Human IL-17F Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB13353-100
MAB13353-SP
Human IL‑17F ELISA Standard Curve.
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Human IL-17F Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-17F in direct ELISAs.
Source
Recombinant Monoclonal Mouse IgG2B Clone # 775602R
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IL-17F
Gly21-Thr153
Accession # Q96PD4
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human IL‑17F Monoclonal Antibody (Catalog # MAB13354).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human IL-17F DuoSet ELISA Kit (Catalog # DY1335B-05) for convenient development of a sandwich ELISA.

 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Human IL‑17F ELISA Standard Curve. Recombinant Human IL-17F protein was serially diluted 2-fold and captured by Mouse Anti-Human IL-17F Monoclonal Antibody (Catalog # MAB13354) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human IL-17F Monoclonal Antibody (Catalog # MAB13353) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-17F

The Interleukin 17 (IL‑17) family proteins, comprising six members (IL‑17A through IL‑17F), are secreted, structurally related proteins that share a conserved cystine‑knot fold near the C-terminus, but have considerable sequence divergence at the N‑terminus. With the exception of IL‑17B, which exists as a non‑covalently linked dimer, all IL-17 family members are disulfide-linked dimers. IL‑17 family proteins are pro-inflammatory cytokines that induce local cytokine production and are involved in the regulation of immune functions (1, 2).Human IL‑17F cDNA encodes a 163 aa protein with a putative 30 aa signal peptide. Among IL‑17 family members, IL‑17F is most closely related to IL-17A (approximately 44% aa sequence homology), but shares only limited sequence homology (16-30%) with IL‑17B, C, D and E. Human and mouse IL‑17F share 55% sequence identity. IL-17F is expressed in activated CD4+ T cells and activated monocytes. Five receptors (IL-17 RA, B, C, D and E) have been identified (5). Although the ligands for IL-17 RD and E are not known yet, it is reported that IL-17 RA binds IL-17A, and IL-17 RB binds
IL-17B and IL-17E. IL-17 RC binds IL-17A and IL‑17F with similarly high affinity and functions as a receptor for both IL-17A and IL-17F (5, 6). The biological activities mediated by IL‑17F are similar to those of IL‑17. IL‑17F stimulates production of IL‑6, IL‑8, G‑CSF, and regulates cartilage matrix turnover by increasing matrix release and inhibiting new matrix synthesis (4). IL‑17F also inhibits angiogenesis and induces production of IL‑2, TGF-beta, and monocyte chemoattractant protein‑1 in endothelial cells (3).

References
  1. Aggarwal, S. and A.L. Gurney (2002) J. Leukoc. Biol. 71:1.
  2. Moseley, T.A. et al. (2003) Cytokine & Growth Factor Rev. 14:155.
  3. Starnes, T. et al. (2001) J. Immunol. 167:4137.
  4. Shen, F. & S. L. Gaffen (2008) Cytokine 41:92.
  5. Kuestner, R.E. et al. (2007) J. Immunol. 179:5462.
Long Name
Interleukin 17F
Entrez Gene IDs
112744 (Human); 257630 (Mouse); 301291 (Rat); 708220 (Rhesus Macaque)
Alternate Names
Cytokine ML-1; IL17F; IL-17F; IL-17Finterleukin-17F; interleukin 17F

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