Human IL-17/IL-17A Antibody

Catalog # Availability Size / Price Qty
AF-317-NA
AF-317-SP
Detection of Human IL‑17 by Western Blot.
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Product Details
Citations (41)
FAQs
Supplemental Products
Reviews (4)

Human IL-17/IL-17A Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-17 in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant canine IL-17 is observed, approximately 10% cross-reactivity with recombinant human IL-17F is observed and less than 5% cross-reactivity with recombinant mouse IL‑17 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human IL-17
Ile20-Ala155
Accession # Q16552
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
1-15 µg/mL
See below
Immunoprecipitation
3 µg/100 µg cell lysate
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
5-15 µg/mL
Immersion fixed human peripheral blood mononuclear cells treated with PHA
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
See below
Neutralization
Measured by its ability to neutralize IL‑17-induced IL‑6 secretion in NIH/3T3 mouse embryonic fibroblasts. Yao, Z. et al. (1995) Immunity 3:811. The Neutralization Dose (ND50) is typically 0.02-0.12 µg/mL in the presence of 15 ng/mL Recombinant Human IL‑17.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human IL‑17 by Western Blot. View Larger

Detection of Human IL‑17 by Western Blot.

Western blot shows lysates of human primary naïve CD4+ T cells, along with whole cell lysates (WCL) and conditioned-media supernatant (Supe) of human primary differentiated Th17 cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human
IL‑17 Antigen Affinity-purified Polyclonal Antibody
(Catalog # AF‑317‑NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL‑17 at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry IL‑17/IL‑17A  in Human Tonsil. View Larger

IL‑17/IL‑17A in Human Tonsil. IL‑17/IL‑17A was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human IL‑17/IL‑17A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-317-NA) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Immunoprecipitation Immunoprecipitation of Human IL‑17. View Larger

Immunoprecipitation of Human IL‑17.

Human IL-17 was immunoprecipitated from 100 μg of human primary differentiated Th17 cell lysate following incubation with 3 µg Goat Anti-Human IL-17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-317-NA) or control antibody (Catalog # AB-108-C) overnight at 4 °C. IL-17-antibody complexes were absorbed using Protein G Sepharose. Immunoprecipitated IL-17 was detected by Western blot using 1 µg/mL Goat Anti-Human IL‑17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-317-NA). View our recommended buffer recipes for immunoprecipitation.

Neutralization IL‑6 Secretion Induced by IL‑17 and Neutralization by Human IL‑17 Antibody. View Larger

IL‑6 Secretion Induced by IL‑17 and Neutralization by Human IL‑17 Antibody.

Recombinant Human IL‑17 (Catalog # 317-ILB) stimulates IL‑6 secretion in NIH/3T3 mouse embryonic fibroblasts in a dose-dependent manner (orange line), as measured by the mouse IL‑6 Quantikine ELISA Kit (Catalog # M6000B). IL‑6 secretion elicited by Recombinant Human IL‑17 (15 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‑17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-317-NA). The ND50 is typically
0.02‑0.12 µg/mL.

 

Intracellular Staining by Flow Cytometry Detection of IL‑17 in Human PBMCs by Flow Cytometry. View Larger

Detection of IL‑17 in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells were unstimulated (light orange filled histogram) or treated with 50 ng/mL PMA and 250 ng/mL Ca2+ ionomycin for 16 hours, then stained with Goat Anti-Human IL‑17 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF‑317‑NA, dark orange filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-17/IL-17A

Interleukin 17 (also known as CTLA-8) is a T cell-expressed pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of herpesvirus Saimiri. cDNA clones encoding IL-17 have been isolated from activated rat, mouse and human T cells. Human IL-17 cDNA encodes a 155 amino acid (aa) residue precursor protein with a 19 amino acid residue signal peptide that is cleaved to yield the 136 aa residue mature IL-17 containing one potential N-linked glycosylation site. Both recombinant and natural IL-17 have been shown to exist as disulfide linked homodimers. At the amino acid level, human IL-17 shows 72% and 63% sequence identity with herpesvirus and rat IL-17, respectively. An IL-17 specific mouse cell surface receptor (IL-17 R) has recently been cloned. While the expression of IL-17 mRNA is restricted to activated T cells, the expression of mIL-17 R mRNA has been detected in virtually all cells and tissues tested. IL-17 exhibits multiple biological activities on a variety of cells including the induction of IL-6 and IL-8 production in fibroblasts, the enhancement of surface expression of ICAM-1 in fibroblasts, activation of NF-kappa B and costimulation of T cell proliferation.

