Key Product Details

Species Reactivity

Human

Applications

ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG2B Clone # 10043R
View all FGF basic/FGF2/bFGF Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human FGF basic/FGF2/bFGF
Accession # P09038

Specificity

Detects human FGF basic/FGF2/bFGF in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human FGF basic/FGF2/bFGF Antibody

Human FGF basic/FGF2/bFGF Antibody in ELISA Standard Curve.

Human FGF basic/FGF2/bFGF ELISA Standard Curve.

Recombinant Human FGF basic/FGF2/bFGF protein was serially diluted 2-fold and captured by Mouse Anti-Human FGF basic/FGF2/bFGF Monoclonal Antibody(Catalog # MAB233R) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human FGF basic/FGF2/bFGF Monoclonal Antibody (Catalog # MAB2331) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Applications for Human FGF basic/FGF2/bFGF Antibody

Application
Recommended Usage

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human FGF basic/FGF2/bFGF Monoclonal Antibody (Catalog # MAB233R).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human FGF basic/FGF2/bFGF DuoSet ELISA Kit (Catalog # DY233) for convenient development of a sandwich ELISA or the Human FGF basic/FGF2/bFGF Quantikine ELISA Kit (Catalog # DFB50) for a complete optimized ELISA.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FGF basic/FGF2/bFGF

FGF basic, also known as FGF-2, is a member of the FGF family of growth factors.

Long Name

Fibroblast Growth Factor basic

Alternate Names

bFGF, FGF-2, FGF2, HBGF-2, Prostatropin

Entrez Gene IDs

2247 (Human); 14173 (Mouse); 281161 (Bovine); 403857 (Canine); 100033955 (Equine)

Gene Symbol

FGF2

UniProt

P09038

Additional FGF basic/FGF2/bFGF Products

Product Documents for Human FGF basic/FGF2/bFGF Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human FGF basic/FGF2/bFGF Antibody

For research use only

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