Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, Immunocytochemistry, Simple Western

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 1093310
View all CFL1 Primary Antibodies »

Product Specifications

Immunogen

Synthetic Peptide
Accession # P23528

Specificity

Detects a synthetic peptide specific for human CFL1 around aa 30 in Direct ELISA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human CFL1 Antibody

Detection of Human, Mouse and Rat CFL1 by Western Blot.

Western Blot shows lysates of Jurkat human acute T cell leukemia cell line, MCF‑7 human breast cancer cell line, NIH‑3T3 mouse embryonic fibroblast cell line and C6 rat glioma cell line. PVDF membrane was probed with 0.5 µg/ml of Mouse Anti-Human CFL1 Monoclonal Antibody (Catalog # MAB11669) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CFL1 at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Detection of CFL1 in Liver

CFL1 was detected in immersion fixed paraffin-embedded sections of liver using Mouse Anti-Human CFL1 Monoclonal Antibody (Catalog # MAB11669) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the nucleus and cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of CFL1 in MCF‑7 Human Cell Line.

CFL1 was detected in immersion fixed MCF‑7 human breast cancer cell line using Mouse Anti-Human CFL1 Monoclonal Antibody (Catalog # MAB11669) at 8 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the nucleus and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Human CFL1 by Simple WesternTM.

Simple Western shows lysates of Exosome Standards (LNCaP) (NBP3-11687), Exosome Standards (PC‑3) (NBP2-49856) and MCF‑7 human breast cancer cell line, loaded at 0.5 mg/ml. A specific band was detected for CFL1 at approximately 24 kDa (as indicated) using 10-100 µg/mL of Mouse Anti-Human CFL1 Monoclonal Antibody (Catalog # MAB11669). This experiment was conducted under reducing conditions and using the 2-40 kDa separation system.

Applications for Human CFL1 Antibody

Application
Recommended Usage

Immunocytochemistry

3-25 µg/mL
Sample: Immersion fixed MCF-7 human breast cancer cell line

Immunohistochemistry

3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of liver

Simple Western

10-100 µg/mL
Sample: Exosome Standards (LNCaP) (Catalog # NBP3-11687), Exosome Standards (PC-3) (Catalog # NBP2-49856) and MCF-7 human breast cancer cell line

Western Blot

0.5 µg/mL
Sample: Jurkat human acute T cell leukemia cell line, MCF-7 human breast cancer cell line, NIH-3T3 mouse embryonic fibroblast cell line and C6 rat glioma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CFL1

Cofilin-1 (CFL1) is an 18.5 kDa protein that is a member of the actin deploy family (ADF). It is involved in the biological activities of various malignancies, including infiltration, metastasis, and chemotherapy resistance. Overexpression of CFL is associated with aggressive features and poor prognosis in malignancies. CFL1 also plays a crucial role in B cell, T cell infiltration, cytokine and NF-kappa B signaling pathways and can serve as a useful biomarker for diagnosis of inflammatory diseases.  

References

  1. Yin X, Li X, Jiang H, Lin X, Ma Z, Chen X, Teng Q, Zhang J, Jin J. CFL1 is Implicated in Chronic Myeloid Leukemia Response during Imatinib Therapy. J Cancer. 2024 Mar 4;15(8):2424-2430. doi: 10.7150/jca.92202. PMID: 38495482; PMCID: PMC10937266. 
  2. Xing J, Wang Y, Peng A, Li J, Niu X, Zhang K. The role of actin cytoskeleton CFL1 and ADF/cofilin superfamily in inflammatory response. Front Mol Biosci. 2024 Jul 24;11:1408287. doi: 10.3389/fmolb.2024.1408287. PMID: 39114368; PMCID: PMC11303188.

Long Name

cofilin 1

Alternate Names

CFL, cofilin, cofilin-1, HEL-S-15

Entrez Gene IDs

1072 (Human); 12631 (Mouse); 29271 (Rat)

Gene Symbol

CFL1

UniProt

P23528 (Human)

Additional CFL1 Products

Product Documents for Human CFL1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human CFL1 Antibody

For research use only

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Protocols

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