Human E-Cadherin Antibody

(5 citations)
(3 Reviews)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human E-Cadherin in direct ELISAs.
  • Source
    Monoclonal Mouse IgG2B Clone # 180224
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human E-Cadherin
    Asp155-Ile707
    Accession # P12830
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
  • Flow Cytometry
    0.25 µg/106 cells
    MCF‑7 human breast cancer cell line stained in buffer containing Ca2+ and Mg2+
  • Immunohistochemistry
    8-25 µg/mL
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    3-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human E‑Cadherin by Western Blot. Western blot shows lysates of MCF‑7 human breast cancer cell line, BT‑20 human breast cancer cell line, and T47D human breast cancer cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human E‑Cadherin Monoclonal Antibody (Catalog # MAB18381) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for E‑Cadherin at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
E‑Cadherin in MCF‑7 Human Cell Line. E‑Cadherin was detected in immersion fixed MCF‑7 human breast cancer cell line using Mouse Anti-Human E‑Cadherin Monoclonal Antibody (Catalog # MAB18381) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunohistochemistry
E‑Cadherin in Human Stomach.
E‑Cadherin was detected in immersion fixed paraffin-embedded sections of human stomach using Mouse Anti-Human E‑Cadherin Monoclonal Antibody (Catalog # MAB18381) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Immunohistochemistry
E‑Cadherin in Human Skin. E‑Cadherin was detected in immersion fixed paraffin-embedded sections of human skin using Mouse Anti-Human E‑Cadherin Monoclonal Antibody (Catalog # MAB18381) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: E-Cadherin

Epithelial (E)‑Cadherin (ECAD), also known as Cadherin-1, cell-CAM120/80 in the human, uvomorulin in the mouse, Arc-1 in the dog, and L-CAM in the chicken, is a member of the Cadherin family of cell adhesion molecules (gene name CDH1). Cadherins are calcium-dependent transmembrane proteins which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha, beta, and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. E-Cadherin may also play a role in tumor development, as loss of E-Cadherin has been associated with tumor invasiveness. E-Cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium‑dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. E‑Cadherin contains five extracellular calcium-binding domains of approximately 110 amino acids each (amino acids 155-697).

  • References:
    1. Bussemakers, M.J.G. et al. (1993) Mol. Biol. Reports 17:123.
    2. Overduin, M. et al. (1995) Science 267:386.
    3. Takeichi, M. (1991) Science 251:1451.
  • Entrez Gene IDs:
    999 (Human); 12550 (Mouse); 83502 (Rat)
  • Alternate Names:
    Arc-1; CAD1; cadherin 1, E-cadherin (epithelial); cadherin 1, type 1, E-cadherin (epithelial); Cadherin-1; calcium-dependent adhesion protein, epithelial; CAM 120/80; CD324 antigen; CD324; CDH1; CDHE; cell-CAM 120/80; Cell-CAM120/80; ECAD; ECadherin; E-Cadherin; Epithelial cadherin; LCAM; L-CAM; UVOE-Cadherin; Uvomorulin
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Species
Applications
Sample Type
  1. Semaphorin 3A induces mesenchymal-stem-like properties in human periodontal ligament cells.
    Authors: Wada, Naohisa, Maeda, Hidefumi, Hasegawa, Daigaku, Gronthos, Stan, Bartold, Peter Ma, Menicanin, Danijela, Fujii, Shinsuke, Yoshida, Shinichi, Tomokiyo, Atsushi, Monnouchi, Satoshi, Akamine, Akifumi
    Stem Cells Dev, 2014;23(18):2225-36.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC Fresh
  2. Generation of glucose-responsive, insulin-producing cells from human umbilical cord blood-derived mesenchymal stem cells.
    Authors: Prabakar K, Dominguez-Bendala J, Molano R, Pileggi A, Villate S, Ricordi C, Inverardi L
    Cell Transplant, 2012;21(6):1321-39.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC Fluorescence
  3. Gram-positive bacteria enhance HIV-1 susceptibility in Langerhans cells, but not in dendritic cells, via Toll-like receptor activation.
    Authors: Ogawa Y, Kawamura T, Kimura T, Ito M, Blauvelt A, Shimada S
    Blood, 2009;113(21):5157-66.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  4. Generation of pluripotent stem cells from adult human testis.
    Authors: Conrad S, Renninger M, Hennenlotter J, Wiesner T, Just L, Bonin M, Aicher W, Buhring HJ, Mattheus U, Mack A, Wagner HJ, Minger S, Matzkies M, Reppel M, Hescheler J, Sievert KD, Stenzl A, Skutella T
    Nature, 2008;456(7220):344-9.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  5. Expression of mesenchyme-specific gene HMGA2 in squamous cell carcinomas of the oral cavity.
    Authors: Miyazawa J, Mitoro A, Kawashiri S, Chada KK, Imai K
    Cancer Res., 2004;64(6):2024-9.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
Cell and Tissue Staining Kits
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Isotype Controls
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Mouse IgG2B Isotype Control

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Mouse IgG2B Isotype Control

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Secondary Antibodies
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Mouse IgG HRP-conjugated Antibody

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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

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Donkey Anti-Mouse IgG Biotinylated Antibody

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Mouse IgG HRP-conjugated Antibody

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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

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Mouse IgG Antibody

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Goat Anti-Mouse IgG Biotinylated Antibody

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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

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Rat Anti-Mouse IgG2B APC-conjugated Antibody

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Mouse IgG VisUCyte HRP Polymer Antibody

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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

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Rat Anti-Mouse IgG2B Fluorescein-conjugated Antibody

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Rat Anti-Mouse IgG2B PE-conjugated Antibody

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Mouse IgG2B Antibody

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Average Rating: 4 (Based on 3 reviews)

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We have 3 reviews tested in 1 species: Human.
We have 3 reviews tested in 3 applications: Immunocytochemistry/Immunofluorescence, Western Blot, Flow Cytometry.

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Images Ratings Applications Species Reviewed By Date Details
Immunocytochemistry/Immunofluorescence Human E-Cadherin Antibody MAB18381
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 ICC/IF Human Anonymous 10/30/2017
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Immunocytochemistry/Immunofluorescence Human E-Cadherin Antibody MAB18381
Immunocytochemistry/Immunofluorescence: Human E-Cadherin Antibody [MAB18381]

Summary

ApplicationImmunocytochemistry/Immunofluorescence
Sample TestedRT4 Urinary bladder transitional cell carcinoma,RT4 Urinary bladder transitional cell carcinoma cell line
SpeciesHuman
Western Blot Human E-Cadherin Antibody MAB18381
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Excellent
 WB Human Anonymous 12/04/2016
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Western Blot Human E-Cadherin Antibody MAB18381
Western Blot: Human E-Cadherin Antibody [MAB18381]

Summary

ApplicationWestern Blot
Sample TestedCell Lysates
SpeciesHuman

Other Experimental Details

Other Experimental DetailsExpression of E-cadherin in different breast cancer cell lines.
Flow Cytometry Human E-Cadherin Antibody MAB18381
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Very Good
 Flow Human Anonymous 07/19/2016
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Flow Cytometry Human E-Cadherin Antibody MAB18381
Flow Cytometry: Human E-Cadherin Antibody [MAB18381]

Summary

ApplicationFlow Cytometry
Sample Tested3T3-L1 mouse embryonic fibroblast adipose-like cell line
SpeciesHuman

Other Experimental Details

Other Experimental DetailsScreen for the transfectant cells.

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