Human MMP-8 Antibody

Catalog # Availability Size / Price Qty
MAB908-SP
MAB908-100
MAB908-500
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews (2)

Human MMP-8 Antibody Summary

Species Reactivity
Human
Specificity
Detects the pro and active forms of human MMP-8 in Western blots. Does not cross-react with recombinant human (rh) MMP‑1, -2, -3, -7, -9,
-10, -12, or -13.
Source
Monoclonal Mouse IgG2B Clone # 100619
Purification
Protein A or G purified from ascites
Immunogen
Mouse myeloma cell line NS0-derived recombinant human MMP‑8
Phe21-Gly467
Accession # AAZ38714
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human MMP‑8 Western Blot Standard (Catalog # WBC017)
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human MMP‑8 (Catalog # 908-MP), see our available Western blot detection antibodies

Human MMP-8 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human MMP‑8 Biotinylated Antibody (Catalog # BAF908)

Standard: Recombinant Human MMP-8 Protein, CF (Catalog # 908-MP)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MMP-8

Matrix metalloproteinases (MMPs) are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP‑8 (neutrophil collagenase) is expressed in neutrophils, where it is stored in specific granules. MMP‑8 release from the neutrophils is stimulated by various factors such as interleukins 1 and 8, TNF-alpha and GM-CSF. MMP‑8 is capable of cleaving types I, II and III triple-helical collagen, gelatin peptides, fibronectin, proteoglycans, aggrecan, serpins, beta -casein and peptides such as angiotensin and substance P. In addition to its function in phagocytosis,
MMP‑8 has a high capacity for infiltrating connective tissue, and is implicated in the breakdown of the extracellular matrix in diseases such as rheumatoid arthritis. Structurally, MMP‑8 consists of several domains: a pro-domain that is cleaved upon activation, a catalytic domain containing the zinc-binding site, a short hinge region and a hemopexin-like domain. MMP‑8 is heavily glycosylated.

Long Name
Matrix Metalloproteinase 8
Entrez Gene IDs
4317 (Human); 17394 (Mouse); 63849 (Rat)
Alternate Names
CLG1HNC; Collagenase 2; EC 3.4.24; EC 3.4.24.34; matrix metallopeptidase 8 (neutrophil collagenase); matrix metalloproteinase 8 (neutrophil collagenase); Matrix metalloproteinase-8; MMP8; MMP-8; neutrophil collagenase; PMNL collagenase; PMNL-CL

Product Datasheets

Citations for Human MMP-8 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Loss of MMP-8 in ductal carcinoma in situ (DCIS)-associated myoepithelial cells contributes to tumour promotion through altered adhesive and proteolytic function
    Authors: M Sarper, MD Allen, J Gomm, L Haywood, J Decock, S Thirkettle, A Ustaoglu, SJ Sarker, J Marshall, DR Edwards, JL Jones
    Breast Cancer Res, 2017;19(1):33.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Matrix metalloprotease-9 dysregulation in lower airway secretions of cystic fibrosis patients.
    Authors: Gaggar A, Li Y, Weathington N, Winkler M, Kong M, Jackson P, Blalock JE, Clancy JP
    Am. J. Physiol. Lung Cell Mol. Physiol., 2007;293(1):L96-L104.
    Species: Human
    Sample Types: Sputum
    Applications: Activity Assay

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human MMP-8 Antibody

Average Rating: 3.5 (Based on 2 Reviews)

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Human MMP-8 Antibody
By Anonymous on 07/13/2018
Application: WB Sample Tested: HeLa cells Species: Mouse

Human MMP-8 Antibody
By Anonymous on 03/15/2018
Application: ELISA Sample Tested: EDTA Plasma Species: Human