Human Galectin-3 Antibody

Catalog # Availability Size / Price Qty
MAB11541-SP
MAB11541-500
MAB11541-100
Detection of Human Galectin‑3 by Western Blot.
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Product Details
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Reviews (4)

Human Galectin-3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Galectin-3 in ELISAs. In sandwich immunoassays, no cross-reactivity with recombinant human Galectin-1, 2, 4, 7, 8, or 10 is observed.
Source
Monoclonal Mouse IgG2B Clone # 194805
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Galectin‑3
Ala2-Ile250
Accession # P17931.5
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.2 µg/mL
See below
Simple Western
10 µg/mL
See below
Immunohistochemistry
1-25 µg/mL
See below

Human Galectin-3 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human Galectin‑3 Biotinylated Antibody (Catalog # BAF1154)

Standard: Recombinant Human Galectin-3 Protein (Catalog # 1154-GA)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human Galectin‑3 by Western Blot. View Larger

Detection of Human Galectin‑3 by Western Blot. Western blot shows lysates of COLO 205 human colorectal adenocarcinoma cell line, MCF‑7 human breast cancer cell line, and U‑118‑MG human glioblastoma/astrocytoma cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human Galectin‑3 Monoclonal Antibody (Catalog # MAB11541) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Galectin‑3 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Galectin‑3  in Human Prostate Cancer Tissue. View Larger

Galectin‑3 in Human Prostate Cancer Tissue. Galectin‑3 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Mouse Anti-Human Galectin‑3 Monoclonal Antibody (Catalog # MAB11541) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membrane in epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Simple Western Detection of Human Galectin‑3 by Simple WesternTM. View Larger

Detection of Human Galectin‑3 by Simple WesternTM. Simple Western lane view shows lysates of COLO 205 human colorectal adenocarcinoma cell line, MCF‑7 human breast cancer cell line, and U‑118‑MG human glioblastoma/astrocytoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Galectin‑3 at approximately 38 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Galectin‑3 Monoclonal Antibody (Catalog # MAB11541). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Galectin-3

Galectin-3, also known as Mac-2, L29, CBP35 and epsilon BP, is a member of a large family of carbohydrate-binding proteins with specificity for N-acetyl-lactosamine-containing glycoproteins. At least 14 mammalian galectins, which share structural similarities in their carbohydrate recognition domains (CRD), have been identified to date. Galectin-3 is expressed in tumor cells, macrophages, activated T cells, osteoclasts, epithelial cells and fibroblasts.

Entrez Gene IDs
3958 (Human); 16854 (Mouse)
Alternate Names
AGE-R3; Carbohydrate-binding protein 35; CBP 35,35 kDa lectin; CBP35; GAL3; Gal-3; galactin-3; Galactose-specific lectin 3; Galactoside-binding protein; GALBPCBP35; Galectin3; Galectin-3; GALIG; IgE-binding protein; L29; L31; L-31; Laminin-binding protein; Lectin L-29; lectin, galactoside-binding, soluble, 3; LGALS2; LGALS3; Mac-2 antigen; Mac-2; MAC2GAL3

Product Datasheets

FAQs

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human Galectin-3 Antibody

Average Rating: 4.5 (Based on 4 Reviews)

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Human Galectin‑3 Antibody
By Anonymous on 10/26/2015
Application: WB Sample Tested: Cell lysate from colon cancer cell line Species: Human

Very sensitive, followed Milk block, primary BSA, Secondary Milk. As low as 1:2500 works too.
Specificity: Specific
Sensitivity: Sensitive
Buffer: Milk, BSA, Milk
Dilution: 1:1000


Human Galectin‑3 Antibody
By Anonymous on 10/26/2015
Application: IHC Sample Tested: Cancer Tissue Species: Human

The antibody is specific and detects only cells but not the background.
Specificity: Specific
Sensitivity: Sensitive
Buffer: PBS
Dilution: 1:1000


Human Galectin‑3 Antibody
By Anonymous on 10/26/2015
Application: ICC/IF Sample Tested: Colon cancer cell line Species: Human

Very good binding ability. Detects galectin-3 at low dilutions also.
Specificity: Reasonably specific
Sensitivity: Sensitive
Buffer: PBS
Dilution: 1:1000


Human Galectin‑3 Antibody
By Anonymous on 10/26/2015
Application: Flow Sample Tested: Human cell line Species: Human

Galectin-3 is detectable on the surface of the cells and is suitable at lower dilutions also.
Specificity: Specific
Sensitivity: Sensitive
Buffer: PBS
Dilution: 1:1000