Human Galectin-3 Antibody
Human Galectin-3 Antibody Summary
Ala2-Ile250
Accession # P17931.5
Applications
Human Galectin-3 Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
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Detection of Human Galectin‑3 by Western Blot. Western blot shows lysates of COLO 205 human colorectal adenocarcinoma cell line, MCF-7 human breast cancer cell line, and U-118-MG human glioblastoma/astrocytoma cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human Galectin-3 Monoclonal Antibody (Catalog # MAB11541) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Galectin-3 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Galectin‑3 in Human Prostate Cancer Tissue. Galectin-3 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Mouse Anti-Human Galectin-3 Monoclonal Antibody (Catalog # MAB11541) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membrane in epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Galectin-3
Galectin-3, also known as Mac-2, L29, CBP35 and epsilon BP, is a member of a large family of carbohydrate-binding proteins with specificity for N-acetyl-lactosamine-containing glycoproteins. At least 14 mammalian galectins, which share structural similarities in their carbohydrate recognition domains (CRD), have been identified to date. Galectin-3 is expressed in tumor cells, macrophages, activated T cells, osteoclasts, epithelial cells and fibroblasts.
Product Datasheets
FAQs
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Reconstitution Buffers
Secondary Antibodies
Reviews for Human Galectin-3 Antibody
Average Rating: 4.5 (Based on 4 Reviews)
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Very sensitive, followed Milk block, primary BSA, Secondary Milk. As low as 1:2500 works too.
Specificity: Specific
Sensitivity: Sensitive
Buffer: Milk, BSA, Milk
Dilution: 1:1000
The antibody is specific and detects only cells but not the background.
Specificity: Specific
Sensitivity: Sensitive
Buffer: PBS
Dilution: 1:1000
Very good binding ability. Detects galectin-3 at low dilutions also.
Specificity: Reasonably specific
Sensitivity: Sensitive
Buffer: PBS
Dilution: 1:1000
Galectin-3 is detectable on the surface of the cells and is suitable at lower dilutions also.
Specificity: Specific
Sensitivity: Sensitive
Buffer: PBS
Dilution: 1:1000
