Human COX-2 Antibody Summary
Ala18-Ser112 and Gln386-Leu604
Accession # P35354
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human COX‑2 by Western Blot. Western blot shows lysates of human peripheral blood mononuclear cells (PBMC) untreated (-) or treated (+) with LPS. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human COX-2 Monoclonal Antibody (Catalog # MAB4198), followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for COX-2 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
COX‑2 in A549 Human Cell Line. COX-2 was detected in immersion fixed A549 human lung carcinoma cell line using Mouse Anti-Human COX-2 Monoclonal Antibody (Catalog # MAB4198) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
COX‑2 in Human Breast Cancer Tissue. COX-2 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human COX-2 Monoclonal Antibody (Catalog # MAB4198) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Cyclooxygenase-2 (COX-2) also known as prostaglandin G/H synthase 2 (PGHS2) is a 70 kDa microsomal enzyme that belongs to the prostaglandin G/H synthase family. It is inducibly-expressed by a number of cell types, including fibroblasts, vascular smooth muscle cells, endothelium, and monocytes. Functionally, COX-2 is a homodimer that catalyzes two steps in the conversion of arachadonic acid to prostaglandin H2. Mature human COX-2 is 587 amino acids (aa) in length and contains one EGF-like domain (aa 18‑55), a potential membrane interacting region (aa 277‑292) and a globular catalytic domain (aa 293‑604). At least one splice form exists that shows an 11 aa substitution for the C-terminal 451 amino acids. Over the amino acid range of the immunogen, human COX-2 shows 83% aa identity to mouse COX-2.
Citations for Human COX-2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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Evaluation of an Aqueous Extract from Horseradish Root (Armoracia rusticana Radix) against Lipopolysaccharide-Induced Cellular Inflammation Reaction
Authors: C Herz, HT Tran, MR Márton, R Maul, S Baldermann, M Schreiner, E Lamy
Evid Based Complement Alternat Med, 2017;2017(0):1950692.
Sample Types: Whole Cells
Applications: Western Blot
Adipose-derived mesenchymal stem cells modulate CD14(++)CD16(+) expression on monocytes from sepsis patients in vitro via prostaglandin E2
Authors: G Qiu, G Zheng, M Ge, L Huang, H Tong, P Chen, D Lai, Y Hu, B Cheng, Q Shu, J Xu
Stem Cell Res Ther, 2017;8(1):97.
Sample Types: Cell Lysates
Applications: Western Blot
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