Home / IL-17/IL-17A / Human IL-17/IL-17A Antibody

Human IL-17/IL-17A Antibody

Catalog #: AF-317-NA
53 Citations 5 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF-317-NA
AF-317-SP

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Detection of Human IL‑17 by Western Blot.
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Citations (53)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-17/IL-17A Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-17 in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant canine IL-17 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human IL-17
Ile20-Ala155
Accession # Q16552
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
1-15 µg/mL
See below
Immunoprecipitation
3 µg/100 µg cell lysate
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
5-15 µg/mL
Immersion fixed human peripheral blood mononuclear cells treated with PHA
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
See below
Neutralization
Measured by its ability to neutralize IL‑17-induced IL‑6 secretion in NIH/3T3 mouse embryonic fibroblasts. Yao, Z. et al. (1995) Immunity 3:811. The Neutralization Dose (ND50) is typically 0.02-0.12 µg/mL in the presence of 15 ng/mL Recombinant Human IL‑17.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human IL-17 antibody by Western Blot. View Larger

Detection of Human IL‑17 by Western Blot. Western blot shows lysates of human primary naïve CD4+ T cells, along with whole cell lysates (WCL) and conditioned-media supernatant (Supe) of human primary differentiated Th17 cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human IL-17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-317-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for IL-17 at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry IL-17/IL-17A antibody in Human Tonsil by Immunohistochemistry (IHC-P). View Larger

IL‑17/IL‑17A in Human Tonsil. IL-17/IL-17A was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human IL-17/IL-17A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-317-NA) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Immunoprecipitation Immunoprecipitation of Human IL‑17. View Larger

Immunoprecipitation of Human IL‑17. Human IL-17 was immunoprecipitated from 100 µg of human primary differentiated Th17 cell lysate following incubation with 3 µg Goat Anti-Human IL-17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-317-NA) or control antibody (AB-108-C) overnight at 4 °C. IL-17-antibody complexes were absorbed using Protein G Sepharose. Immunoprecipitated IL-17 was detected by Western blot using 1 µg/mL Goat Anti-Human IL-17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-317-NA). View our recommended buffer recipes for immunoprecipitation.

Intracellular Staining by Flow Cytometry Detection of IL-17 antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of IL‑17 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells were unstimulated (light orange filled histogram) or treated with 50 ng/mL PMA and 250 ng/mL Ca 2+ionomycin for 16 hours, then stained with Goat Anti-Human IL-17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-317-NA, dark orange filled histogram) or isotype control antibody (AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (F0108). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Neutralization IL-6 Secretion Induced by IL-17 and Neutralization by Human IL-17 Antibody. View Larger

IL‑6 Secretion Induced by IL‑17 and Neutralization by Human IL‑17 Antibody. Recombinant Human IL-17 (317-ILB) stimulates IL-6 secretion in NIH/3T3 mouse embryonic fibroblasts in a dose-dependent manner (orange line), as measured by the mouse IL-6 Quantikine ELISA Kit (Catalog # M6000B). IL-6 secretion elicited by Recombinant Human IL-17 (15 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-317-NA). The ND50 is typically 0.02‑0.12 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-17/IL-17A

Interleukin 17 (also known as CTLA-8) is a T cell-expressed pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of herpesvirus Saimiri. cDNA clones encoding IL-17 have been isolated from activated rat, mouse and human T cells. Human IL-17 cDNA encodes a 155 amino acid (aa) residue precursor protein with a 19 amino acid residue signal peptide that is cleaved to yield the 136 aa residue mature IL-17 containing one potential N-linked glycosylation site. Both recombinant and natural IL-17 have been shown to exist as disulfide linked homodimers. At the amino acid level, human IL-17 shows 72% and 63% sequence identity with herpesvirus and rat IL-17, respectively. An IL-17 specific mouse cell surface receptor (IL-17 R) has recently been cloned. While the expression of IL-17 mRNA is restricted to activated T cells, the expression of mIL-17 R mRNA has been detected in virtually all cells and tissues tested. IL-17 exhibits multiple biological activities on a variety of cells including the induction of IL-6 and IL-8 production in fibroblasts, the enhancement of surface expression of ICAM-1 in fibroblasts, activation of NF-kappa B and costimulation of T cell proliferation.

