hh Human Cytokeratin 14 Antibody MAB3164: R&D Systems

Human Cytokeratin 14 Antibody

(1 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human Cytokeratin 14 in Western blots.
  • Source
    Monoclonal Mouse IgG2A Clone # LL001
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Human Cytokeratin 14 synthetic peptide
    DGKVVSTHEQVLRTKN
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot

    Purkis, P. et al. (1990) J. Cell Sci. 97:39. This application was not tested by R&D Systems.

  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry

Cytokeratin 14 in NHEK Human Normal Epidermal Keratinocytes. Cytokeratin 14 was detected in immersion fixed NHEK human normal epidermal keratinocytes using Mouse Anti-Human Cytokeratin 14 Monoclonal Antibody (Catalog # MAB3164) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007). CKAP4/p63 was also detected using Sheep Anti-Human CKAP4/p63 Affinity-purified Polyclonal Antibody (Catalog # AF7355) and stained using the NorthernLights™ 493-conjugated Anti-Sheep IgG Secondary Antibody (green; Catalog # NL012). Cells were counterstained with DAPI (blue). Specific staining of Cytokeratin 14 was localized to intermediate filaments. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cytokeratin 14
Cytokeratin 14, a type I intermediate filament protein, is one of the two keratins which distinguish stratifying epithelium from simple epithelium.
  • Entrez Gene IDs:
    3861 (Human)
  • Alternate Names:
    CK14; CK-14; Cytokeratin 14; cytokeratin-14; EBS3; EBS4; K14; keratin 14 (epidermolysis bullosa simplex, Dowling-Meara, Koebner); keratin 14; keratin, type I cytoskeletal 14; keratin-14; KRT14; NFJ
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Decreased IGF type 1 receptor signaling in mammary epithelium during pregnancy leads to reduced proliferation, alveolar differentiation, and expression of insulin receptor substrate (IRS)-1 and IRS-2.
    Authors: Sun Z, Shushanov S, LeRoith D, Wood TL
    Endocrinology, 2011;152(8):3233-45.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
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Anti-Mouse HRP-DAB Cell & Tissue Staining Kit

CTS002 14
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Isotype Controls
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Mouse IgG2A Isotype Control

Ctrl MAB003 32  
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Mouse IgG2A Isotype Control

Ctrl MAB0031 7  
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Mouse IgG2A Isotype Control

Ctrl MAB003R
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Secondary Antibodies
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Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse IgG Antibody

WB AF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Rat Anti-Mouse IgG2A PE-conjugated Antibody

Flow F0129 1  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Rat Anti-Mouse IgG2A APC-conjugated Antibody

Flow F0130  
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Rat Anti-Mouse IgG2A PerCP-conjugated Antibody

Flow F0131  
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Mouse IgG2A Antibody

Flow MAB0032  
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