection in our assay.
    Bovine TNF‑ alpha Antibody AF2279

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Protocols

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