Human CD38 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human CD38.
  • Source
    Monoclonal Mouse IgG2A Clone # 240742
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    NS0 mouse myeloma cell line transfected with human CD38
    Met1-Ile300
    Accession # P28907
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    2.5 µg/106 cells
    Human whole blood lymphocytes
  • Immunoprecipitation
    25 µg/mL
    Conditioned cell culture medium spiked with Recombinant Human CD38 (Catalog # 2404‑AC), see our available Western blot detection antibodies
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
CD38 in Human PBMCs. CD38 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using 10 µg/mL Mouse Anti-Human CD38 Monoclonal Antibody (Catalog # MAB2404) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD38

CD38, also known as ADP-ribosyl cyclase, is a Type II integral membrane protein. The enzyme is able to transform NAD(P)+ into three different products with calcium mobilizing ability, cyclic ADP-ribose, NAADP+, and ADP-ribose (1). CD38 is expressed in B and T lymphocytes, osteoclasts, and in cardiac, pancreatic, liver and kidney cells (2, 3). Through its production of cyclic ADP-ribose, CD38 modulates calcium-mediated signal transduction in many types of cells, including neutrophils and pancreatic beta  cells (4, 5).

  • References:
    1. Schuber, F. and F.E. Lund (2004) Curr. Mol. Med. 4:249.
    2. Jackson, D.G. and J.I. Bell (1990) J. Immunol. 144:2811.
    3. Sun, L. et al. (1999) J. Cell Biol. 146:1161.
    4. Partida-Sanchez, S. et al. (2001) Nature Med. 7:1209.
    5. Kato, I. et al. (1995) J. Biol. Chem. 270:30045.
  • Long Name:
    Cluster of Differentiation 38
  • Entrez Gene IDs:
    952 (Human); 12494 (Mouse); 25668 (Rat); 102126394 (Cynomolgus Monkey)
  • Alternate Names:
    ADP-ribosyl cyclase 1; ADP-ribosyl Cyclase; ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase; cADPr hydrolase 1; CD38 antigen (p45); CD38 antigen; CD38 molecule; CD38; Cyclic ADP-ribose hydrolase 1; Cyclic ADP-ribose Hydrolase; EC 3.2.2.5; NAD(+) nucleosidase; T10
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