Human BMP-7 Antibody

(5 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human BMP-7 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human (rh) BMP-6 is observed and no cross-reactivity with rhBMP-2, -3, -4, -5, or -8 is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 164311
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant human BMP‑7
    Ser293-His431
    Accession # P18075
  • Formulation
    Supplied as a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human BMP‑7 (Catalog # 354-BP) under non-reducing conditions only
  • Immunohistochemistry
    8-25 µg/mL
    See below
  • Neutralization
    Measured by its ability to neutralize BMP‑7-induced alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. Erlacher, L. et al. (1998) J. Bone Miner. Res. 13:383. The Neutralization Dose (ND50) is typically 1.5-6.0 µg/mL in the presence of 1 µg/mL Recombinant Human BMP‑7 and 50 µg/mL L-ascorbic acid.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples

Alkaline Phosphatase Production Induced by BMP‑7 and Neutralization by Human BMP‑7 Antibody. Recombinant Human BMP‑7 (Catalog #
354‑BP) induces alkaline phosphatase production in the the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Human BMP‑7 (1 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human BMP‑7 Monoclonal Antibody (Catalog # MAB3541). The ND50 is typically 1.5-6.0 µg/mL in the presence of L-ascorbic acid (50 µg/mL).

BMP‑7 in Human Kidney. BMP‑7 was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human BMP‑7 Monoclonal Antibody (Catalog # MAB3541) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Preparation and Storage
  • Shipping
    The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C, as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after opening.
    • 6 months, -20 to -70 °C under sterile conditions after opening.
Background: BMP-7
Bone morphogenetic protein 7 (BMP-7), also known as osteogenic protein 1 (OP-1), is a widely expressed TGF-beta superfamily member with important functions during embryogenesis, in the adult, and in disease (1, 2). Human BMP-7 is synthesized with a 29 amino acid (aa) signal sequence, a 263 aa propeptide, and a 139 aa growth factor domain (3, 4). The growth factor domain of human BMP-7 shares 98% aa sequence identity with mouse and rat BMP-7. The BMP-7 propeptide is cleaved intracellularly but often remains associated with the mature C-terminus. Based on in vivo and in vitro studies, BMP-7 has the potential to be secreted as a disulfide‑linked mature homodimer, or particularly as a heteromeric complex that consists of two propeptides noncovalently associated with a mature disulfide‑linked homodimer (5, 6). The presence of the propeptides in BMP-7 appears to stabilize the molecule and provide a docking mechanism for extracellular storage on molecules such as fibrillin-1 and -2 (5, 6). The propeptides themselves do not impart latency to the complex. BMP-7 binding to type II receptors rapidly displaces the prodomain:mature molecule interaction and has no effect on activity. But it is suggested that immobilized BMP-7 (via prodomain:fibrillin) is inactive, allowing for possible long-term storage of the molecule (6). BMP-7 interacts with the type 2 receptors Activin RIIA, Activin RIIB, and BMPR-II and the type 1 receptors Activin RIA, BMPR-IA, and BMPR-IB (2, 6). BMP-7 may also be processed into a disulfide‑linked heterodimer with either BMP-2 or BMP-4. Such complexes may show increased potency and range of activity compared to BMP-7 homodimers (7‑9). BMP-7 plays a role in a variety of organ systems. It promotes new bone formation and nephron development (10, 11), inhibits the branching of prostate epithelium (12), and antagonizes epithelial-mesenchymal transition (EMT) (13‑15). In pathological conditions, BMP-7 inhibits tumor growth and metastasis (14), ameliorates fibrotic damage in nephritis (13), and promotes neuroregeneration following brain ischemia (16).
  • References:
    1. Chen, D. et al. (2004) Growth Factors 22:233.
    2. Kishigami, S. and Y. Mishina (2005) Cytokine Growth Factor Rev. 16:265.
    3. Ozkaynak, E. et al. (1990) EMBO J. 9:2085.
    4. Celeste, A.J. et al. (1990) Proc. Natl. Acad. Sci. 87:9843.
    5. Gregory, K.E. et al. (2005) J. Biol. Chem. 280:27970.
    6. Sengle, G. et al. (2008) J. Mol. Biol. 381:1025.
    7. Israel, D.I. et al. (1996) Growth Factors 13:291.
    8. Aono, A. et al. (1995) Biochem. Biophys. Res. Commun. 210:670.
    9. Nishimatsu, S. and G.H. Thomsen (1998) Mech. Dev. 74:75.
    10. Sampath, T.K. et al. (1992) J. Biol. Chem. 267:20352.
    11. Kazama, I. et al. (2008) J. Am. Soc. Nephrol. 19:2181.
    12. Grishina, I.B. et al. (2005) Dev. Biol. 288:334.
    13. Zeisberg, M. et al. (2003) Nat. Med. 9:964.
    14. Buijs, J.T. et al. (2007) Am. J. Pathol. 171:1047.
    15. Yu, M.-A. et al. (2009) J. Am. Soc. Nephrol. 20:567.
    16. Chou, J. et al. (2006) J. Neurol. Sci. 240:21.
  • Long Name:
    Bone Morphogenetic Protein 7
  • Entrez Gene IDs:
    655 (Human); 12162 (Mouse); 85272 (Rat)
  • Alternate Names:
    BMP7; BMP-7; bone morphogenetic protein 7; Eptotermin alfa; OP-1; OP-1OP1; Osteogenic protein 1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Species
Applications
Sample Type
  1. Regulation of retinal progenitor cell differentiation by bone morphogenetic protein 4 is mediated by the smad/id cascade.
    Authors: Du Y, Xiao Q, Yip HK
    Invest. Ophthalmol. Vis. Sci., 2010;51(7):3764-73.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  2. A new model for growth factor activation: type II receptors compete with the prodomain for BMP-7.
    Authors: Sengle G, Ono RN, Lyons KM, Bachinger HP, Sakai LY
    J. Mol. Biol., 2008;381(4):1025-39.
    Species: Human
    Sample Type: Recombinant Protein
    Application: ELISA Development
  3. Targeting of bone morphogenetic protein growth factor complexes to fibrillin.
    Authors: Sengle G, Charbonneau NL, Ono RN, Sasaki T, Alvarez J, Keene DR, Bachinger HP, Sakai LY
    J. Biol. Chem., 2008;283(20):13874-88.
    Species: Human
    Sample Type: Recombinant Protein
    Application: WB
  4. Aggressive melanoma cells escape from BMP7-mediated autocrine growth inhibition through coordinated Noggin upregulation.
    Authors: Hsu MY, Rovinsky SA, Lai CY, Qasem S, Liu X, How J, Engelhardt JF, Murphy GF
    Lab. Invest., 2008;88(8):842-55.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  5. Combinatorial gene therapy for bone regeneration: cooperative interactions between adenovirus vectors expressing bone morphogenetic proteins 2, 4, and 7.
    Authors: Zhao M, Zhao Z, Koh JT, Jin T, Franceschi RT
    J. Cell. Biochem., 2005;95(1):1-16.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: IP
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