Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry

Cited:

Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 545503
View all LRP-1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human LRP-1
Gln4449-ala4544
Accession # Q07954

Specificity

Detects human LRP-1 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human LRP‑1 Beta Subunit Antibody

Detection of Human LRP-1 antibody by Western Blot.

Detection of Human LRP-1 by Western Blot.

Western blot shows lysates of human liver tissue. PVDF Membrane was probed with 2 µg/mL of Human LRP-1 beta subunit Monoclonal Antibody (Catalog # MAB6360) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for LRP-1 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
LRP-1 antibody in HepG2 Human Cell Line by Immunocytochemistry (ICC).

LRP‑1 in HepG2 Human Cell Line.

LRP-1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human LRP-1 beta subunit Monoclonal Antibody (Catalog # MAB6360) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
LRP-1 antibody in Human Liver by Immunohistochemistry (IHC-P).

LRP‑1 in Human Liver.

LRP-1 was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human LRP-1 beta subunit Monoclonal Antibody (Catalog # MAB6360) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in hepatocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human LRP‑1 Beta Subunit Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HepG2 human hepatocellular carcinoma cell line

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver

Western Blot

2 µg/mL
Sample: Human liver tissue under reducing conditions only

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LRP-1

LRP-1 (low-density lipoprotein receptor-related protein 1) is a large type I transmembrane protein belonging to the LDL receptor superfamily. The extracellular domain of LRP-1 is organized in four clusters of ligand-binding repeats that recognizes at least 30 different ligands. The amino acid sequence of human and mouse LRP-1 cluster II share 100% identity. LRP-1 is an endocytic receptor involved in endocytosis and in phagocytosis of apoptotic cells. It plays a crucial role in early embryonic development.

Long Name

LDL Receptor-related Protein 1

Alternate Names

A2MR, Alpha-2-Macroglobulin Receptor, APOER, Apolipoprotein E receptor, APR, CD91, CD91 antigen, EC 1.1.1.94, EC 3.4.21.9, IGFBP3R, low density lipoprotein receptor-related protein 1, LRP, LRP-1, LRP1A, prolow-density lipoprotein receptor-related protein 1, TbetaR-V/LRP-1/IGFBP-3 receptor, TGFBR5, type V tgf-beta receptor

Entrez Gene IDs

4035 (Human)

Gene Symbol

LRP1

UniProt

Q07954 (Human)

Additional LRP-1 Products

Product Documents for Human LRP‑1 Beta Subunit Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human LRP‑1 Beta Subunit Antibody

For research use only

Citations for Human LRP‑1 Beta Subunit Antibody

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Protocols

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FAQs

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