Human IL-17/IL-17A Antibody

(38 citations)
(2 Reviews)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human IL-17 in direct ELISAs and Western blots. In direct ELISAs, approximately 60% cross-reactivity with recombinant canine IL-17 is observed, approximately 10% cross-reactivity with recombinant human (rh) IL-17F and with recombinant mouse IL‑17 is observed, and less than 1% cross-reactivity with rhIL-17B, rhIL-17C, rhIL-17D, and rhIL‑17E is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human IL-17
    Ile20-Ala155
    Accession # Q16552
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunohistochemistry
    1-15 µg/mL
    See below
  • Immunoprecipitation
    3 µg/100 µg cell lysate
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    5-15 µg/mL
    Immersion fixed human peripheral blood mononuclear cells treated with PHA
  • Intracellular Staining by Flow Cytometry
    2.5 µg/106 cells
    See below
  • Neutralization

    Measured by its ability to neutralize IL‑17-induced IL‑6 secretion in NIH/3T3 mouse embryonic fibroblasts. Yao, Z. et al. (1995) Immunity 3:811. The Neutralization Dose (ND50) is typically 0.02-0.12 µg/mL in the presence of 15 ng/mL Recombinant Human IL‑17.

     

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human IL‑17 by Western Blot.

Western blot shows lysates of human primary naïve CD4+ T cells, along with whole cell lysates (WCL) and conditioned-media supernatant (Supe) of human primary differentiated Th17 cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human
IL‑17 Antigen Affinity-purified Polyclonal Antibody
(Catalog # AF‑317‑NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL‑17 at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry
IL‑17/IL‑17A in Human Tonsil. IL‑17/IL‑17A was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human IL‑17/IL‑17A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-317-NA) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Immunoprecipitation
Immunoprecipitation of Human IL‑17.

Human IL-17 was immunoprecipitated from 100 μg of human primary differentiated Th17 cell lysate following incubation with 3 µg Goat Anti-Human IL-17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-317-NA) or control antibody (Catalog # AB-108-C) overnight at 4 °C. IL-17-antibody complexes were absorbed using Protein G Sepharose. Immunoprecipitated IL-17 was detected by Western blot using 1 µg/mL Goat Anti-Human IL-17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-317-NA). View our recommended buffer recipes for immunoprecipitation.

IL‑6 Secretion Induced by IL‑17 and Neutralization by Human IL‑17 Antibody.

Recombinant Human IL‑17 (Catalog # 317-ILB) stimulates IL‑6 secretion in NIH/3T3 mouse embryonic fibroblasts in a dose-dependent manner (orange line), as measured by the mouse IL‑6 Quantikine ELISA Kit (Catalog # M6000B). IL‑6 secretion elicited by Recombinant Human IL‑17 (15 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‑17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-317-NA). The ND50 is typically
0.02‑0.12 µg/mL.

 

Intracellular Staining by Flow Cytometry
Detection of IL‑17 in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells were unstimulated (light orange filled histogram) or treated with 50 ng/mL PMA and 250 ng/mL Ca2+ ionomycin for 16 hours, then stained with Goat Anti-Human IL‑17 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF‑317‑NA, dark orange filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-17/IL-17A

Interleukin 17 (also known as CTLA-8) is a T cell-expressed pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of herpesvirus Saimiri. cDNA clones encoding IL-17 have been isolated from activated rat, mouse and human T cells. Human IL-17 cDNA encodes a 155 amino acid (aa) residue precursor protein with a 19 amino acid residue signal peptide that is cleaved to yield the 136 aa residue mature IL-17 containing one potential N-linked glycosylation site. Both recombinant and natural IL-17 have been shown to exist as disulfide linked homodimers. At the amino acid level, human IL-17 shows 72% and 63% sequence identity with herpesvirus and rat IL-17, respectively. An IL-17 specific mouse cell surface receptor (IL-17 R) has recently been cloned. While the expression of IL-17 mRNA is restricted to activated T cells, the expression of mIL-17 R mRNA has been detected in virtually all cells and tissues tested. IL-17 exhibits multiple biological activities on a variety of cells including the induction of IL-6 and IL-8 production in fibroblasts, the enhancement of surface expression of ICAM-1 in fibroblasts, activation of NF-kappa B and costimulation of T cell proliferation.