Long Name
Interleukin 17
Entrez Gene IDs
3605 (Human); 16171 (Mouse); 301289 (Rat); 481837 (Canine)
Alternate Names
CTLA-8; CTLA8cytotoxic T-lymphocyte-associated serine esterase 8; Cytotoxic T-lymphocyte-associated antigen 8; IL17; IL-17; IL17A; IL-17A; IL-17Acytotoxic T-lymphocyte-associated protein 8; IL-17CTLA-8; IL17interleukin-17A; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17A

Product Datasheets

Citations for Human IL-17/IL-17A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

41 Citations: Showing 1 - 10
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  1. Expression of IL-23/Th17-related cytokines in basal cell carcinoma and in the response to medical treatments
    Authors: C Pellegrini, A Orlandi, G Costanza, A Di Stefani, A Piccioni, A Di Cesare, A Chiricozzi, A Ferlosio, K Peris, MC Fargnoli
    PLoS ONE, 2017;12(8):e0183415.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC paraffin embedded
  2. Chronic hepatitis C liver microenvironment: role of the Th17/Treg interplay related to fibrogenesis
    Authors: DA Rios, P Valva, PC Casciato, S Frias, M Soledad Ca, MI Gaillard, L Bezrodnik, J Bandi, O Galdame, B Ameigeiras, D Krasniansk, C Brodersen, E Mullen, EN Matteo, MV Preciado
    Sci Rep, 2017;7(1):13283.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  3. Zoonotic intestinal helminths interact with the canine immune system by modulating T cell responses and preventing dendritic cell maturation
    Authors: J Junginger, K Raue, K Wolf, E Janecek, VM Stein, A Tipold, AR Günzel-Ape, C Strube, M Hewicker-T
    Sci Rep, 2017;7(1):10310.
    Species: Canine
    Sample Types: Whole Cells
    Applications: Flow
  4. IL-17A mediates inflammatory and tissue remodelling events in early human tendinopathy
    Authors: Neal L Millar
    Sci Rep, 2016;6(0):27149.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC - Not specified
  5. Functional expression of aryl hydrocarbon receptor on mast cells populating human endometriotic tissues
    Lab Invest, 2016;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded
  6. Peritoneal carcinomatosis of colorectal cancer is characterized by structural and functional reorganization of the tumor microenvironment inducing senescence and proliferation arrest in cancer cells
    Authors: CT Seebauer, S Brunner, G Glockzin, P Piso, P Ruemmele, HJ Schlitt, EK Geissler, S Fichtner-F, R Kesselring
    Oncoimmunology, 2016;5(12):e1242543.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  7. Atopy and Inhaled Corticosteroid Use Associate with Fewer IL-17+ Cells in Asthmatic Airways
    PLoS ONE, 2016;11(8):e0161433.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC - Paraffin embedded
  8. Association of Lung Inflammatory Cells with Small Airways Function and Exhaled Breath Markers in Smokers - Is There a Specific Role for Mast Cells?
    Authors: Nussbaumer-Ochsner Y, Stolk J, Ferraz da Silva L, van Schadewijk A, de Jeu R, Prins F, Mauad T, Rabe K, Hiemstra P
    PLoS ONE, 2015;10(6):e0129426.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC Not Specified
  9. RORgammat(+) hematopoietic cells are necessary for tumor cell proliferation during colitis-associated tumorigenesis in mice.
    Authors: Martin M, Kesselring R, Saidou B, Brunner S, Schiechl G, Mouris V, Wege A, Rummele P, Schlitt H, Geissler E, Fichtner-Feigl S
    Eur J Immunol, 2015;45(6):1667-79.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded
  10. Correlations between immune response and vascularization qRT-PCR gene expression clusters in squamous cervical cancer.
    Authors: Punt S, Houwing-Duistermaat J, Schulkens I, Thijssen V, Osse E, de Kroon C, Griffioen A, Fleuren G, Gorter A, Jordanova E
    Mol Cancer, 2015;14(0):71.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC Paraffin-embedded
  11. Th1 response and systemic treg deficiency in inclusion body myositis.
    Authors: Allenbach Y, Chaara W, Rosenzwajg M, Six A, Prevel N, Mingozzi F, Wanschitz J, Musset L, Charuel J, Eymard B, Salomon B, Duyckaerts C, Maisonobe T, Dubourg O, Herson S, Klatzmann D, Benveniste O
    PLoS ONE, 2014;9(3):e88788.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  12. Allergic contact dermatitis in psoriasis patients: typical, delayed, and non-interacting.
    Authors: Quaranta M, Eyerich S, Knapp B, Nasorri F, Scarponi C, Mattii M, Garzorz N, Harlfinger A, Jaeger T, Grosber M, Pennino D, Mempel M, Schnopp C, Theis F, Albanesi C, Cavani A, Schmidt-Weber C, Ring J, Eyerich K
    PLoS ONE, 2014;9(7):e101814.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded
  13. Intratumor IL-17-positive mast cells are the major source of the IL-17 that is predictive of survival in gastric cancer patients.
