Human CD44s Pan Specific Antibody

Catalog # Availability Size / Price Qty
MAB7045
MAB7045-SP
Detection of Human CD44 by Western Blot.
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Product Details
Citations (4)
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Reviews (2)

Human CD44s Pan Specific Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD44 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant CD44 from mouse, rat, or pig is observed.
Source
Monoclonal Mouse IgG2A Clone # 691534
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CD44s

Gln21-Pro220
Accession # P16070
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

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Applications

Recommended Concentration
Sample
Western Blot
0.2 µg/mL
See below
Flow Cytometry
2.5 µg/106 cells
See below
Immunohistochemistry
8-25 µg/mL
See below
CyTOF-reported
This clone has been commercially reported for use in CyTOF®. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human CD44 antibody by Western Blot. View Larger

Detection of Human CD44 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HUVEC human umbilical vein endothelial cells, and PC-3 human prostate cancer cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human CD44s Pan Specific Monoclonal Antibody (Catalog # MAB7045) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for CD44 at approximately 80 to 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection of CD44 antibody in Human Blood Lymphocytes antibody by Flow Cytometry. View Larger

Detection of CD44 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD44s Pan Specific Monoclonal Antibody (Catalog # MAB7045, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophy-cocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Immunocytochemistry CD44 antibody in Human PBMCs by Immunocytochemistry (ICC). View Larger

CD44 in Human PBMCs. CD44 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human CD44s Pan Specific Monoclonal Antibody (Catalog # MAB7045) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunohistochemistry CD44 antibody in Human Tonsil by Immunohistochemistry (IHC-P). View Larger

CD44 in Human Tonsil. CD44 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human CD44s Pan Specific Monoclonal Antibody (Catalog # MAB7045) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD44

CD44 is a ubiquitously expressed protein that is the major receptor for hyaluronan and exerts control over cell growth and migration (1‑3). Human CD44 has a 20 amino acid (aa) signal sequence, an extracellular domain (ECD) with a 100 aa hyaluronan-binding disulfide-stabilized link region and a 325‑530 aa stem region, a 21 aa transmembrane domain, and a 72 aa cytoplasmic domain. Within the stem, ten variably spliced exons (v1‑10, exons 6‑15) produce multiple protein isoforms (1‑3). The standard or hematopoietic form, CD44s or CD44H, does not include the variable segments (1‑3). Cancer aggressiveness and T cell activation have been correlated with expression of specific isoforms (1, 3). With variable N- and O-glycosylation and splicing within the stalk, CD44 can range from 80 to 200 kDa (1). Within the N‑terminal invariant portion of the ECD (aa 21‑220), human CD44 shares 76%, 76%, 86%, 83% and 79% identity with corresponding mouse, rat, equine, canine and bovine CD44, respectively. The many reported functions of CD44 fall within three categories (1). First, CD44 binds hyaluronan and other ligands within the extracellular matrix and can function as a “platform” for growth factors and metalloproteinases. Second, CD44 can function as a co‑receptor that modifies activity of receptors including MET and the ERBB family of tyrosine kinases. Third, the CD44 intracellular domain links the plasma membrane to the actin cytoskeleton via the ERM proteins, ezrin, radixin and moesin. CD44 can be synthesized in a soluble form (4) or may be cleaved at multiple sites by either membrane-type matrix metalloproteinases, or ADAM proteases to produce soluble ectodomains (5, 6). The cellular portion may then undergo gamma secretase-dependent intramembrane cleavage to form an A beta -like transmembrane portion and a cytoplasmic signaling portion that affects gene expression (7, 8). These cleavage events are thought to promote metastasis by enhancing tumor cell motility and growth (1, 5).

References
  1. Ponta, H. et al. (2003) Nat. Rev. Mol. Cell Biol. 4:33.
  2. Screaton, G.R. et al. (1992) Proc. Natl. Acad. Sci. USA 89:12160.
  3. Lynch, K.W. (2004) Nat. Rev. Immunol. 4:931.
  4. Yu, Q. and B.P. Toole (1996) J. Biol. Chem. 271:20603.
  5. Nagano, O. and H. Saya (2004) Cancer Sci. 95:930.
  6. Nakamura, H. et al. (2004) Cancer Res. 64:876.
  7. Murakami, D. et al. (2003) Oncogene 22:1511.
  8. Lammich, S. et al. (2002) J. Biol. Chem. 277:44754.
Entrez Gene IDs
960 (Human); 12505 (Mouse); 25406 (Rat); 100126860 (Porcine)
Alternate Names
CD44 antigen; CD44 molecule (Indian blood group); CD44; CD44R; CDw44; cell surface glycoprotein CD44; chondroitin sulfate proteoglycan 8; CSPG8; ECMR-III; epican; Extracellular matrix receptor III; GP90 lymphocyte homing/adhesion receptor; HCAM; HCELL; hematopoietic cell E- and L-selectin ligand; Heparan sulfate proteoglycan; Hermes antigen; homing function and Indian blood group system; HUTCH-I; Hyaluronate receptor; IN; LHR; MC56; MDU2; MDU2CD44 antigen (homing function and Indian blood group system); MDU3; MDU3CDW44; MIC4; MIC4MGC10468; MUTCH-I; Pgp1; PGP-1; PGP-I; Phagocytic glycoprotein 1; Phagocytic glycoprotein I

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Citations for Human CD44s Pan Specific Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Inhibition of resistant triple-negative breast cancer cells with low-dose 6-mercaptopurine and 5-azacitidine
    Authors: B Singh, VN Sarli, A Lucci
    Oncotarget, 2021;12(7):626-637.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Mammospheres of letrozole-resistant breast cancer cells enhance breast cancer aggressiveness
    Authors: JR Patel, KM Gallegos, RR Walker, AM Davidson, I Davenport, SL Tilghman
    Oncology Letters, 2021;22(2):620.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Clinical impact of different exosomes' protein expression in pancreatic ductal carcinoma patients treated with standard first line palliative chemotherapy
    Authors: R Giampieri, F Piva, G Occhipinti, A Bittoni, A Righetti, S Pagliarett, A Murrone, F Bianchi, C Amantini, M Giulietti, G Ricci, G Principato, G Santoni, R Berardi, S Cascinu
    PLoS ONE, 2019;14(5):e0215990.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Detection
  4. SALL4 promotes gastric cancer progression through activating CD44 expression
    Oncogenesis, 2016;5(11):e268.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P

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Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

Reviews for Human CD44s Pan Specific Antibody

Average Rating: 4.5 (Based on 2 Reviews)

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Human CD44s Pan Specific Antibody
By Anonymous on 06/12/2019
Application: ELISA Sample Tested: Serum and Plasma Species: Human

Human CD44s Pan Specific Antibody
By Anonymous on 01/30/2019
Application: ELISA Sample Tested: Serum and Plasma Species: Human

MAB7045 was used as the capture antibody along with BBA10 as the detection antibody. Recombinant human CD44 Fc Chimera (3660-CD) was used as the calibrator material. Human serum and plasma samples were diluted 1:5 and all were quantifiable. Parallelism looked good.