Quantikine® HS Colorimetric Sandwich ELISAs, High Sensitivity

Quantikine HS ELISA Kits are complete assays generally used where very low levels of the target protein are expected. These kits use an amplification system* in which an alkaline phosphatase-based amplification leads to the formation of a colored product.


  • Detect femtogram levels of protein
  • Complete ready to use kits
  • Exhaustively tested for superior quality and reproducibility
  • Detailed protocol booklets include inter- and intra-assay precision, recovery, linearity, sensitivity, and sample value data
  • Colorimetric detection

Assay Principle

View Full Assay Principle

Kit Contents

Kit contents may vary slightly. Please refer to product-specific inserts for a complete list of contents for each individual assay.

  • Pre-coated 96-well Microplate
  • Conjugated Detection Antibody
  • Calibrated Immunoassay Standard
  • Assay Diluent
  • Calibrator Diluent(s)
  • Wash Buffer
  • Substrate and Substrate Diluent
  • Amplifier and Amplifier Diluent
  • Stop Solution
  • Plate Sealers

*The Quantikine HS Human G-CSF ELISA Kit does not utilize this amplification system, rather it uses the substrate tetramethylbenzidine (TMB) for color development. Please see the appropriate Quantikine ELISA Assay Principle.