Human/Mouse MEF2C Antibody

Catalog # Availability Size / Price Qty
MAB6786
MAB6786-SP
MEF2C in C2C12 Mouse Cell Line.
2 Images
Product Details
Citations (3)
FAQs
Supplemental Products
Reviews (3)

Human/Mouse MEF2C Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human MEF2C in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human SRF is observed.
Source
Monoclonal Mouse IgG2B Clone # 681824
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human MEF2C
Ala135-Lys239
Accession # Q06413
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
5-25 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry MEF2C antibody in C2C12 Mouse Cell Line by Immunocytochemistry (ICC). View Larger

MEF2C in C2C12 Mouse Cell Line. MEF2C was detected in immersion fixed differentiated C2C12 mouse myoblast cell line using Mouse Anti-Human/Mouse MEF2C Monoclonal Antibody (Catalog # MAB6786) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counter-stained with DAPI (blue). Specific staining was localized to nucleus of myoblasts. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry MEF2C antibody in Human Brain by Immunohistochemistry (IHC-P). View Larger

MEF2C in Human Brain. MEF2C was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Mouse Anti-Human/Mouse MEF2C Monoclonal Antibody (Catalog # MAB6786) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MEF2C

MEF2C (myocyte enhancer factor-2C) is a 51 kDa (predicted) protein that is one of four members of the MEF2/MADS (MCM1, Agamous, Deficiens, Serum response) box family of transcription factors. The 473 amino acid (aa) human MEF2C contains a MADS box for dimerization (aa 3-57), a DNA binding domain (aa 58-86), a beta domain (aa 271-278; missing in a 465 aa isoform), a transcriptional repressor (aa 368-399; missing in a 441 aa isoform) and several acetylation and ser/thr phosphorylation sites. Within aa 135-239, human MEF2C shares 95% and 98% aa identity with mouse and rat MEF2C, respectively. MEF2C plays important roles in preferential lymphoid differentiation of hematopoietic precursors, and in neuronal, cardiac and skeletal muscle morphogenesis.

Long Name
Myocyte-specific Enhancer Factor 2C
Entrez Gene IDs
4208 (Human); 17260 (Mouse); 499497 (Rat)
Alternate Names
DEL5q14.3; MEF2C; myocyte enhancer factor 2C; myocyte-specific enhancer factor 2C; polypeptide C

Product Datasheets

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Citations for Human/Mouse MEF2C Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Tau activation of microglial cGAS-IFN reduces MEF2C-mediated cognitive resilience
    Authors: JC Udeochu, S Amin, Y Huang, L Fan, ERS Torres, GK Carling, B Liu, H McGurran, G Coronas-Sa, G Kauwe, GA Mousa, MY Wong, P Ye, RK Nagiri, I Lo, J Holtzman, C Corona, A Yarahmady, MT Gill, RM Raju, SA Mok, S Gong, W Luo, M Zhao, TE Tracy, RR Ratan, LH Tsai, SC Sinha, L Gan
    Nature Neuroscience, 2023-04-24;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. MEF2 is a key regulator of cognitive potential and confers resilience to neurodegeneration
    Authors: Scarlett J. Barker, Ravikiran M. Raju, Noah E.P. Milman, Jose Davila-Velderrain, Fatima Davila-Velderrain, Fatima Gunter-Rahman et al.
    Science Translational Medicine
  3. Orai1 inhibitor STIM2 beta regulates myogenesis by controlling SOCE dependent transcriptional factors
    Authors: Kyu Min Kim, Anshul Rana, Chan Young Park
    Scientific Reports

FAQs

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Reviews for Human/Mouse MEF2C Antibody

Average Rating: 4.3 (Based on 3 Reviews)

5 Star
33.33%
4 Star
66.67%
3 Star
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Human/Mouse MEF2C Antibody
By Anonymous on 07/08/2022
Application: IHC Sample Tested: Brain tissue Species: Mouse

Human/Mouse MEF2C Antibody
By Anonymous on 12/06/2019
Application: MiAr Sample Tested: EDTA Plasma Species: Human

Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma


Human/Mouse MEF2C Antibody
By Anonymous on 11/10/2018
Application: ELISA Sample Tested: Plasma Species: Human