Human Galectin-3 Antibody
Human Galectin-3 Antibody Summary
Ala2-Ile250
Accession # P17931.5
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
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Detection of Human Galectin‑3 by Western Blot. Western blot shows lysates of COLO 205 human colorectal adenocarcinoma cell line, MCF-7 human breast cancer cell line, and U-118-MG human glioblastoma/astrocytoma cell line. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human Galectin-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1154) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for Galectin-3 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Galectin‑3 in Human Colon. Galectin-3 was detected in formalin fixed paraffin-embedded sections of human colon using Goat Anti-Human Galectin-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1154) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; (CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of immersion fixed paraffin-embedded Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Galectin-3
The galectins constitute a large family of carbohydrate-binding proteins with specificity for N-acetyl-lactosamine-containing glycoproteins. At least 14 mammalian galectins, which share structural similarities in their carbohydrate recognition domains (CRD), have been identified. The galectins have been classified into the prototype galectins (-1, -2, -5, -7, -10, -11, -13, -14), which contain one CRD and exist either as a monomer or a noncovalent homodimer; the chimera galectins (Galectin-3) containing one CRD linked to a nonlectin domain; and the tandem-repeat galectins (-4, -6, -8, -9, -12) consisting of two CRDs joined by a linker peptide. Galectins lack a classical signal peptide and can be localized to the cytosolic compartments where they have intracellular functions. However, via one or more as yet unidentified non-classical secretory pathways, galectins can also be secreted to function extracellularly. Individual members of the galectin family have different tissue distribution profiles and exhibit subtle differences in their carbohydrate-binding specificities. Each family member may preferentially bind to a unique subset of cell-surface glycoproteins (1-4). Galectin-3, also known as Mac-2, L29, CBP35, and epsilon BP, is a chimera galectin that has a tendency to dimerize. Besides the soluble protein, alternatively spliced forms of chicken Galectin-3 containing a transmembrane-spanning domain and a leucine zipper motif have been reported. Galectin-3 is expressed in tumor cells, macrophages, activated T cells, osteoclasts, epithelial cells, and fibroblasts. It binds various matrix glycoproteins including laminin, fibronectin, LAMPS, 90K/Mac-2BP, MP20, and CEA. Galectin-3 promotes cell growth and proliferation for many cell types. Galectin-3 acts intracellularly to prevent apoptosis. Depending on the cell types, Galectin-3 exhibits pro- or anti-adhesive properties. Galectin-3 has proinflammatory activities in vitro and in vivo. It induces pro-inflammatory and inhibits Th2 type cytokine production. Galectin-3 chemoattracts monocytes and macrophages. It activates and degranulates basophils and mast cells. Elevated circulating levels of Galectin-3 has been show to correlate with the malignant potential of several types of cancer, suggesting that Galectin-3 is also involved in tumor growth and metastasis. Human and mouse Galectin-3 shares approximately 80% amino acid sequence similarity (1-5).
- Rabinovich, A. et al. (2002) Trends in Immunol. 23:313.
- Rabinovich, A. et al. (2002) J. Leukocyte Biology 71:741.
- Hughes, R.C. (2001) Biochimie 83:667.
- R&D Systems Cytokine Bulletin; Summer 2002.
- Gorski, J.P. et al. (2002) J. Biol. Chem. 277:18840.
Product Datasheets
Citations for Human Galectin-3 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 8
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Galectin-3 deficiency in pregnancy increases the risk of fetal growth restriction (FGR) via placental insufficiency
Authors: N Freitag, I Tirado-Gon, G Barrientos, KL Powell, P Boehm-Stur, SP Koch, K Hecher, AC Staff, PC Arck, A Diemert, SM Blois
Cell Death Dis, 2020;11(7):560.
Species: Human
Sample Types: Serum
Applications: ELISA Capture -
Network analysis of the progranulin-deficient mouse brain proteome reveals pathogenic mechanisms shared in human frontotemporal dementia caused by GRN mutations
Authors: M Huang, E Modeste, E Dammer, P Merino, G Taylor, DM Duong, Q Deng, CJ Holler, M Gearing, D Dickson, NT Seyfried, T Kukar
Acta Neuropathol Commun, 2020;8(1):163.
Species: Human
Sample Types: Protein
Applications: Western Blot -
Human trophoblast requires galectin-3 for cell migration and invasion
Authors: Ž Boji?-Trbo, M Jovanovi?, A Viloti?, N Kolundži?, I Stefanoska, F Zetterberg, UJ Nilsson, H Leffler, L Vi?ovac
Sci Rep, 2019;9(1):2136.
Species: Human
Sample Types: Basement Membrane Extract
Applications: ELISA Development (Detection) -
Intranuclear accumulation of galectin-3 is an independent prognostic factor for patients with distal cholangiocarcinoma
Authors: T Shimura, Y Kofunato, R Okada, R Yashima, Y Koyama, K Araki, H Kuwano, S Takenoshit
Oncol Lett, 2017;14(1):819-829.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Tonsil-derived mesenchymal stem cells alleviate concanavalin A-induced acute liver injury.
Authors: Ryu K, Kim S, Kim Y, Woo S, Sung S, Kim H, Jung S, Jo I, Park J
Exp Cell Res, 2014;326(1):143-54.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Modulation of endothelial cell migration and angiogenesis: a novel function for the "tandem-repeat" lectin galectin-8.
Authors: Delgado VM, Nugnes LG, Colombo LL, Troncoso MF, Fernandez MM, Malchiodi EL, Frahm I, Croci DO, Compagno D, Rabinovich GA, Wolfenstein-Todel C, Elola MT
FASEB J., 2011;25(1):242-54.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
The galectin profile of the endothelium: altered expression and localization in activated and tumor endothelial cells.
Authors: Thijssen VL, Hulsmans S, Griffioen AW
Am. J. Pathol., 2008;172(2):545-53.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Sialylation of beta1 integrins blocks cell adhesion to galectin-3 and protects cells against galectin-3-induced apoptosis.
Authors: Zhuo Y, Chammas R, Bellis SL
J. Biol. Chem., 2008;283(32):22177-85.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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