Human Galectin-3 Antibody

(5 citations)
(1 Review)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human Galectin-3 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse Galectin-3 is observed and less than 1% cross-reactivity with recombinant human (rh) Galectin-1, rhGalectin-2, rhGalectin-4, rhGalectin-7, and rhGalectin-8 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human Galectin‑3
    Ala2-Ile250
    Accession # P17931.5
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    See below
  • Simple Western
    10 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Intracellular Staining by Flow Cytometry
    2.5 µg/106 cells
    Human PBMCs fixed with paraformaldehyde and permeabilized with saponin
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Galectin‑3 by Western Blot. Western blot shows lysates of COLO 205 human colorectal adenocarcinoma cell line, MCF‑7 human breast cancer cell line, and U‑118‑MG human glioblastoma/astrocytoma cell line. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human Galectin‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1154) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Galectin‑3 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
Galectin‑3 in Human Colon. Galectin‑3 was detected in formalin fixed paraffin-embedded sections of human colon using Goat Anti-Human Galectin‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1154) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of immersion fixed paraffin-embedded Tissue Sections.
Detection of Human Galectin‑3 by Simple WesternTM. Simple Western lane view shows lysates of COLO 205 human colorectal adenocarcinoma cell line, MCF‑7 human breast cancer cell line, and U‑118‑MG human glioblastoma/astrocytoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Galectin‑3 at approximately 37-38 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Galectin‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1154) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Galectin-3
The galectins constitute a large family of carbohydrate-binding proteins with specificity for N-acetyl-lactosamine-containing glycoproteins. At least 14 mammalian galectins, which share structural similarities in their carbohydrate recognition domains (CRD), have been identified. The galectins have been classified into the prototype galectins (-1, -2, -5, -7, -10, -11, -13, -14), which contain one CRD and exist either as a monomer or a noncovalent homodimer; the chimera galectins (Galectin-3) containing one CRD linked to a nonlectin domain; and the tandem-repeat galectins (-4, -6, -8, -9, -12) consisting of two CRDs joined by a linker peptide. Galectins lack a classical signal peptide and can be localized to the cytosolic compartments where they have intracellular functions. However, via one or more as yet unidentified non-classical secretory pathways, galectins can also be secreted to function extracellularly. Individual members of the galectin family have different tissue distribution profiles and exhibit subtle differences in their carbohydrate-binding specificities. Each family member may preferentially bind to a unique subset of cell-surface glycoproteins (1-4). Galectin-3, also known as Mac-2, L29, CBP35, and epsilon BP, is a chimera galectin that has a tendency to dimerize. Besides the soluble protein, alternatively spliced forms of chicken Galectin-3 containing a transmembrane-spanning domain and a leucine zipper motif have been reported. Galectin-3 is expressed in tumor cells, macrophages, activated T cells, osteoclasts, epithelial cells, and fibroblasts. It binds various matrix glycoproteins including laminin, fibronectin, LAMPS, 90K/Mac-2BP, MP20, and CEA. Galectin-3 promotes cell growth and proliferation for many cell types. Galectin-3 acts intracellularly to prevent apoptosis. Depending on the cell types, Galectin-3 exhibits pro- or anti-adhesive properties. Galectin-3 has proinflammatory activities in vitro and in vivo. It induces pro-inflammatory and inhibits Th2 type cytokine production. Galectin-3 chemoattracts monocytes and macrophages. It activates and degranulates basophils and mast cells. Elevated circulating levels of Galectin-3 has been show to correlate with the malignant potential of several types of cancer, suggesting that Galectin-3 is also involved in tumor growth and metastasis. Human and mouse Galectin-3 shares approximately 80% amino acid sequence similarity (1-5).
  • References:
    1. Rabinovich, A. et al. (2002) Trends in Immunol. 23:313.
    2. Rabinovich, A. et al. (2002) J. Leukocyte Biology 71:741.
    3. Hughes, R.C. (2001) Biochimie 83:667.
    4. R&D Systems Cytokine Bulletin; Summer 2002.
    5. Gorski, J.P. et al. (2002) J. Biol. Chem. 277:18840.
  • Entrez Gene IDs:
    3958 (Human); 16854 (Mouse)
  • Alternate Names:
    AGE-R3; Carbohydrate-binding protein 35; CBP 35,35 kDa lectin; CBP35; GAL3; Gal-3; galactin-3; Galactose-specific lectin 3; Galactoside-binding protein; GALBPCBP35; Galectin3; Galectin-3; GALIG; IgE-binding protein; L29; L31; L-31; Laminin-binding protein; Lectin L-29; lectin, galactoside-binding, soluble, 3; LGALS2; LGALS3; Mac-2 antigen; Mac-2; MAC2GAL3
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Applications
Sample Type
  1. Intranuclear accumulation of galectin-3 is an independent prognostic factor for patients with distal cholangiocarcinoma
    Authors: T Shimura, Y Kofunato, R Okada, R Yashima, Y Koyama, K Araki, H Kuwano, S Takenoshit
    Oncol Lett, 2017;14(1):819-829.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  2. Tonsil-derived mesenchymal stem cells alleviate concanavalin A-induced acute liver injury.
    Authors: Ryu K, Kim S, Kim Y, Woo S, Sung S, Kim H, Jung S, Jo I, Park J
    Exp Cell Res, 2014;326(1):143-54.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  3. Modulation of endothelial cell migration and angiogenesis: a novel function for the "tandem-repeat" lectin galectin-8.
    Authors: Delgado VM, Nugnes LG, Colombo LL, Troncoso MF, Fernandez MM, Malchiodi EL, Frahm I, Croci DO, Compagno D, Rabinovich GA, Wolfenstein-Todel C, Elola MT
    FASEB J., 2011;25(1):242-54.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  4. The galectin profile of the endothelium: altered expression and localization in activated and tumor endothelial cells.
    Authors: Thijssen VL, Hulsmans S, Griffioen AW
    Am. J. Pathol., 2008;172(2):545-53.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  5. Sialylation of beta1 integrins blocks cell adhesion to galectin-3 and protects cells against galectin-3-induced apoptosis.
    Authors: Zhuo Y, Chammas R, Bellis SL
    J. Biol. Chem., 2008;283(32):22177-85.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
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Normal Goat IgG Control

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Secondary Antibodies
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Average Rating: 5 (Based on 1 review)

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We have 1 review tested in 1 application: Immunocytochemistry/Immunofluorescence.

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Images Ratings Applications Species Reviewed By Date Details
Immunocytochemistry/Immunofluorescence Human Galectin-3 Antibody AF1154
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 ICC/IF Mouse Anonymous 11/18/2016
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Immunocytochemistry/Immunofluorescence Human Galectin-3 Antibody AF1154
Immunocytochemistry/Immunofluorescence: Human Galectin-3 Antibody [AF1154]

Summary

ApplicationImmunocytochemistry/Immunofluorescence
Sample TestedRAW 264.7 mouse monocyte/macrophage cell line
SpeciesMouse

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