Long Name
Interleukin 17
Entrez Gene IDs
3605 (Human); 16171 (Mouse); 301289 (Rat); 449530 (Porcine); 481837 (Canine); 102119976 (Cynomolgus Monkey)
Alternate Names
CTLA8; CTLA-8; CTLA8cytotoxic T-lymphocyte-associated serine esterase 8; Cytotoxic T-lymphocyte-associated antigen 8; IL17; IL-17; IL17A; IL-17A; IL-17Acytotoxic T-lymphocyte-associated protein 8; IL-17CTLA-8; IL17interleukin-17A; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17A

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Citations for Human IL-17/IL-17A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

53 Citations: Showing 1 - 10
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  1. Spatial transcriptomics landscape of lesions from non-communicable inflammatory skin diseases
    Authors: A Schäbitz, C Hillig, M Mubarak, M Jargosch, A Farnoud, E Scala, N Kurzen, AC Pilz, N Bhalla, J Thomas, M Stahle, T Biedermann, CB Schmidt-We, F Theis, N Garzorz-St, K Eyerich, MP Menden, S Eyerich
    Nature Communications, 2022-12-13;13(1):7729.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  2. Single cell transcriptomic landscape of diabetic foot ulcers
    Authors: G Theocharid, BE Thomas, D Sarkar, HL Mumme, WJR Pilcher, B Dwivedi, T Sandoval-S, RF Sîrbulescu, A Kafanas, I Mezghani, P Wang, A Lobao, IS Vlachos, B Dash, HC Hsia, V Horsley, SS Bhasin, A Veves, M Bhasin
    Nature Communications, 2022-01-10;13(1):181.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  3. Reduced Interleukin-17-Expressing Cells in Cutaneous Melanoma
    Authors: A Tosi, L Nardinocch, ML Carbone, L Capriotti, E Pagani, S Mastroeni, C Fortes, F Scopelliti, C Cattani, F Passarelli, A Rosato, S D'Atri, CM Failla, A Cavani
    Biomedicines, 2021-12-16;9(12):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  4. Condomless receptive anal intercourse is associated with markers of mucosal inflammation in a cohort of men who have sex with men in Atlanta, Georgia
    Authors: CF Kelley, I Pollack, R Yacoub, Z Zhu, VE Van Doren, S Gumber, RR Amara, V Fedirko, CS Kraft, TJB de Man, YJ Hu, C Grimsley A, PS Sullivan, RM Bostick
    Journal of the International AIDS Society, 2021-12-01;24(12):e25859.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  5. ROR&gammat inhibitors block both IL-17 and IL-22 conferring a potential advantage over anti-IL-17 alone to treat severe asthma
    Authors: D Lamb, D De Sousa, K Quast, K Fundel-Cle, JS Erjefält, C Sandén, HJ Hoffmann, M Kästle, R Schmid, K Menden, D Delic
    Respiratory Research, 2021-05-22;22(1):158.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  6. Hepatic lymphocytes involved in the pathogenesis of pediatric and adult non-alcoholic fatty liver disease
    Authors: V Cairoli, E De Matteo, D Rios, C Lezama, M Galoppo, P Casciato, E Mullen, C Giadans, G Bertot, MV Preciado, P Valva
    Scientific Reports, 2021-03-04;11(1):5129.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  7. Ex vivo culture of lesional psoriasis skin for pharmacological testing
    Authors: ML Tiirikaine, A Woetmann, H Norsgaard, LF Santamaria, P Lovato
    J. Dermatol. Sci., 2019-12-27;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: Tissue Culture
  8. Paradoxical psoriasis induced by Tnf-alpha blockade shows immunological features typical of the early phase of psoriasis development
    Authors: L Fania, M Morelli, C Scarponi, L Mercurio, F Scopelliti, C Cattani, GL Scaglione, T Tonanzi, MA Pilla, G Pagnanelli, C Mazzanti, G Girolomoni, A Cavani, S Madonna, C Albanesi
    J Pathol Clin Res, 2019-10-29;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  9. Interleukin (IL)-13 and IL-17A contribute to neo-osteogenesis in chronic rhinosinusitis by inducing RUNX2
