Human COX-2 Antibody

Catalog # Availability Size / Price Qty
MAB4198
MAB4198-SP
Human COX-2 Antibody in Data
3 Images
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews (1)

Human COX-2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human COX-2 in Western blots.
Source
Monoclonal Mouse IgG2B Clone # 495222
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human COX-2
Ala18-Ser112 and Gln386-Leu604
Accession # P35354
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
8-25 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human COX‑2 by Western Blot. View Larger

Detection of Human COX‑2 by Western Blot. Western blot shows lysates of human peripheral blood mononuclear cells (PBMC) untreated (-) or treated (+) with LPS. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human COX-2 Monoclonal Antibody (Catalog # MAB4198), followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for COX-2 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Immunocytochemistry COX‑2 in A549 Human Cell Line. View Larger

COX‑2 in A549 Human Cell Line. COX-2 was detected in immersion fixed A549 human lung carcinoma cell line using Mouse Anti-Human COX-2 Monoclonal Antibody (Catalog # MAB4198) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry COX‑2 in Human Breast Cancer Tissue. View Larger

COX‑2 in Human Breast Cancer Tissue. COX-2 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human COX-2 Monoclonal Antibody (Catalog # MAB4198) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: COX-2

Cyclooxygenase-2 (COX-2) also known as prostaglandin G/H synthase 2 (PGHS2) is a 70 kDa microsomal enzyme that belongs to the prostaglandin G/H synthase family. It is inducibly-expressed by a number of cell types, including fibroblasts, vascular smooth muscle cells, endothelium, and monocytes. Functionally, COX-2 is a homodimer that catalyzes two steps in the conversion of arachadonic acid to prostaglandin H2. Mature human COX-2 is 587 amino acids (aa) in length and contains one EGF-like domain (aa 18‑55), a potential membrane interacting region (aa 277‑292) and a globular catalytic domain (aa 293‑604). At least one splice form exists that shows an 11 aa substitution for the C-terminal 451 amino acids. Over the amino acid range of the immunogen, human COX-2 shows 83% aa identity to mouse COX-2.

Long Name
Cyclooxygenase 2
Entrez Gene IDs
5743 (Human); 19225 (Mouse); 29527 (Rat)
Alternate Names
COX2; COX-2; COX2cyclooxygenase 2b; cyclooxygenase-2; EC 1.14.99; EC 1.14.99.1; GRIPGHS; hCox-2; PGG/HS; PGH synthase 2; PGHS-2; PHS II; PHS-2; PHS-II; prostaglandin G/H synthase 2; prostaglandin G/H synthase and cyclooxygenase; Prostaglandin H2 synthase 2; prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase andcyclooxygenase); Prostaglandin-endoperoxide synthase 2; PTGS2

Product Datasheets

Citations for Human COX-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Evaluation of an Aqueous Extract from Horseradish Root (Armoracia rusticana Radix) against Lipopolysaccharide-Induced Cellular Inflammation Reaction
    Authors: C Herz, HT Tran, MR Márton, R Maul, S Baldermann, M Schreiner, E Lamy
    Evid Based Complement Alternat Med, 2017;2017(0):1950692.
    Species: Human
    Sample Types: Whole Cells
    Applications: Western Blot
  2. Adipose-derived mesenchymal stem cells modulate CD14(++)CD16(+) expression on monocytes from sepsis patients in vitro via prostaglandin E2
    Authors: G Qiu, G Zheng, M Ge, L Huang, H Tong, P Chen, D Lai, Y Hu, B Cheng, Q Shu, J Xu
    Stem Cell Res Ther, 2017;8(1):97.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human COX-2 Antibody

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Human COX-2 Antibody
By Anonymous on 11/23/2020
Application: ICC/IF Sample Tested: Melanoma tissue Species: Human