Human Dkk-1 Antibody (141135)

R&D Systems | Catalog # MAB10962

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, ELISA Capture (Matched Antibody Pair)

Cited:

Immunohistochemistry, Neutralization, ELISA Development

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 141135
View all Dkk-1 Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Dkk-1
Thr32-His266
Accession # O94907

Specificity

Detects human Dkk-1 in ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant human (rh) Dkk-4, rhKremen-1, rhKremen-2 or rhLRP-6 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Dkk-1 Antibody (141135)

Dkk-1 antibody in Human Colon by Immunohistochemistry (IHC-P).

Dkk‑1 in Human Colon.

Dkk-1 was detected in immersion fixed paraffin-embedded sections of human colon using Human Dkk-1 Monoclonal Antibody (Catalog # MAB10962) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002). Specific staining was localized to epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Human Dkk-1 ELISA Standard Curve

Human Dkk-1 ELISA Standard Curve

Recombinant Human Dkk‑1 (Catalog # 5439-DK) was serially diluted and captured by Mouse Anti-Human Dkk‑1 Monoclonal Antibody (Catalog # MAB10962) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human/Mouse Dkk‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1096) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human Dkk-1 Antibody (141135)

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human colon

Human Dkk-1 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human Dkk‑1 Biotinylated Antibody (Catalog # BAF1096)
  • Standard: Recombinant Human Dkk-1 Protein (Catalog # 5439-DK)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 5 using MAB10962 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Dkk-1

Dkk-1 (Dickkopf related protein 1) is a secreted antagonist of Wnt/beta-Catenin signaling. It interacts with LRP-6, and internalized complexes of Dkk-1/LRP-6/Kremen 2 can downregulate Wnt signaling. The balance between Wnt signaling and Dkk-1 inhibition is critical for bone formation and homeostasis. In addition, Dkk-1 can act as either a tumor suppressor or promoter of metastasis.

Long Name

Dickkopf-1

Alternate Names

Dkk1

Entrez Gene IDs

22943 (Human); 13380 (Mouse); 102123735 (Cynomolgus Monkey)

Gene Symbol

DKK1

UniProt

O94907

Additional Dkk-1 Products

Product Documents for Human Dkk-1 Antibody (141135)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Dkk-1 Antibody (141135)

For research use only

Citations for Human Dkk-1 Antibody (141135)

Customer Reviews for Human Dkk-1 Antibody (141135) (2)

5 out of 5
2 Customer Ratings
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  • Human Dkk-1 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Brain tissue
    Species: Human
    Verified Customer | Posted 10/21/2021
    Human Dkk-1 Antibody (141135) MAB10962
  • Human Dkk-1 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Recombinant protein
    Species: Human
    Verified Customer | Posted 12/17/2020
    Human Dkk-1 Antibody (141135) MAB10962

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