    Authors: Liu, Xiaosun, Jin, Hailong, Zhang, Geer, Lin, Xianke, Chen, Chao, Sun, Jianyi, Zhang, Yu, Zhang, Qing, Yu, Jiren
    PLoS ONE, 2014;9(9):e106834.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC Not Specified
  14. Sera from remitting and secondary progressive multiple sclerosis patients disrupt the blood-brain barrier.
    Authors: Shimizu F, Tasaki A, Sano Y, Ju M, Nishihara H, Oishi M, Koga M, Kawai M, Kanda T
    PLoS ONE, 2014;9(3):e92872.
    Species: Human
    Sample Types: Serum
    Applications: Neut
  15. Upregulation of IL-17A/F from human lung tissue explants with cigarette smoke exposure: implications for COPD.
    Authors: Chang Y, Al-Alwan L, Alshakfa S, Audusseau S, Mogas A, Chouiali F, Nair P, Baglole C, Hamid Q, Eidelman D
    Respir Res, 2014;15(1):145.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: WB
  16. Interleukin-17A promotes MUC5AC expression and goblet cell hyperplasia in nasal polyps via the Act1-mediated pathway.
    Authors: Xia, Wentong, Bai, Jing, Wu, Xingmei, Wei, Yi, Feng, Shaoyan, Li, Lei, Zhang, Jia, Xiong, Guanxia, Fan, Yunping, Shi, Jianbo, Li, Huabin
    PLoS ONE, 2014;9(6):e98915.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  17. In vivo induction of cutaneous inflammation results in the accumulation of extracellular trap-forming neutrophils expressing RORgammat and IL-17.
    Authors: Keijsers R, Hendriks A, van Erp P, van Cranenbroek B, van de Kerkhof P, Koenen H, Joosten I
    J Invest Dermatol, 2014;134(5):1276-84.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded
  18. The effect of adalimumab on key drivers in the pathogenesis of psoriasis.
    Authors: Hendriks A, van der Velden H, Wolberink E, Seyger M, Schalkwijk J, Zeeuwen P, de Jong E, Pasch M, van Erp P, van de Kerkhof P
    Br J Dermatol, 2014;170(3):571-80.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  19. IL-17/Th17 pathway is activated in acne lesions.
    Authors: Kelhala H, Palatsi R, Fyhrquist N, Lehtimaki S, Vayrynen J, Kallioinen M, Kubin M, Greco D, Tasanen K, Alenius H, Bertino B, Carlavan I, Mehul B, Deret S, Reiniche P, Martel P, Marty C, Blume-Peytavi U, Voegel J, Lauerma A
    PLoS ONE, 2014;9(8):e105238.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  20. IL-17A induces endothelial inflammation in systemic sclerosis via the ERK signaling pathway.
    Authors: Xing X, Yang J, Yang X, Wei Y, Zhu L, Gao D, Li M
    PLoS ONE, 2013;8(12):e85032.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  21. Reference range and short- and long-term biological variation of interleukin (IL)-6, IL-17A and tissue necrosis factor-alpha using high sensitivity assays.
    Authors: Todd J, Simpson P, Estis J, Torres V, Wub A
    Cytokine, 2013;64(3):660-5.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  22. Chemotherapy activates cancer-associated fibroblasts to maintain colorectal cancer-initiating cells by IL-17A.
    Authors: Lotti, Fiorenza, Jarrar, Awad M, Pai, Rish K, Hitomi, Masahiro, Lathia, Justin, Mace, Adam, Gantt, Gerald A, Sukhdeo, Kumar, DeVecchio, Jennifer, Vasanji, Amit, Leahy, Patrick, Hjelmeland, Anita B, Kalady, Matthew, Rich, Jeremy N
    J Exp Med, 2013;210(13):2851-72.
    Species: Human
    Sample Types: Whole Cells
    Applications: IF
  23. Interleukin-17A+ cell counts are increased in systemic sclerosis skin and their number is inversely correlated with the extent of skin involvement.
    Authors: Truchetet M, Brembilla N, Montanari E, Lonati P, Raschi E, Zeni S, Fontao L, Meroni P, Chizzolini C
    Arthritis Rheum, 2013;65(5):1347-56.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC - Paraffin embedded
  24. Neutralization of the IL-17 axis diminishes neutrophil invasion and protects from ischemic stroke.
    Authors: Gelderblom M, Weymar A, Bernreuther C, Velden J, Arunachalam P, Steinbach K, Orthey E, Arumugam T, Leypoldt F, Simova O, Thom V, Friese M, Prinz I, Holscher C, Glatzel M, Korn T, Gerloff C, Tolosa E, Magnus T
    Blood, 2012;120(18):3793-802.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded
  25. Differential pattern and prognostic significance of CD4+, FOXP3+ and IL-17+ tumor infiltrating lymphocytes in ductal and lobular breast cancers.
    Authors: Droeser R, Zlobec I, Kilic E, Guth U, Heberer M, Spagnoli G, Oertli D, Tapia C
    BMC Cancer, 2012;12(0):134.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  26. Induction of endothelial nitric oxide synthase expression by IL-17 in human vascular endothelial cells: implications for vascular remodeling in transplant vasculopathy.
    Authors: Liu AC, Lee M, McManus BM, Choy JC