  • Long Name:
    Interleukin 17
  • Entrez Gene IDs:
    3605 (Human); 16171 (Mouse); 301289 (Rat); 481837 (Canine)
  • Alternate Names:
    CTLA-8; CTLA8cytotoxic T-lymphocyte-associated serine esterase 8; Cytotoxic T-lymphocyte-associated antigen 8; IL17; IL-17; IL17A; IL-17A; IL-17Acytotoxic T-lymphocyte-associated protein 8; IL-17CTLA-8; IL17interleukin-17A; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17A
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

38 Citations: Showing 1 - 10
Filter your results:

Species
Applications
Sample Type
  1. Expression of IL-23/Th17-related cytokines in basal cell carcinoma and in the response to medical treatments
    Authors: C Pellegrini, A Orlandi, G Costanza, A Di Stefani, A Piccioni, A Di Cesare, A Chiricozzi, A Ferlosio, K Peris, MC Fargnoli
    PLoS ONE, 2017;12(8):e0183415.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC paraffin embedded
  2. IL-17A mediates inflammatory and tissue remodelling events in early human tendinopathy
    Authors: Neal L Millar
    Sci Rep, 2016;6(0):27149.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC - Not specified
  3. Functional expression of aryl hydrocarbon receptor on mast cells populating human endometriotic tissues
    Lab Invest, 2016;0(0):.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  4. Atopy and Inhaled Corticosteroid Use Associate with Fewer IL-17+ Cells in Asthmatic Airways
    PLoS ONE, 2016;11(8):e0161433.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC - Paraffin embedded
  5. Association of Lung Inflammatory Cells with Small Airways Function and Exhaled Breath Markers in Smokers - Is There a Specific Role for Mast Cells?
    Authors: Nussbaumer-Ochsner Y, Stolk J, Ferraz da Silva L, van Schadewijk A, de Jeu R, Prins F, Mauad T, Rabe K, Hiemstra P
    PLoS ONE, 2015;10(6):e0129426.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Not Specified
  6. RORgammat(+) hematopoietic cells are necessary for tumor cell proliferation during colitis-associated tumorigenesis in mice.
    Authors: Martin M, Kesselring R, Saidou B, Brunner S, Schiechl G, Mouris V, Wege A, Rummele P, Schlitt H, Geissler E, Fichtner-Feigl S
    Eur J Immunol, 2015;45(6):1667-79.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  7. Correlations between immune response and vascularization qRT-PCR gene expression clusters in squamous cervical cancer.
    Authors: Punt S, Houwing-Duistermaat J, Schulkens I, Thijssen V, Osse E, de Kroon C, Griffioen A, Fleuren G, Gorter A, Jordanova E
    Mol Cancer, 2015;14(0):71.
    Species: Human
    Sample Type: Whole Cells
    Application: IHC Paraffin-embedded
  8. The effect of adalimumab on key drivers in the pathogenesis of psoriasis.
    Authors: Hendriks A, van der Velden H, Wolberink E, Seyger M, Schalkwijk J, Zeeuwen P, de Jong E, Pasch M, van Erp P, van de Kerkhof P
    Br J Dermatol, 2014;170(3):571-80.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  9. In vivo induction of cutaneous inflammation results in the accumulation of extracellular trap-forming neutrophils expressing RORgammat and IL-17.
    Authors: Keijsers R, Hendriks A, van Erp P, van Cranenbroek B, van de Kerkhof P, Koenen H, Joosten I
    J Invest Dermatol, 2014;134(5):1276-84.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  10. Intratumor IL-17-positive mast cells are the major source of the IL-17 that is predictive of survival in gastric cancer patients.
    Authors: Liu, Xiaosun, Jin, Hailong, Zhang, Geer, Lin, Xianke, Chen, Chao, Sun, Jianyi, Zhang, Yu, Zhang, Qing, Yu, Jiren
    PLoS ONE, 2014;9(9):e106834.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Not Specified
  11. Sera from remitting and secondary progressive multiple sclerosis patients disrupt the blood-brain barrier.
    Authors: Shimizu F, Tasaki A, Sano Y, Ju M, Nishihara H, Oishi M, Koga M, Kawai M, Kanda T
    PLoS ONE, 2014;9(3):e92872.
    Species: Human
    Sample Type: Serum
    Application: Neut
  12. Allergic contact dermatitis in psoriasis patients: typical, delayed, and non-interacting.
    Authors: Quaranta M, Eyerich S, Knapp B, Nasorri F, Scarponi C, Mattii M, Garzorz N, Harlfinger A, Jaeger T, Grosber M, Pennino D, Mempel M, Schnopp C, Theis F, Albanesi C, Cavani A, Schmidt-Weber C, Ring J, Eyerich K
