Human CD25/IL-2R alpha Antibody

Catalog # Availability Size / Price Qty
MAB623-SP
MAB623-100
MAB623-500
Detection of Recombinant Human and Rat CD25/IL-2 R alpha by Western Blot.
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Product Details
Citations (3)
FAQs
Supplemental Products
Reviews (2)

Human CD25/IL-2R alpha Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD25/IL-2 R alpha in ELISAs and Western blots. In Western blots, detects recombinant rat CD25/IL-2 R alpha but no cross-reactivity with recombinant mouse CD25/IL-2 R alpha. In sandwich immunoassays, this antibody does not cross-react or interfere with recombinant human (rh) IL-1 RI, rhIL-1 RII, rhIL-2 R beta, rhIL-3 R alpha, rhIL-4 R, rhIL-5 R alpha, rhIL-5 R beta, rhIL-6 R, or rhgp130.
Source
Monoclonal Mouse IgG1 Clone # 24204
Purification
Protein A or G purified from ascites
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD25/IL-2 R alpha
Glu22-Cys213
Accession # P01589
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
8-25 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below

Human CD25/IL-2 R alpha Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human CD25/IL-2R alpha Biotinylated Antibody (Catalog # BAF223)

Standard: Recombinant Human CD25/IL-2R alpha Protein (Catalog # 223-2A)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Recombinant Human and Rat CD25/IL-2 R alpha antibody by Western Blot. View Larger

Detection of Recombinant Human and Rat CD25/IL-2 R alpha by Western Blot. Western blot shows 25 ng of Recombinant Human CD25/IL-2 R alpha (Catalog # 223-2A), Recombinant Mouse CD25/IL-2 R alpha (Catalog # 2438-RM) and Recombinant Rat CD25/IL-2 R alpha Fc Chimera (Catalog # 5156-RM). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human CD25/IL-2 R alpha Monoclonal Antibody (Catalog # MAB623) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for CD25/IL-2 R alpha at approximately 30-250 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 3.

Immunocytochemistry CD25/IL-2 Ra antibody in Human PBMCs by Immunocytochemistry (ICC). View Larger

CD25/IL‑2 R alpha in Human PBMCs. CD25/IL-2 Ra was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with PHA using Mouse Anti-Human CD25/IL-2 Ra Monoclonal Antibody (Catalog # MAB623) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). Upper panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunohistochemistry CD25/IL-2 Ra antibody in Human Lymph Node by Immunohistochemistry (IHC-Fr). View Larger

CD25/IL‑2 R alpha in Human Lymph Node. CD25/IL‑2 R alpha was detected in immersion fixed frozen sections of human lymph node using 15 µg/mL Mouse Anti-Human CD25/IL‑2 R alpha Monoclonal Antibody (Catalog # MAB623) overnight at 4 °C. Tissue was stained (red) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD25/IL-2R alpha

IL-2 R alpha is a type I transmembrane protein that associates with IL-2 R beta and the common gamma chain to form a high affinity heterotrimeric receptor complex for IL-2. A soluble form of IL-2 R alpha has been detected in serum.

Long Name
Interleukin 2 Receptor alpha
Entrez Gene IDs
3559 (Human); 16184 (Mouse); 25704 (Rat); 403870 (Canine); 102123605 (Cynomolgus Monkey)
Alternate Names
CD25 antigen; CD25; IDDM10; IL-2 R alpha; IL-2 receptor subunit alpha; IL2R alpha; IL-2R subunit alpha; IL2R; IL2RA; IL-2Ra; IL-2-RA; IL2-RA; interleukin 2 receptor, alpha; interleukin-2 receptor subunit alpha; p55; TAC antigen; TCGFR

Product Datasheets

Citations for Human CD25/IL-2R alpha Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
    Authors: Hu H, Kwun J, Aizenstein BD, Knechtle SJ
    Transplantation, 2009;87(12):1814-20.
    Species: Human
    Sample Types: Urine
    Applications: Antibody Array Development
  2. Imbalance in the peritoneal levels of interleukin 1 and its decoy inhibitory receptor type II in endometriosis women with infertility and pelvic pain.
    Authors: Akoum A, Al-Akoum M, Lemay A, Maheux R, Leboeuf M
    Fertil. Steril., 2007;89(6):1618-24.
    Species: Human
    Sample Types: Peritoneal Fluid
    Applications: ELISA Development
  3. Induction of lysosomal and plasma membrane-bound sialidases in human T-cells via T-cell receptor.
    Authors: Wang P, Zhang J, Bian H, Wu P, Kuvelkar R, Kung TT, Crawley Y, Egan RW, Billah MM
    Biochem. J., 2004;380(0):425-33.
    Species: Human
    Sample Types:
    Applications: Western Blot

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Isotype Controls

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Reviews for Human CD25/IL-2R alpha Antibody

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Human CD25/IL-2R alpha Antibody
By Anonymous on 09/08/2021
Application: IHC Sample Tested: Tonsil tissue Species: Human

Human CD25/IL-2R alpha Antibody
By Anonymous on 07/23/2019
Application: ELISA Sample Tested: EDTA Plasma Species: Human