Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Flow Cytometry, Immunocytochemistry, Immunoprecipitation, CyTOF-ready

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Flow Cytometry, Immunocytochemistry, Immunogold Electron Microscopy

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 3G5
View all CD44 Primary Antibodies »

Product Specifications

Immunogen

Recombinant human CD44v3-10

Specificity

Detects human CD44v3 in Western blots. The specificity of monoclonal antibody clone 3G5 was determined by FACS analysis on a panel of CD44 transfected COS cells, and was deduced to be specific for CD44 protein isoforms containing human variant exon 3 (9).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human CD44v3 Antibody

CD44 antibody in Human PBMCs by Immunocytochemistry (ICC).

CD44 in Human PBMCs.

CD44 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using 8 µg/mL Mouse Anti-Human CD44 v3 Monoclonal Antibody (Catalog # BBA11) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Detection of CD44v3 in Human MDA-MB-231 by Flow Cytometry.

MDA-MB-231 human breast adenocarcinoma cell line was stained with Mouse Anti-Human CD44s Pan Specific Monoclonal Antibody (Catalog # MAB7045, filled histogram) or isotype control antibody (MAB003, open histogram), followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (F0101B).

Applications for Human CD44v3 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: MDA‑MB‑231 human breast cancer cell line

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells subjected to microwave antigen retrieval

Immunoprecipitation

Fox, S.B. et al. (1994) Cancer Res. 54:4539.

Western Blot

1 µg/mL
Sample: Recombinant Human CD44 Fc Chimera (Catalog # 3660-CD)

Reviewed Applications

Read 2 reviews rated 4.5 using BBA11 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD44

CD44 is a ubiquitously expressed protein that is the major receptor for hyaluronan and exerts control over cell growth and migration (1‑3). Human CD44 has a 20 amino acid (aa) signal sequence, an extracellular domain (ECD) with a 100 aa hyaluronan-binding disulfide-stabilized link region and a 325‑530 aa stem region, a 21 aa transmembrane domain, and a 72 aa cytoplasmic domain. Within the stem, ten variably spliced exons (v1‑10, exons 6‑15) produce multiple protein isoforms (1‑3). The standard or hematopoietic form, CD44H, does not include the variable segments (1‑3). Cancer aggressiveness and T cell activation have been correlated with expression of specific isoforms (1, 3). With variable N‑ and O-glycosylation and splicing within the stalk, CD44 can range from 80 to 200 kDa (1). Within the N‑terminal invariant portion of the ECD (aa 21‑220), human CD44 shares 76%, 76%, 86%, 83% and 79% aa sequence identity with corresponding mouse, rat, equine, canine and bovine CD44, respectively. The many reported functions of CD44 fall within three categories (1). First, CD44 binds hyaluronan and other ligands within the extracellular matrix and can function as a “platform” for growth factors and metalloproteinases. Second, CD44 can function as a co-receptor that modifies activity of receptors including MET and the ERBB family of tyrosine kinases. Third, the CD44 intracellular domain links the plasma membrane to the actin cytoskeleton via the ERM proteins, ezrin, radixin and moesin. CD44 can be synthesized in a soluble form (4) or may be cleaved at multiple sites by either membrane-type matrix metalloproteinases, or ADAM proteases to produce soluble ectodomains (5, 6). The cellular portion may then undergo gamma secretase-dependent intramembrane cleavage to form an A beta -like transmembrane portion and a cytoplasmic signaling portion that affects gene expression (7, 8). These cleavage events are thought to promote metastasis by enhancing tumor cell motility and growth (1, 5).

References

  1. Ponta, H. et al. (2003) Nat. Rev. Mol. Cell Biol. 4:33.
  2. Screaton, G.R. et al. (1992) Proc. Natl. Acad. Sci. USA 89:12160.
  3. Lynch, K.W. (2004) Nat. Rev. Immunol. 4:931.
  4. Yu, Q. and B.P. Toole (1996) J. Biol. Chem. 271:20603.
  5. Nagano, O. and H. Saya (2004) Cancer Sci. 95:930.
  6. Nakamura, H. et al. (2004) Cancer Res. 64:876.
  7. Murakami, D. et al. (2003) Oncogene 22:1511.
  8. Lammich, S. et al. (2002) J. Biol. Chem. 277:44754.
  9. Fox, S.B. et al. (1994) Cancer Res. 54:4539.

Alternate Names

CD44, ECMR-III, HCAM, HCELL, LHR, MDU2, MDU3, MIC4, MUTCH-I, Pgp1

Entrez Gene IDs

960 (Human); 12505 (Mouse); 25406 (Rat); 100126860 (Porcine)

Gene Symbol

CD44

Additional CD44 Products

Product Documents for Human CD44v3 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CD44v3 Antibody

For research use only

Citations for Human CD44v3 Antibody

Customer Reviews for Human CD44v3 Antibody (2)

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  • Human CD44v3 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 11/08/2018
  • Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: See PMID: 24620030
    Species: Human
    Verified Customer | Posted 01/16/2015

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