    PLoS ONE, 2014;9(7):e101814.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  13. Upregulation of IL-17A/F from human lung tissue explants with cigarette smoke exposure: implications for COPD.
    Authors: Chang Y, Al-Alwan L, Alshakfa S, Audusseau S, Mogas A, Chouiali F, Nair P, Baglole C, Hamid Q, Eidelman D
    Respir Res, 2014;15(1):145.
    Species: Human
    Sample Type: Tissue Homogenates
    Application: WB
  14. Th1 response and systemic treg deficiency in inclusion body myositis.
    Authors: Allenbach Y, Chaara W, Rosenzwajg M, Six A, Prevel N, Mingozzi F, Wanschitz J, Musset L, Charuel J, Eymard B, Salomon B, Duyckaerts C, Maisonobe T, Dubourg O, Herson S, Klatzmann D, Benveniste O
    PLoS ONE, 2014;9(3):e88788.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  15. IL-17/Th17 pathway is activated in acne lesions.
    Authors: Kelhala H, Palatsi R, Fyhrquist N, Lehtimaki S, Vayrynen J, Kallioinen M, Kubin M, Greco D, Tasanen K, Alenius H, Bertino B, Carlavan I, Mehul B, Deret S, Reiniche P, Martel P, Marty C, Blume-Peytavi U, Voegel J, Lauerma A
    PLoS ONE, 2014;9(8):e105238.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  16. Interleukin-17A promotes MUC5AC expression and goblet cell hyperplasia in nasal polyps via the Act1-mediated pathway.
    Authors: Xia, Wentong, Bai, Jing, Wu, Xingmei, Wei, Yi, Feng, Shaoyan, Li, Lei, Zhang, Jia, Xiong, Guanxia, Fan, Yunping, Shi, Jianbo, Li, Huabin
    PLoS ONE, 2014;9(6):e98915.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  17. Interleukin-17A+ cell counts are increased in systemic sclerosis skin and their number is inversely correlated with the extent of skin involvement.
    Authors: Truchetet M, Brembilla N, Montanari E, Lonati P, Raschi E, Zeni S, Fontao L, Meroni P, Chizzolini C
    Arthritis Rheum, 2013;65(5):1347-56.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC - Paraffin embedded
  18. Reference range and short- and long-term biological variation of interleukin (IL)-6, IL-17A and tissue necrosis factor-alpha using high sensitivity assays.
    Authors: Todd J, Simpson P, Estis J, Torres V, Wub A
    Cytokine, 2013;64(3):660-5.
    Species: Human
    Sample Type: Plasma
    Application: ELISA Development
  19. IL-17A induces endothelial inflammation in systemic sclerosis via the ERK signaling pathway.
    Authors: Xing X, Yang J, Yang X, Wei Y, Zhu L, Gao D, Li M
    PLoS ONE, 2013;8(12):e85032.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  20. Chemotherapy activates cancer-associated fibroblasts to maintain colorectal cancer-initiating cells by IL-17A.
    Authors: Lotti, Fiorenza, Jarrar, Awad M, Pai, Rish K, Hitomi, Masahiro, Lathia, Justin, Mace, Adam, Gantt, Gerald A, Sukhdeo, Kumar, DeVecchio, Jennifer, Vasanji, Amit, Leahy, Patrick, Hjelmeland, Anita B, Kalady, Matthew, Rich, Jeremy N
    J Exp Med, 2013;210(13):2851-72.
    Species: Human
    Sample Type: Whole Cells
    Application: IF
  21. Differential pattern and prognostic significance of CD4+, FOXP3+ and IL-17+ tumor infiltrating lymphocytes in ductal and lobular breast cancers.
    Authors: Droeser R, Zlobec I, Kilic E, Guth U, Heberer M, Spagnoli G, Oertli D, Tapia C
    BMC Cancer, 2012;12(0):134.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  22. Interleukin-17 expression in neutrophils and Th17 cells in cutaneous T-cell lymphoma associated with neutrophilic infiltrate of the skin.
    Authors: Fontao L, Brembilla NC, Masouye I, Kaya G, Prins C, Dupin N, Saurat JH, Chizzolini C, Piguet V
    Br. J. Dermatol., 2012;166(3):687-9.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  23. Neutralization of the IL-17 axis diminishes neutrophil invasion and protects from ischemic stroke.
    Authors: Gelderblom M, Weymar A, Bernreuther C, Velden J, Arunachalam P, Steinbach K, Orthey E, Arumugam T, Leypoldt F, Simova O, Thom V, Friese M, Prinz I, Holscher C, Glatzel M, Korn T, Gerloff C, Tolosa E, Magnus T
    Blood, 2012;120(18):3793-802.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  24. RASA1 maintains the lymphatic vasculature in a quiescent functional state in mice.
    Authors: Lapinski PE, Kwon S, Lubeck BA
    J. Clin. Invest., 2012;122(2):733-47.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  25. Induction of endothelial nitric oxide synthase expression by IL-17 in human vascular endothelial cells: implications for vascular remodeling in transplant vasculopathy.
