Cytokeratin 14 in NHEK Human Normal Epidermal Keratinocytes.
Cytokeratin 14 was detected in immersion fixed NHEK human normal epidermal keratinocytes using Mouse Anti-Human Cytokeratin 14 Monoclonal Antibody (Catalog # MAB3164) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007). CKAP4/p63 was also detected using Sheep Anti-Human CKAP4/p63 Affinity-purified Polyclonal Antibody (Catalog # AF7355) and stained using the NorthernLights™ 493-conjugated Anti-Sheep IgG Secondary Antibody (green; Catalog # NL012). Cells were counterstained with DAPI (blue). Specific staining of Cytokeratin 14 was localized to intermediate filaments. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cytokeratin 14
Cytokeratin 14, a type I intermediate filament protein, is one of the
two keratins which distinguish stratifying epithelium from simple
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