Human IL-17/IL-17A Antibody

Catalog # Availability Size / Price Qty
MAB3171-SP
MAB3171-100
MAB3171-500
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Detection of IL-17/IL-17A Mouse by Western Blot.
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Product Details
Citations (6)
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Reviews (2)

Human IL-17/IL-17A Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-17 in direct ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant human (rh) IL-17A/IL-17F heterodimer is observed. No cross-reactivity with recombinant mouse IL-17, recombinant canine Il-17, rhIL-17B, rhIL-17C, rhIL-17D, rhIL-17E, or rhIL-17F is observed.
Source
Monoclonal Mouse IgG1 Clone # 41802
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human IL-17
Ile20-Ala155
Accession # Q16552
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Immunohistochemistry
0.5-25 µg/mL
See below
Immunoprecipitation
3 µg/100 µg cell lysate
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of IL-17/IL-17A Mouse antibody by Western Blot. View Larger

Detection of IL-17/IL-17A Mouse by Western Blot. Western blot shows lysates of purified naïve human CD4+T cells orin vitrodifferentiated human Th17 CD4+ T cells. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human IL-17 Monoclonal Antibody (Catalog # MAB3171) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IL-17 at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry IL-17/IL-17A antibody in Human Crohn's Disease Intestine by Immunohistochemistry (IHC-P). View Larger

IL‑17/IL‑17A in Human Crohn's Disease Intestine. IL-17/IL-17A was detected in immersion fixed paraffin-embedded sections of human Crohn's disease intestine using Mouse Anti-Human IL-17/IL-17A Monoclonal Antibody (Catalog # MAB3171) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Immunoprecipitation Immunoprecipitation of Human IL-17. View Larger

Immunoprecipitation of Human IL-17. Human IL-17 was immunoprecipitated from 100 µg of humanin vitrodifferentiated Th17 cell lysate following incubation with 3 µg Mouse Anti-Human IL-17 Monoclonal Antibody (Catalog # MAB3171) or isotype control antibody (Catalog # MAB002) overnight at 4 °C. IL-17-antibody complexes were absorbed using anti-mouse agarose beads. Immunoprecipitated IL-17 was detected by Western blot using 1 µg/mL Goat Anti-Human IL-17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-317-NA). View our recommended buffer recipes for immunoprecipitation.

Intracellular Staining by Flow Cytometry Detection of IL-17 antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of IL‑17 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells were unstimulated (light orange filled histogram) or treated with 50 ng/mL PMA and 250 ng/mL Ca2+ionomycin for 16 hours, then stained with Mouse Anti-Human IL-17 Monoclonal Antibody (Catalog # MAB3171, dark orange filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-17/IL-17A

Interleukin-17 (IL-17) is a pro-inflammatory cytokine secreted by activated T cells. It is the prototype member of the IL-17 family that also includes IL-17B, C, D, E, and F.

Long Name
Interleukin 17
Entrez Gene IDs
3605 (Human); 16171 (Mouse); 301289 (Rat); 449530 (Porcine); 481837 (Canine); 102119976 (Cynomolgus Monkey)
Alternate Names
CTLA8; CTLA-8; CTLA8cytotoxic T-lymphocyte-associated serine esterase 8; Cytotoxic T-lymphocyte-associated antigen 8; IL17; IL-17; IL17A; IL-17A; IL-17Acytotoxic T-lymphocyte-associated protein 8; IL-17CTLA-8; IL17interleukin-17A; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17A

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Citations for Human IL-17/IL-17A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Establishment of a humanized mouse model of keloid diseases following the migration of patient immune cells to the lesion: Patient-derived keloid xenograft (PDKX) model
    Authors: Lee, AR;Lee, SY;Choi, JW;Um, IG;Na, HS;Lee, JH;Cho, ML;
    Experimental & molecular medicine
    Applications: IHC, Confocal Imaging
  2. Establishment of a humanized mouse model of keloid diseases following the migration of patient immune cells to the lesion: Patient-derived keloid xenograft (PDKX) model
    Authors: Lee, AR;Lee, SY;Choi, JW;Um, IG;Na, HS;Lee, JH;Cho, ML;
    Experimental & molecular medicine
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Impact of Adalimumab Treatment on Interleukin-17 and Interleukin-17 Receptor Expression in Skin and Synovium of Psoriatic Arthritis Patients with Mild Psoriasis
    Authors: JW Bolt, AW van Kuijk, MBM Teunissen, D van der Co, S Aarrass, DM Gerlag, PP Tak, MG van de San, MC Lebre, LGM van Baarse
    Biomedicines, 2022-01-29;10(2):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  4. Comparison of three different ELISAs for the detection of recombinant, native and plasma IL-17A
    Authors: MB Ismail, SO Åkefeldt, M Lourda, D Gavhed, R Gayet, M Aricò, JI Henter, C Delprat, H Valentin
    MethodsX, 2020-07-16;7(0):100997.
    Species: Human
    Sample Types: Plasma, Recombinant Protein
    Applications: ELISA Capture
  5. Patients with cystic fibrosis have inducible IL-17+IL-22+ memory cells in lung draining lymph nodes.
    Authors: Chan Y, Chen K, Duncan S, Lathrop K, Latoche J, Logar A, Pociask D, Wahlberg B, Ray P, Ray A, Pilewski J, Kolls J
    J Allergy Clin Immunol, 2012-07-11;131(4):1117-29, 1129.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  6. Langerhans cell histiocytosis reveals a new IL-17A-dependent pathway of dendritic cell fusion.
    Authors: Coury F, Annels N, Rivollier A, Olsson S, Santoro A, Speziani C, Azocar O, Flacher M, Djebali S, Tebib J, Brytting M, Egeler RM, Rabourdin-Combe C, Henter JI, Arico M, Delprat C
    Nat. Med., 2007-12-23;14(1):81-7.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P

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Reviews for Human IL-17/IL-17A Antibody

Average Rating: 4.5 (Based on 2 Reviews)

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Human IL-17/IL-17A Antibody
By Anonymous on 04/03/2023
Application: WB Sample Tested: Cell Lysates Species: Human

The Western blot of cell lysates obtained from human CD4+T cells. The well was loaded with 50 micrograms of sample under reducing conditions.


Human IL-17/IL-17A Antibody
By Anonymous on 10/14/2021
Application: IHC Sample Tested: Ischemic brain tissue Species: Human