    Authors: Liu AC, Lee M, McManus BM, Choy JC
    J. Immunol., 2012;188(3):1544-50.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  26. Expression of the T helper 17-associated cytokines IL-17A and IL-17F in asthma and COPD.
    Authors: Doe C, Bafadhel M, Siddiqui S, Desai D, Mistry V, Rugman P, McCormick M, Woods J, May R, Sleeman MA, Anderson IK, Brightling CE
    Chest, 2011;138(5):1140-7.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  27. Influence of the Notch system in the therapeutic response of American tegumentary leishmaniasis.
    Authors: Rodrigues KM, Oliveira MP, Maretti-Mira AC, Oliveira-Neto MP, Mattos MS, Silva L, Soares DA, Dolci EL, Perico RA, Pirmez C
    Br. J. Dermatol., 2011;164(6):1228-34.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Frozen
  28. IL-22, but not IL-17, dominant environment in cutaneous T-cell lymphoma.
    Authors: Miyagaki T, Sugaya M, Suga H, Kamata M, Ohmatsu H, Fujita H, Asano Y, Tada Y, Kadono T, Sato S
    Clin. Cancer Res., 2011;17(24):7529-38.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  29. IL-17 amplifies human contact hypersensitivity by licensing hapten nonspecific Th1 cells to kill autologous keratinocytes.
    Authors: Pennino D, Eyerich K, Scarponi C, Carbone T, Eyerich S, Nasorri F, Garcovich S, Traidl-Hoffmann C, Albanesi C, Cavani A
    J. Immunol., 2010;184(9):4880-8.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  30. Overrepresentation of IL-17A and IL-22 producing CD8 T cells in lesional skin suggests their involvement in the pathogenesis of psoriasis.
    Authors: Res PC, Piskin G, de Boer OJ, van der Loos CM, Teeling P, Bos JD, Teunissen MB
    PLoS ONE, 2010;5(11):e14108.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  31. IL-17 in atopic eczema: linking allergen-specific adaptive and microbial-triggered innate immune response.
    Authors: Eyerich K, Pennino D, Scarponi C, Foerster S, Nasorri F, Behrendt H, Ring J, Traidl-Hoffmann C, Albanesi C, Cavani A
    J. Allergy Clin. Immunol., 2009;123(1):59-66.e4.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  32. IL-17 induces monocyte migration in rheumatoid arthritis.
    Authors: Shahrara S, Pickens SR, Dorfleutner A, Pope RM
    J. Immunol., 2009;182(6):3884-91.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  33. Increased numbers of circulating polyfunctional Th17 memory cells in patients with seronegative spondylarthritides.
    Authors: Jandus C, Bioley G, Rivals JP, Dudler J, Speiser D, Romero P
    Arthritis Rheum., 2008;58(8):2307-17.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  34. Interleukin-17-producing T cells are enriched in the joints of children with arthritis, but have a reciprocal relationship to regulatory T cell numbers.
    Authors: Nistala K, Moncrieffe H, Newton KR, Varsani H, Hunter P, Wedderburn LR
    Arthritis Rheum., 2008;58(3):875-87.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  35. Role of mesothelial cell-derived granulocyte colony-stimulating factor in interleukin-17-induced neutrophil accumulation in the peritoneum.
    Authors: Witowski J, Ksiazek K, Warnecke C, Kuzlan M, Korybalska K, Tayama H, Wisniewska-Elnur J, Pawlaczyk K, Trominska J, Breborowicz A, Jorres A
    Kidney Int., 2007;71(6):514-25.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  36. IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis.
    Authors: Numasaki M, Watanabe M, Suzuki T, Takahashi H, Nakamura A, McAllister F, Hishinuma T, Goto J, Lotze MT, Kolls JK, Sasaki H
    J. Immunol., 2005;175(9):6177-89.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  37. Dendritic cells induce MUC1 expression and polarization on human T cells by an IL-7-dependent mechanism.
    Authors: Vasir B, Avigan D, Wu Z, Crawford K, Turnquist S, Ren J, Kufe D
    J. Immunol., 2005;174(4):2376-86.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  38. Plasma cell-like morphology of Th1-cytokine-producing cells associated with the loss of CD3 expression.
    Authors: Page G, Sattler A, Kersten S, Thiel A, Radbruch A, Miossec P
    Am. J. Pathol., 2004;164(2):409-17.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
Expand to show all 38 Citations
Cell and Tissue Staining Kits
Description Application Cat# Citations Images  

