Human/Mouse MEF2C Antibody

Name: Human/Mouse MEF2C Antibody
Brand: R&D Systems
SKU: MAB6786-SP
Rating: 4.3 (3 reviews)

Catalog #: MAB6786 3 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6786
MAB6786-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse MEF2C Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human MEF2C in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human SRF is observed.

Source

Monoclonal Mouse IgG_2B Clone # 681824

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human MEF2C
Ala135-Lys239
Accession # Q06413

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

5-25 µg/mL

See below

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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MEF2C in C2C12 Mouse Cell Line. MEF2C was detected in immersion fixed differentiated C2C12 mouse myoblast cell line using Mouse Anti-Human/Mouse MEF2C Monoclonal Antibody (Catalog # MAB6786) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counter-stained with DAPI (blue). Specific staining was localized to nucleus of myoblasts. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry

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MEF2C in Human Brain. MEF2C was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Mouse Anti-Human/Mouse MEF2C Monoclonal Antibody (Catalog # MAB6786) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MEF2C

MEF2C (myocyte enhancer factor-2C) is a 51 kDa (predicted) protein that is one of four members of the MEF2/MADS (MCM1, Agamous, Deficiens, Serum response) box family of transcription factors. The 473 amino acid (aa) human MEF2C contains a MADS box for dimerization (aa 3-57), a DNA binding domain (aa 58-86), a beta domain (aa 271-278; missing in a 465 aa isoform), a transcriptional repressor (aa 368-399; missing in a 441 aa isoform) and several acetylation and ser/thr phosphorylation sites. Within aa 135-239, human MEF2C shares 95% and 98% aa identity with mouse and rat MEF2C, respectively. MEF2C plays important roles in preferential lymphoid differentiation of hematopoietic precursors, and in neuronal, cardiac and skeletal muscle morphogenesis.

Long Name

Myocyte-specific Enhancer Factor 2C

Entrez Gene IDs

4208 (Human); 17260 (Mouse); 499497 (Rat)

Alternate Names

DEL5q14.3; MEF2C; myocyte enhancer factor 2C; myocyte-specific enhancer factor 2C; polypeptide C