Human Cortactin Antibody (771716)

R&D Systems | Catalog # MAB6096

R&D Systems

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Knockout Validated, Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 771716
View all Cortactin Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Cortactin
Met1-Gly85
Accession # Q14247

Specificity

Detects human Cortactin in ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Cortactin Antibody (771716)

Detection of Human Cortactin antibody by Western Blot.

Detection of Human Cortactin by Western Blot.

Western blot shows lysates of A549 human lung carcinoma cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human Cortactin Monoclonal Antibody (Catalog # MAB6096) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for Cortactin at approximately 80-85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Cortactin antibody in Human Prostate by Immunohistochemistry (IHC-P).

Cortactin in Human Prostate.

Cortactin was detected in immersion fixed paraffin-embedded sections of human prostate using Mouse Anti-Human Cortactin Monoclonal Antibody (Catalog # MAB6096) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the plasma membranes of glandular epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Western Blot Shows Human Cortactin Antibody Specificity by Using Knockout Cell Line.

Western Blot Shows Human Cortactin Specificity by Using Knockout Cell Line.

Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and TACE/ADAM17 knockout HeLa cell line (KO). PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human Cortactin Monoclonal Antibody (Catalog # MAB6096) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Cortactin at approximately 80 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Cortactin Antibody (771716)

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human prostate

Knockout Validated

Cortactin is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in Cortactin knockout HeLa cell line.

Western Blot

0.1 µg/mL
Sample: A549 human lung carcinoma cell line and K562 human chronic myelogenous leukemia cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Cortactin

Cortactin (also EMS1 and Amplaxin) is an 80‑85 kDa multidomain scaffold-associated protein. It is an actin-binding cytoplasmic protein found at intercellular junctions and near lamellipodia. It is also found in tumor invadopodia, a somewhat analogous structure to lamellipodia. Here, Cortactin appears to coordinate actin assembly with MMP secretion, thus facilitating tumor invasiveness. Human Cortactin is 550 amino acids (aa) in length and contains six-plus Cortactin regions (aa 80‑324) that regulate actin polymerization, and one SH3 domain (aa 496‑548) that binds to N-WASP. Cortactin is highly phosphorylated on Tyr and Ser/Thr, and undergoes acetylation.

Alternate Names

Amplaxin, CTTN, EMS1

Entrez Gene IDs

2017 (Human); 13043 (Mouse); 60465 (Rat)

Gene Symbol

CTTN

UniProt

Q14247 (Human)

Additional Cortactin Products

Product Documents for Human Cortactin Antibody (771716)

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Product Specific Notices for Human Cortactin Antibody (771716)

For research use only

Citations for Human Cortactin Antibody (771716)

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Protocols

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