Human CFL1 Antibody

Detection of Human, Mouse and Rat CFL1 by Western Blot. Western Blot shows lysates of Jurkat human acute T cell leukemia cell line, MCF‑7 human breast cancer cell line, NIH‑3T3 mouse embryonic fibroblast cell line and C6 rat glioma cell line. PVDF membrane was probed with 0.5 µg/ml of Mouse Anti-Human CFL1 Monoclonal Antibody (Catalog # MAB11669) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CFL1 at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Detection of CFL1 in Liver CFL1 was detected in immersion fixed paraffin-embedded sections of liver using Mouse Anti-Human CFL1 Monoclonal Antibody (Catalog # MAB11669) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the nucleus and cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of CFL1 in MCF‑7 Human Cell Line. CFL1 was detected in immersion fixed MCF‑7 human breast cancer cell line using Mouse Anti-Human CFL1 Monoclonal Antibody (Catalog # MAB11669) at 8 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the nucleus and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Human CFL1 by Simple Western^TM. Simple Western shows lysates of Exosome Standards (LNCaP) (NBP3-11687), Exosome Standards (PC‑3) (NBP2-49856) and MCF‑7 human breast cancer cell line, loaded at 0.5 mg/ml. A specific band was detected for CFL1 at approximately 24 kDa (as indicated) using 10-100 µg/mL of Mouse Anti-Human CFL1 Monoclonal Antibody (Catalog # MAB11669). This experiment was conducted under reducing conditions and using the 2-40 kDa separation system.