Human HLA-DR Antibody (1083110)

Name: Human HLA-DR Antibody (1083110)
Brand: R&D Systems
SKU: MAB11555
Price: 169 USD
Availability: InStock

R&D Systems | Catalog # MAB11555

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Multiplex Immunofluorescence, Immunohistochemistry, Western Blot, Simple Western, COMET

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG_2B Clone # 1083110

View all HLA-DR Primary Antibodies »

Product Specifications

Immunogen

Synthetic Peptide
Accession # P01903

Specificity

Detects a synthetic peptide around aa 100 in Direct ELISA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Scientific Data Images for Human HLA-DR Antibody (1083110)

Detection of HLA-DRA in Human Tonsil via seqIF™ staining on COMET™

HLA-DRA Antibody was detected in immersion fixed paraffin-embedded sections of human Tonsil using Mouse Anti-Human HLA-DRA Monoclonal Antibody (Catalog # MAB11555) at 5ug/mL at 32° Celsius for2 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 555 Goat anti-Mouse IgG Secondary Antibody at 1:100 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR555MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.

Detection of HLA-DRA in Human Appendix via seqIF™ staining on COMET™

HLA-DRA Antibody was detected in immersion fixed paraffin-embedded sections of human Appendix using Mouse Anti-Human HLA-DRA Monoclonal Antibody (Catalog # MAB11555) at 5ug/mL at 32° Celsius for2 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 555 Goat anti-Mouse IgG Secondary Antibody at 1:100 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR555MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.

Detection of HLA-DRA in Human Hodgkin Lymphoma via seqIF™ staining on COMET™

HLA-DRA Antibody was detected in immersion fixed paraffin-embedded sections of human Hodgkin Lymphoma using Mouse Anti-Human HLA-DRA Monoclonal Antibody (Catalog # MAB11555) at 5ug/mL at 32° Celsius for2 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 555 Goat anti-Mouse IgG Secondary Antibody at 1:100 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR555MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.

Detection of Human HLA‑DR by Western Blot.

Western Blot shows lysates of HDLM‑2 human Hodgkin’s lymphoma cell line and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 2 µg/ml of Mouse Anti-Human HLA‑DR Monoclonal Antibody (Catalog # MAB11555) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HLA‑DR at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Detection of HLA‑DR in Human Tonsil.

HLA‑DR was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human HLA‑DR Monoclonal Antibody (Catalog # MAB11555) at 1 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human HLA‑DR by Simple Western^TM.

Simple Western shows lysates of HDLM‑2 human Hodgkin’s lymphoma cell line, loaded at 1 mg/ml. A specific band was detected for HLA‑DR at approximately 42 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human HLA‑DR Monoclonal Antibody (Catalog # MAB11555). This experiment was conducted under reducing conditions and using the 12‑230 kDa separation system.

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Applications for Human HLA-DR Antibody (1083110)

Application

Recommended Usage

COMET

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human tonsil

Multiplex Immunofluorescence

1 µg/mL
Sample: Immersion fixed paraffin-embedded sections of Human Tonsil tissue

Simple Western

20 µg/mL
Sample: HDLM-2 human Hodgkin's lymphoma cell line

Western Blot

2 µg/mL
Sample: HDLM-2 human Hodgkin's lymphoma cell line and Raji human Burkitt's lymphoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute lyophilized material at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: HLA-DR

HLA-DR is a transmembrane human major histocompatibility complex 2 (MHC II) family member and consists of a 34 kDa (alpha) subunit and one of several 28 kDa (beta) subunits. HLA-DR is expressed primarily by B cells and dendritic cells (DC), in which it binds peptides derived from internalized and processed antigenic proteins. It presents these peptides on the cell surface for recognition by the T cell receptor on CD4⁺ T cells. This interaction is central to antigen specificity in the adaptive immune response. HLA-DR alleles, polymorphisms, and aberrant expression are linked to a variety of diseases including autoimmunity and cancer.