Human CD25/IL-2 R alpha Antibody

(3 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human CD25/IL-2 R alpha in ELISAs and Western blots. In Western blots, detects recombinant rat CD25/IL-2 R alpha but no cross-reactivity with recombinant mouse CD25/IL-2 R alpha. In sandwich immunoassays, this antibody does not cross-react or interfere with recombinant human (rh) IL-1 RI, rhIL-1 RII, rhIL-2 R beta, rhIL-3 R alpha, rhIL-4 R, rhIL-5 R alpha, rhIL-5 R beta, rhIL-6 R, or rhgp130.
  • Source
    Monoclonal Mouse IgG1 Clone # 24204
  • Purification
    Protein A or G purified from ascites
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD25/IL-2 R alpha
    Glu22-Cys213
    Accession # P01589
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunohistochemistry
    8-25 µg/mL
    See below
  • Immunocytochemistry
    8-25 µg/mL
    See below
    • Human CD25/IL-2 R alpha Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Human CD25/IL-2 R alpha Antibody (Catalog # MAB623)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Human CD25/IL-2 R alpha Biotinylated Antibody (Catalog # BAF223)
  • ELISA Standard
     
    Recombinant Human CD25/IL-2 R alpha Protein (Catalog # 223-2A)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Recombinant Human and Rat CD25/IL-2 R alpha by Western Blot. Western blot shows 25 ng of Recombinant Human CD25/IL-2 R alpha (Catalog # 223-2A), Recombinant Mouse CD25/IL-2 R alpha (Catalog # 2438-RM) and Recombinant Rat CD25/IL-2 R alpha Fc Chimera (Catalog # 5156-RM). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human CD25/IL-2 R alpha Monoclonal Antibody (Catalog # MAB623) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for CD25/IL-2 R alpha at approximately 30-250 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 3.
Immunocytochemistry
CD25/IL‑2 R alpha in Human PBMCs. CD25/IL‑2 R alpha was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with PHA using Mouse Anti-Human CD25/IL‑2 R alpha Monoclonal Antibody (Catalog # MAB623) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). Upper panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Immunohistochemistry
CD25/IL‑2 R alpha in Human Lymph Node. CD25/IL‑2 R alpha was detected in immersion fixed frozen sections of human lymph node using 15 µg/mL Mouse Anti-Human CD25/IL‑2 R alpha Monoclonal Antibody (Catalog # MAB623) overnight at 4 °C. Tissue was stained (red) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD25/IL-2 R alpha
IL-2 R alpha is a type I transmembrane protein that associates with IL-2 R beta and the common gamma chain to form a high affinity heterotrimeric receptor complex for IL-2. A soluble form of IL-2 R alpha has been detected in serum.
  • Long Name:
    Interleukin 2 Receptor alpha
  • Entrez Gene IDs:
    3559 (Human); 16184 (Mouse); 25704 (Rat); 403870 (Canine)
  • Alternate Names:
    CD25 antigen; CD25; IDDM10; IL-2 R alpha; IL-2 receptor subunit alpha; IL2R alpha; IL-2R subunit alpha; IL2R; IL2RA; IL-2Ra; IL-2-RA; IL2-RA; interleukin 2 receptor, alpha; interleukin-2 receptor subunit alpha; p55; TAC antigen; TCGFR
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Applications
Sample Type
  1. Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
    Authors: Hu H, Kwun J, Aizenstein BD, Knechtle SJ
    Transplantation, 2009;87(12):1814-20.
    Species: Human
    Sample Type: Urine
    Application: Antibody Array Development
  2. Imbalance in the peritoneal levels of interleukin 1 and its decoy inhibitory receptor type II in endometriosis women with infertility and pelvic pain.
    Authors: Akoum A, Al-Akoum M, Lemay A, Maheux R, Leboeuf M
    Fertil. Steril., 2007;89(6):1618-24.
    Species: Human
    Sample Type: Peritoneal Fluid
    Application: ELISA Development
  3. Induction of lysosomal and plasma membrane-bound sialidases in human T-cells via T-cell receptor.
    Authors: Wang P, Zhang J, Bian H, Wu P, Kuvelkar R, Kung TT, Crawley Y, Egan RW, Billah MM
    Biochem. J., 2004;380(0):425-33.
    Species: Human
    Sample Type:
    Application: WB
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