Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot, ELISA Capture (Matched Antibody Pair), Simple Western

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 194805
View all Galectin-3 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Galectin‑3
Ala2-Ile250
Accession # P17931.5

Specificity

Detects human Galectin-3 in ELISAs. In sandwich immunoassays, no cross-reactivity with recombinant human Galectin-1, 2, 4, 7, 8, or 10 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Galectin‑3 Antibody

Detection of Human Galectin‑3 antibody by Western Blot.

Detection of Human Galectin‑3 by Western Blot.

Western blot shows lysates of COLO 205 human colorectal adenocarcinoma cell line, MCF-7 human breast cancer cell line, and U-118-MG human glioblastoma/astrocytoma cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human Galectin-3 Monoclonal Antibody (Catalog # MAB11541) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Galectin-3 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Galectin-3 antibody in Human Prostate Cancer Tissue by Immunohistochemistry (IHC-P).

Galectin‑3 in Human Prostate Cancer Tissue.

Galectin-3 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Mouse Anti-Human Galectin-3 Monoclonal Antibody (Catalog # MAB11541) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membrane in epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Detection of Human Galectin-3 antibody by Simple WesternTM.

Detection of Human Galectin‑3 by Simple WesternTM.

Simple Western lane view shows lysates of COLO 205 human colorectal adenocarcinoma cell line, MCF‑7 human breast cancer cell line, and U‑118‑MG human glioblastoma/astrocytoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Galectin‑3 at approximately 38 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Galectin‑3 Monoclonal Antibody (Catalog # MAB11541). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Applications for Human Galectin‑3 Antibody

Application
Recommended Usage

Immunohistochemistry

1-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human prostate cancer tissue

Simple Western

10 µg/mL
Sample: COLO 205 human colorectal adenocarcinoma cell line, MCF‑7 human breast cancer cell line, and U‑118‑MG human glioblastoma/astrocytoma cell line

Western Blot

0.2 µg/mL
Sample: COLO 205 human colorectal adenocarcinoma cell line, MCF‑7 human breast cancer cell line, and U‑118‑MG human glioblastoma/astrocytoma cell line

Human Galectin-3 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human Galectin‑3 Biotinylated Antibody (Catalog # BAF1154)
  • Standard: Recombinant Human Galectin-3 Protein (Catalog # 1154-GA)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 4 reviews rated 4.5 using MAB11541 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Galectin-3

Galectin-3, also known as Mac-2, L29, CBP35 and epsilon BP, is a member of a large family of carbohydrate-binding proteins with specificity for N-acetyl-lactosamine-containing glycoproteins. At least 14 mammalian galectins, which share structural similarities in their carbohydrate recognition domains (CRD), have been identified to date. Galectin-3 is expressed in tumor cells, macrophages, activated T cells, osteoclasts, epithelial cells and fibroblasts.

Alternate Names

AGE-R3, CBP35, GAL3, Galectin3, L29, LGALS3, Mac-2

Entrez Gene IDs

3958 (Human); 16854 (Mouse)

Gene Symbol

LGALS3

UniProt

P17931.5

Additional Galectin-3 Products

Product Documents for Human Galectin‑3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Galectin‑3 Antibody

For research use only

Citations for Human Galectin‑3 Antibody

Customer Reviews for Human Galectin‑3 Antibody (4)

4.5 out of 5
4 Customer Ratings
5 Stars
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Showing  1 - 4 of 4 reviews Showing All
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  • Human Galectin‑3 Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Human cell line
    Species: Human
    Verified Customer | Posted 10/26/2015
    Galectin-3 is detectable on the surface of the cells and is suitable at lower dilutions also. <br />Specificity: Specific<br />Sensitivity: Sensitive<br />Buffer: PBS<br />Dilution: 1:1000
    Human Galectin‑3 Antibody MAB11541
  • Human Galectin‑3 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Cancer Tissue
    Species: Human
    Verified Customer | Posted 10/26/2015
    The antibody is specific and detects only cells but not the background. <br />Specificity: Specific<br />Sensitivity: Sensitive<br />Buffer: PBS<br />Dilution: 1:1000
    Human Galectin‑3 Antibody MAB11541
  • Human Galectin‑3 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Colon cancer cell line
    Species: Human
    Verified Customer | Posted 10/26/2015
    Very good binding ability. Detects galectin-3 at low dilutions also. <br />Specificity: Reasonably specific<br />Sensitivity: Sensitive<br />Buffer: PBS<br />Dilution: 1:1000
    Human Galectin‑3 Antibody MAB11541
  • Human Galectin‑3 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Cell lysate from colon cancer cell line
    Verified Customer | Posted 10/26/2015
    Very sensitive, followed Milk block, primary BSA, Secondary Milk. As low as 1:2500 works too. <br />Specificity: Specific<br />Sensitivity: Sensitive<br />Buffer: Milk, BSA, Milk<br />Dilution: 1:1000
    Human Galectin‑3 Antibody MAB11541

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Protocols

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