    Authors: R Khalmurato, HW Shin, DW Kim, JW Park
    EBioMedicine, 2019-07-19;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  10. Composition and Clinical Impact of the Immunologic Tumor Microenvironment in Oral Squamous Cell Carcinoma
    Authors: M Boxberg, L Leising, K Steiger, M Jesinghaus, A Alkhamas, M Mielke, N Pfarr, C Götz, KD Wolff, W Weichert, A Kolk
    J. Immunol., 2018-12-10;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  11. Chronic hepatitis C liver microenvironment: role of the Th17/Treg interplay related to fibrogenesis
    Authors: DA Rios, P Valva, PC Casciato, S Frias, M Soledad Ca, MI Gaillard, L Bezrodnik, J Bandi, O Galdame, B Ameigeiras, D Krasniansk, C Brodersen, E Mullen, EN Matteo, MV Preciado
    Sci Rep, 2017-10-16;7(1):13283.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  12. Zoonotic intestinal helminths interact with the canine immune system by modulating T cell responses and preventing dendritic cell maturation
    Authors: J Junginger, K Raue, K Wolf, E Janecek, VM Stein, A Tipold, AR Günzel-Ape, C Strube, M Hewicker-T
    Sci Rep, 2017-09-04;7(1):10310.
    Species: Canine
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. Expression of IL-23/Th17-related cytokines in basal cell carcinoma and in the response to medical treatments
    Authors: C Pellegrini, A Orlandi, G Costanza, A Di Stefani, A Piccioni, A Di Cesare, A Chiricozzi, A Ferlosio, K Peris, MC Fargnoli
    PLoS ONE, 2017-08-22;12(8):e0183415.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  14. Peritoneal carcinomatosis of colorectal cancer is characterized by structural and functional reorganization of the tumor microenvironment inducing senescence and proliferation arrest in cancer cells
    Authors: CT Seebauer, S Brunner, G Glockzin, P Piso, P Ruemmele, HJ Schlitt, EK Geissler, S Fichtner-F, R Kesselring
    Oncoimmunology, 2016-10-14;5(12):e1242543.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  15. Atopy and Inhaled Corticosteroid Use Associate with Fewer IL-17+ Cells in Asthmatic Airways
    PLoS ONE, 2016-08-23;11(8):e0161433.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  16. Functional expression of aryl hydrocarbon receptor on mast cells populating human endometriotic tissues
    Lab Invest, 2016-06-27;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  17. IL-17A mediates inflammatory and tissue remodelling events in early human tendinopathy
    Authors: Neal L Millar
    Sci Rep, 2016-06-06;6(0):27149.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  18. Association of Lung Inflammatory Cells with Small Airways Function and Exhaled Breath Markers in Smokers - Is There a Specific Role for Mast Cells?
    Authors: Nussbaumer-Ochsner Y, Stolk J, Ferraz da Silva L, van Schadewijk A, de Jeu R, Prins F, Mauad T, Rabe K, Hiemstra P
    PLoS ONE, 2015-06-12;10(6):e0129426.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  19. RORgammat(+) hematopoietic cells are necessary for tumor cell proliferation during colitis-associated tumorigenesis in mice.
    Authors: Martin M, Kesselring R, Saidou B, Brunner S, Schiechl G, Mouris V, Wege A, Rummele P, Schlitt H, Geissler E, Fichtner-Feigl S
    Eur J Immunol, 2015-05-15;45(6):1667-79.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  20. Correlations between immune response and vascularization qRT-PCR gene expression clusters in squamous cervical cancer.
    Authors: Punt S, Houwing-Duistermaat J, Schulkens I, Thijssen V, Osse E, de Kroon C, Griffioen A, Fleuren G, Gorter A, Jordanova E
    Mol Cancer, 2015-03-31;14(0):71.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC-P
  21. Preferential recruitment of Th17 cells to cervical cancer via CCR6-CCL20 pathway.
