Human CD25/IL-2R alpha Antibody Summary
Glu22-Cys213
Accession # P01589
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples

Detection of Human CD25/IL-2R alpha by Western Blot. Western blot shows lysates of HDLM-2 human Hodgkin's lymphoma cell line and PC-3 human prostate cancer cell line (negative control). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human CD25/IL-2R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-223-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CD25/IL-2R alpha at approximately 60 kDa (as indicated). GAPDH (Catalog # AF5718) is shown as a loading control.This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Cell Proliferation Induced by IL‑2 and Neutralization by Human CD25/IL‑2 R alpha Antibody. Recombinant Human IL-2 (Catalog # 202-IL) stimulates proliferation in the N1186 human T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-2 (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CD25/IL-2 Ra Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-223-NA). The ND50is typically 0.2-1 µg/mL.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD25/IL-2R alpha
Human CD25, also known as IL-2 receptor alpha (IL-2 R alpha ) and asTac antigen, was initially identified as a 55 kDa membrane glycoprotein that is capable of binding IL‑2. The IL-2 R alpha cDNA encodes a 272 amino acid residue precursor Type I membrane protein with a 21 residue signal peptide, a 219 residue extracellular region, a 19 residue transmembrane region and a 13 residue cytoplasmic domain. IL-2 R alpha lacks structural features characteristic of members of the cytokine receptor superfamily. By itself, IL-2 R alpha binds IL-2 with low affinity. However, when IL-2 R alpha is associated with the IL-2 receptor beta and gamma chains, a high affinity heterotrimeric receptor complex that transduces IL-2 signals is formed. Soluble forms of many cytokine receptors have been reported, and a soluble form of IL-2 R alpha (IL-2 sR alpha ) appears in serum, concomitant with its increased expression on cells. The function of the soluble IL-2 R alpha is unclear. Increased levels of IL-2 sR alpha in biological fluids reportedly correlate with increased T and B cell activation and immune system activation. Increased serum concentration of IL-2 sR alpha has been observed in patients with a variety of inflammatory conditions and in the course of some leukemias and lymphomas.
Product Datasheets
Citations for Human CD25/IL-2R alpha Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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Late expression of granulysin by microbicidal CD4+ T cells requires PI3K- and STAT5-dependent expression of IL-2Rbeta that is defective in HIV-infected patients.
Authors: Zheng CF, Jones GJ, Shi M, Wiseman JC, Marr KJ, Berenger BM, Huston SM, Gill MJ, Krensky AM, Kubes P, Mody CH
J. Immunol., 2008;180(11):7221-9.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Identification of their epitope reveals the structural basis for the mechanism of action of the immunosuppressive antibodies basiliximab and daclizumab.
Authors: Binder M, Vogtle FN, Michelfelder S, Muller F, Illerhaus G, Sundararajan S, Mertelsmann R, Trepel M
Cancer Res., 2007;67(8):3518-23.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Interleukin-2alpha receptor in membrane lipid rafts.
Authors: Li QR, Ma J, Wang H, Li JS
Transplant. Proc., 2005;37(5):2395-7.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Intracellular trafficking of Gag and Env proteins and their interactions modulate pseudotyping of retroviruses.
Authors: Sandrin V, Muriaux D, Darlix JL, Cosset FL
J. Virol., 2004;78(13):7153-64.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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Average Rating: 4.5 (Based on 2 Reviews)
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