Human Cytokeratin 14 Antibody

Catalog # Availability Size / Price Qty
MAB3164
MAB3164-SP
Cytokeratin 14 in A431 Human Cell Line.
3 Images
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews (3)

Human Cytokeratin 14 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Cytokeratin 14 in Western blots.
Source
Monoclonal Mouse IgG2A Clone # LL001
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human Cytokeratin 14 synthetic peptide
DGKVVSTHEQVLRTKN
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

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Applications

Recommended Concentration
Sample
Western Blot

Purkis, P. et al. (1990) J. Cell Sci. 97:39. This application was not tested by R&D Systems.

 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry View Larger

Cytokeratin 14 in A431 Human Cell Line. Cytokeratin 14 was detected in immersion fixed A431 human epithelial carcinoma cell line using Mouse Anti-Human Cytokeratin 14 Monoclonal Antibody (Catalog # MAB3164) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunocytochemistry Cytokeratin 14 antibody in NHEK Human Normal Epidermal Keratinocytes by Immunocytochemistry (ICC). View Larger

Cytokeratin 14 in NHEK Human Normal Epidermal Keratinocytes. Cytokeratin 14 was detected in immersion fixed NHEK human normal epidermal keratinocytes using Mouse Anti-Human Cytokeratin 14 Monoclonal Antibody (Catalog # MAB3164) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007). CKAP4/p63 was also detected using Sheep Anti-Human CKAP4/p63 Affinity-purified Polyclonal Antibody (Catalog # AF7355) and stained using the NorthernLights™ 493-conjugated Anti-Sheep IgG Secondary Antibody (green; Catalog # NL012). Cells were counterstained with DAPI (blue). Specific staining of Cytokeratin 14 was localized to intermediate filaments. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry View Larger

Detection of Cytokeratin 14 in human skin. Cytokeratin 14 was detected in immersion fixed paraffin-embedded sections of human skin using Mouse Anti-Human Cytokeratin 14 Monoclonal Antibody (Catalog # MAB3164) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to keratinocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cytokeratin 14

Cytokeratin 14, a type I intermediate filament protein, is one of the two keratins which distinguish stratifying epithelium from simple epithelium.

Entrez Gene IDs
3861 (Human)
Alternate Names
CK14; CK-14; Cytokeratin 14; cytokeratin-14; EBS3; EBS4; K14; keratin 14 (epidermolysis bullosa simplex, Dowling-Meara, Koebner); keratin 14; keratin, type I cytoskeletal 14; keratin-14; KRT14; NFJ

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Citation for Human Cytokeratin 14 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Decreased IGF type 1 receptor signaling in mammary epithelium during pregnancy leads to reduced proliferation, alveolar differentiation, and expression of insulin receptor substrate (IRS)-1 and IRS-2.
    Authors: Sun Z, Shushanov S, LeRoith D, Wood TL
    Endocrinology, 2011;152(8):3233-45.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P

FAQs

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Immunohistochemistry Reagents

Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

Reviews for Human Cytokeratin 14 Antibody

Average Rating: 4 (Based on 3 Reviews)

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Human Cytokeratin 14 Antibody
By Anonymous on 01/14/2022
Application: ICC/IF Sample Tested: fibroblasts Species: Human

Human Cytokeratin 14 Antibody
By Agnès Tessier on 02/27/2020
Application: ICC/IF Sample Tested: Skin tissue Species: Human

Anti-keratin 14 antibody was tested at 1:50 and 1:100 dilutions and the protocol includes citrate antigen retrieval. Pepsin and pronase were also used as alternative methods for antigen retrieval, without success. The antibody is not specific of the basal layer of the skin and targets all the layers.


Human Cytokeratin 14 Antibody
By Agnès Tessier on 06/17/2019
Application: Simple Western Sample Tested: Skin tissue Species: Human

Detection of keratin 14 by Simple Western (JESS) in reconstructed human epidermis lysate (about 200 µg/mL protein concentration).
Antibody dilution: 1/50