    Authors: Yu, Qing, Lou, Xiang-mi, He, Yan
    PLoS ONE, 2015-03-13;10(3):e0120855.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  22. Upregulation of IL-17A/F from human lung tissue explants with cigarette smoke exposure: implications for COPD.
    Authors: Chang Y, Al-Alwan L, Alshakfa S, Audusseau S, Mogas A, Chouiali F, Nair P, Baglole C, Hamid Q, Eidelman D
    Respir Res, 2014-11-27;15(1):145.
    Species: Human
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC-P, Western Blot
  23. Intratumor IL-17-positive mast cells are the major source of the IL-17 that is predictive of survival in gastric cancer patients.
    Authors: Liu, Xiaosun, Jin, Hailong, Zhang, Geer, Lin, Xianke, Chen, Chao, Sun, Jianyi, Zhang, Yu, Zhang, Qing, Yu, Jiren
    PLoS ONE, 2014-09-08;9(9):e106834.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  24. IL-17/Th17 pathway is activated in acne lesions.
    Authors: Kelhala H, Palatsi R, Fyhrquist N, Lehtimaki S, Vayrynen J, Kallioinen M, Kubin M, Greco D, Tasanen K, Alenius H, Bertino B, Carlavan I, Mehul B, Deret S, Reiniche P, Martel P, Marty C, Blume-Peytavi U, Voegel J, Lauerma A
    PLoS ONE, 2014-08-25;9(8):e105238.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  25. Allergic contact dermatitis in psoriasis patients: typical, delayed, and non-interacting.
    Authors: Quaranta M, Eyerich S, Knapp B, Nasorri F, Scarponi C, Mattii M, Garzorz N, Harlfinger A, Jaeger T, Grosber M, Pennino D, Mempel M, Schnopp C, Theis F, Albanesi C, Cavani A, Schmidt-Weber C, Ring J, Eyerich K
    PLoS ONE, 2014-07-24;9(7):e101814.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  26. Interleukin-17A promotes MUC5AC expression and goblet cell hyperplasia in nasal polyps via the Act1-mediated pathway.
    Authors: Xia, Wentong, Bai, Jing, Wu, Xingmei, Wei, Yi, Feng, Shaoyan, Li, Lei, Zhang, Jia, Xiong, Guanxia, Fan, Yunping, Shi, Jianbo, Li, Huabin
    PLoS ONE, 2014-06-03;9(6):e98915.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  27. Sera from remitting and secondary progressive multiple sclerosis patients disrupt the blood-brain barrier.
    Authors: Shimizu F, Tasaki A, Sano Y, Ju M, Nishihara H, Oishi M, Koga M, Kawai M, Kanda T
    PLoS ONE, 2014-03-31;9(3):e92872.
    Species: Human
    Sample Types: Serum
    Applications: Neutralization
  28. Th1 response and systemic treg deficiency in inclusion body myositis.
    Authors: Allenbach Y, Chaara W, Rosenzwajg M, Six A, Prevel N, Mingozzi F, Wanschitz J, Musset L, Charuel J, Eymard B, Salomon B, Duyckaerts C, Maisonobe T, Dubourg O, Herson S, Klatzmann D, Benveniste O
    PLoS ONE, 2014-03-04;9(3):e88788.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  29. The effect of adalimumab on key drivers in the pathogenesis of psoriasis.
    Authors: Hendriks A, van der Velden H, Wolberink E, Seyger M, Schalkwijk J, Zeeuwen P, de Jong E, Pasch M, van Erp P, van de Kerkhof P
    Br J Dermatol, 2014-03-01;170(3):571-80.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  30. IL-17A induces endothelial inflammation in systemic sclerosis via the ERK signaling pathway.
    Authors: Xing X, Yang J, Yang X, Wei Y, Zhu L, Gao D, Li M
    PLoS ONE, 2013-12-23;8(12):e85032.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: IHC-P, Neutralization
  31. Chemotherapy activates cancer-associated fibroblasts to maintain colorectal cancer-initiating cells by IL-17A.
    Authors: Lotti, Fiorenza, Jarrar, Awad M, Pai, Rish K, Hitomi, Masahiro, Lathia, Justin, Mace, Adam, Gantt, Gerald A, Sukhdeo, Kumar, DeVecchio, Jennifer, Vasanji, Amit, Leahy, Patrick, Hjelmeland, Anita B, Kalady, Matthew, Rich, Jeremy N