Anti-Goat HRP-DAB Cell & Tissue Staining Kit

CTS008 13
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
ExactaChIP Chromatin Immunoprecipitation Kits
Description Application Cat# Citations Images  

ExactaChIP Chromatin IP Buffer Panel

ChIP ECP001 2
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Immunohistochemistry Reagents
Description Application Cat# Citations Images  

Antigen Retrieval Reagent-Universal

CTS015 10
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Antigen Retrieval Reagent-Basic

CTS013 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mounting Medium

CTS011 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Antigen Retrieval Reagent Sampler (50 mL each)

CTS016
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Antigen Retrieval Reagent-Acidic

CTS014
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

NorthernLights Guard Mounting Media

NL996
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

VisUCyte Antigen Retrieval Reagent-Universal

VCTS023
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

VisUCyte Antigen Retrieval Reagent-Basic

VCTS021
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

VisUCyte Antigen Retrieval Reagent-Acidic

VCTS022
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Control

Ctrl AB-108-C 191  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Secondary Antibodies
Description Application Cat# Citations Images  

Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG HRP-conjugated Antibody

WB HAF017 15  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 8
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG Antibody

WB AF109 6
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG VisUCyte HRP Polymer

IHC VC004  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Average Rating: 4 (Based on 2 reviews)

Have you used Human IL-17/IL-17A Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Read what people are saying who have used Human IL-17/IL-17A Antibody.

We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Immunohistochemistry.

Filter by Ratings
Very Good
(2)
All Ratings
Filter by Applications
IHC
(1)
All Applications
Filter by Species
Human
(1)
All Species

Images Ratings Applications Species Reviewed By Date Details
 Human IL‑17/IL‑17A Antibody AF-317-SP
Enlarge
Very Good
  Anonymous 04/21/2017
Show
Image Details
 Human IL‑17/IL‑17A Antibody AF-317-SP
: Human IL‑17/IL‑17A Antibody [AF-317-SP]

Other Experimental Details

Other Experimental DetailsAntigen retrieval pH 6.0 (20 min)Dilution-1:100, 1 h incubation.Detection with ImmPRESS HRP Anti-Goat Ig (Peroxidase) Polymer (# MP-7405-15 ) and TSA-Cy3 (#SAT704A001EA)
Immunohistochemistry Human IL-17/IL-17A Antibody AF-317-NA
Enlarge
Very Good
 IHC Human Anonymous 04/21/2017
Show
Image Details
Immunohistochemistry Human IL-17/IL-17A Antibody AF-317-NA
Immunohistochemistry: Human IL-17/IL-17A Antibody [AF-317-NA]

Summary

ApplicationImmunohistochemistry
Sample TestedSpleen tissue,Colon tissue
SpeciesHuman

Other Experimental Details

Other Experimental DetailsStaining is done in Leica Bond autostainer. Epitope retrieval at pH 6 (20 min), IL17/IL17A dilution 1:100, incubation time-1h. Detected with TSA-Cy3

Order Details

Contact Information

Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies

Feedback

Customer Information