Human CD44v3 Antibody

(5 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human CD44v3 in Western blots. The specificity of monoclonal antibody clone 3G5 was determined by FACS analysis on a panel of CD44 transfected COS cells, and was deduced to be specific for CD44 protein isoforms containing human variant exon 3 (9).
  • Source
    Monoclonal Mouse IgG2B Clone # 3G5
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Recombinant human CD44v3-10
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human CD44 Fc Chimera (Catalog # 3660-CD)
  • Flow Cytometry
    2.5 µg/106 cells
    MDA‑MB‑231 human breast cancer cell line
  • Immunoprecipitation
    Fox, S.B. et al. (1994) Cancer Res. 54:4539.
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
CD44 in Human PBMCs. CD44 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using 8 µg/mL Mouse Anti-Human CD44 v3 Monoclonal Antibody (Catalog # BBA11) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD44

CD44 is a ubiquitously expressed protein that is the major receptor for hyaluronan and exerts control over cell growth and migration (1‑3). Human CD44 has a 20 amino acid (aa) signal sequence, an extracellular domain (ECD) with a 100 aa hyaluronan-binding disulfide-stabilized link region and a 325‑530 aa stem region, a 21 aa transmembrane domain, and a 72 aa cytoplasmic domain. Within the stem, ten variably spliced exons (v1‑10, exons 6‑15) produce multiple protein isoforms (1‑3). The standard or hematopoietic form, CD44H, does not include the variable segments (1‑3). Cancer aggressiveness and T cell activation have been correlated with expression of specific isoforms (1, 3). With variable N‑ and O-glycosylation and splicing within the stalk, CD44 can range from 80 to 200 kDa (1). Within the N‑terminal invariant portion of the ECD (aa 21‑220), human CD44 shares 76%, 76%, 86%, 83% and 79% aa sequence identity with corresponding mouse, rat, equine, canine and bovine CD44, respectively. The many reported functions of CD44 fall within three categories (1). First, CD44 binds hyaluronan and other ligands within the extracellular matrix and can function as a “platform” for growth factors and metalloproteinases. Second, CD44 can function as a co-receptor that modifies activity of receptors including MET and the ERBB family of tyrosine kinases. Third, the CD44 intracellular domain links the plasma membrane to the actin cytoskeleton via the ERM proteins, ezrin, radixin and moesin. CD44 can be synthesized in a soluble form (4) or may be cleaved at multiple sites by either membrane-type matrix metalloproteinases, or ADAM proteases to produce soluble ectodomains (5, 6). The cellular portion may then undergo gamma secretase-dependent intramembrane cleavage to form an A beta -like transmembrane portion and a cytoplasmic signaling portion that affects gene expression (7, 8). These cleavage events are thought to promote metastasis by enhancing tumor cell motility and growth (1, 5).

  • References:
    1. Ponta, H. et al. (2003) Nat. Rev. Mol. Cell Biol. 4:33.
    2. Screaton, G.R. et al. (1992) Proc. Natl. Acad. Sci. USA 89:12160.
    3. Lynch, K.W. (2004) Nat. Rev. Immunol. 4:931.
    4. Yu, Q. and B.P. Toole (1996) J. Biol. Chem. 271:20603.
    5. Nagano, O. and H. Saya (2004) Cancer Sci. 95:930.
    6. Nakamura, H. et al. (2004) Cancer Res. 64:876.
    7. Murakami, D. et al. (2003) Oncogene 22:1511.
    8. Lammich, S. et al. (2002) J. Biol. Chem. 277:44754.
    9. Fox, S.B. et al. (1994) Cancer Res. 54:4539.
  • Entrez Gene IDs:
    960 (Human); 12505 (Mouse); 25406 (Rat); 100126860 (Porcine)
  • Alternate Names:
    CD44 antigen; CD44 molecule (Indian blood group); CD44; CD44R; CDw44; cell surface glycoprotein CD44; chondroitin sulfate proteoglycan 8; CSPG8; ECMR-III; epican; Extracellular matrix receptor III; GP90 lymphocyte homing/adhesion receptor; HCAM; HCELL; hematopoietic cell E- and L-selectin ligand; Heparan sulfate proteoglycan; Hermes antigen; homing function and Indian blood group system; HUTCH-I; Hyaluronate receptor; IN; LHR; MC56; MDU2; MDU2CD44 antigen (homing function and Indian blood group system); MDU3; MDU3CDW44; MIC4; MIC4MGC10468; MUTCH-I; Pgp1; PGP-1; PGP-I; Phagocytic glycoprotein 1; Phagocytic glycoprotein I
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Species
Applications
Sample Type
  1. MIF allele-dependent regulation of the MIF coreceptor CD44 and role in rheumatoid arthritis
    Proc. Natl. Acad. Sci. U.S.A., 2016;113(49):E7917-E7926.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  2. Overexpression of progelatinase B/proMMP-9 affects migration regulatory pathways and impairs chronic lymphocytic leukemia cell homing to bone marrow and spleen.
    Authors: Bailon E, Ugarte-Berzal E, Amigo-Jimenez I, Van den Steen P, Opdenakker G, Garcia-Marco J, Garcia-Pardo A
    J Leukoc Biol, 2014;96(2):185-99.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. Epstein-Barr virus infection of polarized epithelial cells via the basolateral surface by memory B cell-mediated transfer infection.
    Authors: Shannon-Lowe C, Rowe M
    PLoS Pathog., 2011;7(5):e1001338.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  4. Expression of the CD44v2-10 isoform confers a metastatic phenotype: importance of the heparan sulfate attachment site CD44v3.
    Authors: Barbour AP, Reeder JA, Walsh MD, Fawcett J, Antalis TM, Gotley DC
    Cancer Res., 2003;63(4):887-92.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  5. CD44 is exposed to the extracellular matrix at invasive sites in basal cell carcinomas.
    Authors: Dingemans KP, Ramkema MD, Pals ST
    Lab. Invest., 2002;82(3):313-22.
    Species: Human
    Sample Type: Whole Cells
    Application: Electron Microscopy
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