    J Exp Med, 2013-12-09;210(13):2851-72.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC, Neutralization
  32. In vivo induction of cutaneous inflammation results in the accumulation of extracellular trap-forming neutrophils expressing RORgammat and IL-17.
    Authors: Keijsers R, Hendriks A, van Erp P, van Cranenbroek B, van de Kerkhof P, Koenen H, Joosten I
    J Invest Dermatol, 2013-12-06;134(5):1276-84.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  33. Reference range and short- and long-term biological variation of interleukin (IL)-6, IL-17A and tissue necrosis factor-alpha using high sensitivity assays.
    Authors: Todd J, Simpson P, Estis J, Torres V, Wub A
    Cytokine, 2013-10-12;64(3):660-5.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  34. Interleukin-17A+ cell counts are increased in systemic sclerosis skin and their number is inversely correlated with the extent of skin involvement.
    Authors: Truchetet M, Brembilla N, Montanari E, Lonati P, Raschi E, Zeni S, Fontao L, Meroni P, Chizzolini C
    Arthritis Rheum, 2013-05-01;65(5):1347-56.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  35. Neutralization of the IL-17 axis diminishes neutrophil invasion and protects from ischemic stroke.
    Authors: Gelderblom M, Weymar A, Bernreuther C, Velden J, Arunachalam P, Steinbach K, Orthey E, Arumugam T, Leypoldt F, Simova O, Thom V, Friese M, Prinz I, Holscher C, Glatzel M, Korn T, Gerloff C, Tolosa E, Magnus T
    Blood, 2012-09-13;120(18):3793-802.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  36. Differential pattern and prognostic significance of CD4+, FOXP3+ and IL-17+ tumor infiltrating lymphocytes in ductal and lobular breast cancers.
    Authors: Droeser R, Zlobec I, Kilic E, Guth U, Heberer M, Spagnoli G, Oertli D, Tapia C
    BMC Cancer, 2012-04-03;12(0):134.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  37. RASA1 maintains the lymphatic vasculature in a quiescent functional state in mice.
    Authors: Lapinski PE, Kwon S, Lubeck BA
    J. Clin. Invest., 2012-01-09;122(2):733-47.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: IHC-P, Neutralization
  38. Interleukin-17 expression in neutrophils and Th17 cells in cutaneous T-cell lymphoma associated with neutrophilic infiltrate of the skin.
    Authors: Fontao L, Brembilla NC, Masouye I, Kaya G, Prins C, Dupin N, Saurat JH, Chizzolini C, Piguet V
    Br. J. Dermatol., 2012-01-09;166(3):687-9.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  39. Induction of endothelial nitric oxide synthase expression by IL-17 in human vascular endothelial cells: implications for vascular remodeling in transplant vasculopathy.
    Authors: Liu AC, Lee M, McManus BM, Choy JC
    J. Immunol., 2012-01-04;188(3):1544-50.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: IHC, Neutralization
  40. IL-22, but not IL-17, dominant environment in cutaneous T-cell lymphoma.
    Authors: Miyagaki T, Sugaya M, Suga H, Kamata M, Ohmatsu H, Fujita H, Asano Y, Tada Y, Kadono T, Sato S
    Clin. Cancer Res., 2011-11-02;17(24):7529-38.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  41. Influence of the Notch system in the therapeutic response of American tegumentary leishmaniasis.
    Authors: Rodrigues KM, Oliveira MP, Maretti-Mira AC, Oliveira-Neto MP, Mattos MS, Silva L, Soares DA, Dolci EL, Perico RA, Pirmez C
    Br. J. Dermatol., 2011-05-17;164(6):1228-34.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  42. Delineation of a cellular hierarchy in lung cancer reveals an oncofetal antigen expressed on tumor-initiating cells.
    Authors: Damelin M, Geles KG, Follettie MT, Yuan P, Baxter M, Golas J, Dijoseph JF, Karnoub M, Huang S, Diesl V, Behrens C, Choe SE, Rios C, Gruzas J, Sridharan L, Dougher M, Kunz A, Hamann PR, Evans D, Armellino D, Khandke K, Marquette K, Tchistiakova L, Boghaert ER, Abraham RT, Wistuba II, Zhou BB