    J. Immunol., 2012;188(3):1544-50.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  27. RASA1 maintains the lymphatic vasculature in a quiescent functional state in mice.
    Authors: Lapinski PE, Kwon S, Lubeck BA
    J. Clin. Invest., 2012;122(2):733-47.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  28. Interleukin-17 expression in neutrophils and Th17 cells in cutaneous T-cell lymphoma associated with neutrophilic infiltrate of the skin.
    Authors: Fontao L, Brembilla NC, Masouye I, Kaya G, Prins C, Dupin N, Saurat JH, Chizzolini C, Piguet V
    Br. J. Dermatol., 2012;166(3):687-9.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  29. Expression of the T helper 17-associated cytokines IL-17A and IL-17F in asthma and COPD.
    Authors: Doe C, Bafadhel M, Siddiqui S, Desai D, Mistry V, Rugman P, McCormick M, Woods J, May R, Sleeman MA, Anderson IK, Brightling CE
    Chest, 2011;138(5):1140-7.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  30. Influence of the Notch system in the therapeutic response of American tegumentary leishmaniasis.
    Authors: Rodrigues KM, Oliveira MP, Maretti-Mira AC, Oliveira-Neto MP, Mattos MS, Silva L, Soares DA, Dolci EL, Perico RA, Pirmez C
    Br. J. Dermatol., 2011;164(6):1228-34.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC Frozen
  31. IL-22, but not IL-17, dominant environment in cutaneous T-cell lymphoma.
    Authors: Miyagaki T, Sugaya M, Suga H, Kamata M, Ohmatsu H, Fujita H, Asano Y, Tada Y, Kadono T, Sato S
    Clin. Cancer Res., 2011;17(24):7529-38.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded
  32. Overrepresentation of IL-17A and IL-22 producing CD8 T cells in lesional skin suggests their involvement in the pathogenesis of psoriasis.
    Authors: Res PC, Piskin G, de Boer OJ, van der Loos CM, Teeling P, Bos JD, Teunissen MB
    PLoS ONE, 2010;5(11):e14108.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded
  33. IL-17 amplifies human contact hypersensitivity by licensing hapten nonspecific Th1 cells to kill autologous keratinocytes.
    Authors: Pennino D, Eyerich K, Scarponi C, Carbone T, Eyerich S, Nasorri F, Garcovich S, Traidl-Hoffmann C, Albanesi C, Cavani A
    J. Immunol., 2010;184(9):4880-8.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded
  34. IL-17 in atopic eczema: linking allergen-specific adaptive and microbial-triggered innate immune response.
    Authors: Eyerich K, Pennino D, Scarponi C, Foerster S, Nasorri F, Behrendt H, Ring J, Traidl-Hoffmann C, Albanesi C, Cavani A
    J. Allergy Clin. Immunol., 2009;123(1):59-66.e4.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  35. IL-17 induces monocyte migration in rheumatoid arthritis.
    Authors: Shahrara S, Pickens SR, Dorfleutner A, Pope RM
    J. Immunol., 2009;182(6):3884-91.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  36. Increased numbers of circulating polyfunctional Th17 memory cells in patients with seronegative spondylarthritides.
    Authors: Jandus C, Bioley G, Rivals JP, Dudler J, Speiser D, Romero P
    Arthritis Rheum., 2008;58(8):2307-17.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  37. Interleukin-17-producing T cells are enriched in the joints of children with arthritis, but have a reciprocal relationship to regulatory T cell numbers.
    Authors: Nistala K, Moncrieffe H, Newton KR, Varsani H, Hunter P, Wedderburn LR
    Arthritis Rheum., 2008;58(3):875-87.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  38. Role of mesothelial cell-derived granulocyte colony-stimulating factor in interleukin-17-induced neutrophil accumulation in the peritoneum.
    Authors: Witowski J, Ksiazek K, Warnecke C, Kuzlan M, Korybalska K, Tayama H, Wisniewska-Elnur J, Pawlaczyk K, Trominska J, Breborowicz A, Jorres A
    Kidney Int., 2007;71(6):514-25.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  39. IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis.
    Authors: Numasaki M, Watanabe M, Suzuki T, Takahashi H, Nakamura A, McAllister F, Hishinuma T, Goto J, Lotze MT, Kolls JK, Sasaki H
    J. Immunol., 2005;175(9):6177-89.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded
  40. Dendritic cells induce MUC1 expression and polarization on human T cells by an IL-7-dependent mechanism.
    Authors: Vasir B, Avigan D, Wu Z, Crawford K, Turnquist S, Ren J, Kufe D
    J. Immunol., 2005;174(4):2376-86.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  41. Plasma cell-like morphology of Th1-cytokine-producing cells associated with the loss of CD3 expression.
    Authors: Page G, Sattler A, Kersten S, Thiel A, Radbruch A, Miossec P
    Am. J. Pathol., 2004;164(2):409-17.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded

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Human IL-17/IL-17A Antibody
By Anonymous on 04/13/2018
Application: IHC Sample Tested: Adult brain Species: Human

Published in https://www.ncbi.nlm.nih.gov/pubmed/28169287
Used at 10ug/ml.
Briefly, frozen brain sections were fixed in 4% PFA (Fisher Scientific), followed by antigen retrieval using heating in acid citric buffer (Vector, Burlingame, CA, USA). Endogenous avidin-biotin was blocked for 15 min (Vector). Sections were incubated with 10% horse serum in PBS (Biosera, Boussens, France) and Fc Receptor Blocking Solution was added (Human TruStain FcX Biolegend,
London, UK). Primary antibody was added overnight at 4 °C and detected with donkey anti-goat-biotin (ab6578, Abcam), followed by streptavidin-alkaline phosphatase (SA-5100, Vector) and visualised with the Vector Blue Alkaline Phosphatase Substrate Kit III (Vector).


Human IL-17/IL-17A Antibody
By Anonymous on 03/23/2018
Application: B/N Sample Tested: 4T1 mouse breast cancer cell line,Breast tissue Species: mouse tumor-infiltrate lympocytes,Mouse

Human IL‑17/IL‑17A Antibody
By Anonymous on 04/21/2017

Antigen retrieval pH 6.0 (20 min)
Dilution-1:100, 1 h incubation.
Detection with ImmPRESS HRP Anti-Goat Ig (Peroxidase) Polymer (# MP-7405-15 ) and TSA-Cy3 (#SAT704A001EA)


Human IL-17/IL-17A Antibody
By Anonymous on 04/21/2017
Application: IHC Sample Tested: Spleen tissue,Colon tissue Species: Human

Staining is done in Leica Bond autostainer. Epitope retrieval at pH 6 (20 min), IL17/IL17A dilution 1:100, incubation time-1h. Detected with TSA-Cy3