Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 1019104
View all FOXL2 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide containing human FOXL2
Accession # P58012

Specificity

Detects human FOXL2 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human FOXL2 Antibody

FOXL2 antibody in PANC-1 Human Cell Line by Immunocytochemistry (ICC).

FOXL2 in PANC-1 Human Cell Line.

FOXL2 was detected in immersion fixed PANC-1 human pancreatic carcinoma cell line (positive) and MCF-7 human breast cancer cell line (negative) using Mouse Anti-Human FOXL2 Monoclonal Antibody (Catalog # MAB10396) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
FOXL2 antibody in Ovarian Cancer Tissue by Immunohistochemistry (IHC-P).

FOXL2 in Ovarian Cancer Tissue.

FOXL2 was detected in immersion fixed paraffin-embedded sections of ovarian cancer tissue using Mouse Anti-Human FOXL2 Monoclonal Antibody (Catalog # MAB10396) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human FOXL2 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed PANC-1 human pancreatic carcinoma cell line (positive) and MCF‑7 human breast cancer cell line (negative) 

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of ovarian cancer tissue

Reviewed Applications

Read 3 reviews rated 4.7 using MAB10396 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FOXL2

FOXL2 is a forkhead transcription factor which plays a role in ovarian development and function. It is transcribed very early in somatic cells of the developing gonad (before sex determination) and its expression persists in the follicular cells of the adult ovary. Females missing the FOXL2 gene appear to be male.  FOXL2 prevents transdifferentiation of an adult ovary to a testis.  Overexpression of FOXL2 has been implicated in endometriosis with 3-fold higher mRNA expression in endometriosis than in healthy endometrium.

Long Name

Forkhead Box L2

Alternate Names

BPES, BPES1, PFRK, PINTO, POF3

Entrez Gene IDs

668 (Human); 26927 (Mouse)

Gene Symbol

FOXL2

UniProt

P58012

Additional FOXL2 Products

Product Documents for Human FOXL2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human FOXL2 Antibody

For research use only

Customer Reviews for Human FOXL2 Antibody (3)

4.7 out of 5
3 Customer Ratings
5 Stars
67%
4 Stars
33%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Customer Images

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  • Human FOXL2 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Ovary tissue
    Species: Mouse
    Verified Customer | Posted 12/01/2024
    E18.5 Ovary. IHC-P. 1:100
    Human FOXL2 Antibody MAB10396
    Bio-Techne Response
    This review reflects a new species or application tested on a primary antibody.
  • Human FOXL2 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Ovary (follicle with oocyte)
    Species: Mouse
    Verified Customer | Posted 11/28/2024
    Concentration 1:100, paraffin section
    Human FOXL2 Antibody MAB10396
    Bio-Techne Response
    This review reflects a new species or application tested on a primary antibody.
  • Human FOXL2 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Ovary tissue
    Species: Human
    Verified Customer | Posted 02/24/2022
    Human FOXL2 Antibody MAB10396

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Protocols

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