    Cancer Res., 2011-05-03;71(12):4236-46.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  43. Overrepresentation of IL-17A and IL-22 producing CD8 T cells in lesional skin suggests their involvement in the pathogenesis of psoriasis.
    Authors: Res PC, Piskin G, de Boer OJ, van der Loos CM, Teeling P, Bos JD, Teunissen MB
    PLoS ONE, 2010-11-24;5(11):e14108.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  44. Expression of the T helper 17-associated cytokines IL-17A and IL-17F in asthma and COPD.
    Authors: Doe C, Bafadhel M, Siddiqui S, Desai D, Mistry V, Rugman P, McCormick M, Woods J, May R, Sleeman MA, Anderson IK, Brightling CE
    Chest, 2010-06-10;138(5):1140-7.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  45. IL-17 amplifies human contact hypersensitivity by licensing hapten nonspecific Th1 cells to kill autologous keratinocytes.
    Authors: Pennino D, Eyerich K, Scarponi C, Carbone T, Eyerich S, Nasorri F, Garcovich S, Traidl-Hoffmann C, Albanesi C, Cavani A
    J. Immunol., 2010-03-31;184(9):4880-8.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  46. IL-17 induces monocyte migration in rheumatoid arthritis.
    Authors: Shahrara S, Pickens SR, Dorfleutner A, Pope RM
    J. Immunol., 2009-03-15;182(6):3884-91.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  47. IL-17 in atopic eczema: linking allergen-specific adaptive and microbial-triggered innate immune response.
    Authors: Eyerich K, Pennino D, Scarponi C, Foerster S, Nasorri F, Behrendt H, Ring J, Traidl-Hoffmann C, Albanesi C, Cavani A
    J. Allergy Clin. Immunol., 2008-12-03;123(1):59-66.e4.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  48. Increased numbers of circulating polyfunctional Th17 memory cells in patients with seronegative spondylarthritides.
    Authors: Jandus C, Bioley G, Rivals JP, Dudler J, Speiser D, Romero P
    Arthritis Rheum., 2008-08-01;58(8):2307-17.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC-P
  49. Interleukin-17-producing T cells are enriched in the joints of children with arthritis, but have a reciprocal relationship to regulatory T cell numbers.
    Authors: Nistala K, Moncrieffe H, Newton KR, Varsani H, Hunter P, Wedderburn LR
    Arthritis Rheum., 2008-03-01;58(3):875-87.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  50. Role of mesothelial cell-derived granulocyte colony-stimulating factor in interleukin-17-induced neutrophil accumulation in the peritoneum.
    Authors: Witowski J, Ksiazek K, Warnecke C, Kuzlan M, Korybalska K, Tayama H, Wisniewska-Elnur J, Pawlaczyk K, Trominska J, Breborowicz A, Jorres A
    Kidney Int., 2007-01-17;71(6):514-25.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  51. IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis.
    Authors: Numasaki M, Watanabe M, Suzuki T, Takahashi H, Nakamura A, McAllister F, Hishinuma T, Goto J, Lotze MT, Kolls JK, Sasaki H
    J. Immunol., 2005-11-01;175(9):6177-89.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  52. Dendritic cells induce MUC1 expression and polarization on human T cells by an IL-7-dependent mechanism.
    Authors: Vasir B, Avigan D, Wu Z, Crawford K, Turnquist S, Ren J, Kufe D
    J. Immunol., 2005-02-15;174(4):2376-86.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  53. Plasma cell-like morphology of Th1-cytokine-producing cells associated with the loss of CD3 expression.
    Authors: Page G, Sattler A, Kersten S, Thiel A, Radbruch A, Miossec P
    Am. J. Pathol., 2004-02-01;164(2):409-17.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P

