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Human CD25/IL-2 R alpha Antibody

  • Specificity
    Detects human CD25/IL-2 R alpha in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL‑2 R gamma or rhIL‑15 R alpha is observed.
  • Source
    Monoclonal Mouse IgG2A Clone # 24212
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD25/IL-2 R alpha
    Glu22-Cys213
    Accession # P01589
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human CD25/IL‑2 R alpha Fc Chimera (Catalog # 1020-RL)
  • Flow Cytometry
    2.5 µg/106 cells
    Human Treg cells (PBMC treated overnight with anti-human CD3 (10 μg/mL; Catalog # MAB100), anti-human CD28 (5 μg/mL; Catalog # MAB342), TGF-beta (10 ng/mL; Catalog # 100-B), and rhIL-2 (20 ng/mL; Catalog # 202-IL).
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD25/IL-2 R alpha
IL-2 R alpha is a type I transmembrane protein that associates with IL-2 R beta and the common gamma chain to form a high affinity heterotrimeric receptor complex for IL-2. A soluble form of IL-2 R alpha has been detected in serum.
  • Long Name:
    Interleukin 2 Receptor alpha
  • Entrez Gene IDs:
    25704 (Human); 16184 (Human); 3559 (Human); 403870 (Canine);
  • Alternate Names:
    CD25; IL-2 R alpha; IL2RA; CD25; CD25 antigen; IDDM10; IL-2 receptor subunit alpha; IL2R; IL-2R subunit alpha; IL-2-RA; IL2-RA; interleukin 2 receptor, alpha; interleukin-2 receptor subunit alpha; p55; TAC antigen; TCGFR
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

Showing Results 1 - 3 of 3
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Species
Applications
Sample Type
  1. Functional characterization of EBV-encoded nuclear antigen 1-specific CD4+ helper and regulatory T cells elicited by in vitro peptide stimulation.
    Authors: Voo KS, Peng G, Guo Z, Fu T, Li Y, Frappier L, Wang RF
    Cancer Res., 2005;65(4):1577-86.
    Species: Human
    Application: Flow Cytometry,
  2. A novel human CD4+ T-cell inducer subset with potent immunostimulatory properties.
    Authors: Ndhlovu LC, Leal FE, Eccles-James IG, Jha AR, Lanteri M, Norris PJ, Barbour JD, Wachter DJ, Andersson J, Tasken K, Torheim EA, Aandahl EM, Kallas EG, Nixon DF
    Eur. J. Immunol., 2010;40(1):134-41.
    Species: Human
    Application: Flow Cytometry,
  3. Human intestinal intraepithelial lymphocytes keep TNF alpha levels low by cell uptake and feedback inhibition of transcription.
    Authors: Ebert EC, Mehta V
    Cell. Immunol., 2006;241(1):7-13.
    Species: Human
    Application: Flow Cytometry,

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