FAQs

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Isotype Controls

Normal Goat IgG Control

AB-108-C
377 Citations
20 Reviews
1 Image
Ctrl

Reconstitution Buffers

Reconstitution Buffer 1 (PBS)

RB01
3 Reviews

Secondary Antibodies

Chicken Anti-Goat IgG Biotinylated Antibody

BAF019
WB

Donkey Anti-Goat IgG Antibody

AF109
8 Citations
WB

Donkey Anti-Goat IgG Biotinylated Antibody

BAF109
3 Citations
1 Review
WB

Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

NL003
8 Citations
Flow,IHC,ICC

Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

NL001
28 Citations
2 Reviews
Flow,IHC,ICC

Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

NL002
5 Citations
Flow,IHC,ICC

Goat IgG (H+L) APC-conjugated Antibody

F0108
14 Citations
1 Image
Flow

Goat IgG (H+L) Antibody

R-401-C-ABS
WB,ELISA

Goat IgG (H+L) Fluorescein-conjugated Antibody

F0109
3 Citations
1 Image
Flow

Goat IgG (H+L) PE-conjugated Antibody

F0107
11 Citations
2 Reviews
1 Image
Flow

Goat IgG (H+L) PerCP-conjugated Antibody

F0124
Flow

Goat IgG HRP-conjugated Antibody

HAF017
58 Citations
10 Reviews
1 Image
WB

Goat IgG HRP-conjugated Antibody

HAF109
51 Citations
4 Reviews
2 Images
WB,Simple Western

Goat IgG Horseradish Peroxidase-conjugated Antibody

HAF019
21 Citations
2 Images
WB,IHC

Goat IgG VisUCyte HRP Polymer

VC004
8 Citations
1 Image
IHC

Rabbit Anti-Goat IgG Biotinylated Antibody

BAF017
2 Citations
1 Review
WB

Reviews for Human IL-17/IL-17A Antibody

Average Rating: 4.2 (Based on 5 Reviews)

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Human IL-17/IL-17A Antibody
By Anonymous on 09/13/2023
Application: IHC Sample Tested: Tonsil tissue Species: Human

FFPE heating antigen retrieval in pH9
primary Ab x100 in 1% BSA (IgG free) for 1 hour
biotinylated rabbit anti-goat and streptavidin-HRP + DAB

Immunohistochemistry Human IL-17/IL-17A Antibody AF-317-NA
Human IL-17/IL-17A Antibody
By Stephanie Zandee on 04/13/2018
Application: IHC Sample Tested: Adult brain Species: Human

Published in https://www.ncbi.nlm.nih.gov/pubmed/28169287
Used at 10ug/ml.
Briefly, frozen brain sections were fixed in 4% PFA (Fisher Scientific), followed by antigen retrieval using heating in acid citric buffer (Vector, Burlingame, CA, USA). Endogenous avidin-biotin was blocked for 15 min (Vector). Sections were incubated with 10% horse serum in PBS (Biosera, Boussens, France) and Fc Receptor Blocking Solution was added (Human TruStain FcX Biolegend,
London, UK). Primary antibody was added overnight at 4 °C and detected with donkey anti-goat-biotin (ab6578, Abcam), followed by streptavidin-alkaline phosphatase (SA-5100, Vector) and visualised with the Vector Blue Alkaline Phosphatase Substrate Kit III (Vector).

Immunohistochemistry Human IL-17/IL-17A Antibody AF-317-NA
Human IL-17/IL-17A Antibody
By BAREUN KIM on 03/23/2018
Application: B/N Sample Tested: 4T1 mouse breast cancer cell line,Breast tissue Species: Mouse and mouse tumor-infiltrate lympocytes
By Nana Feinberg on 04/21/2017

Antigen retrieval pH 6.0 (20 min)
Dilution-1:100, 1 h incubation.
Detection with ImmPRESS HRP Anti-Goat Ig (Peroxidase) Polymer (# MP-7405-15 ) and TSA-Cy3 (#SAT704A001EA)

AF-317-NA
Human IL-17/IL-17A Antibody
By Nana Feinberg on 04/21/2017
Application: IHC Sample Tested: Colon tissue,Spleen tissue Species: Human

Staining is done in Leica Bond autostainer. Epitope retrieval at pH 6 (20 min), IL17/IL17A dilution 1:100, incubation time-1h. Detected with TSA-Cy3

Immunohistochemistry Human IL-17/IL-17A Antibody AF-317-NA