Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunocytochemistry

Cited:

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 681824
View all MEF2C Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human MEF2C
Ala135-Lys239
Accession # Q06413

Specificity

Detects human MEF2C in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human SRF is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human/Mouse MEF2C Antibody

MEF2C antibody in C2C12 Mouse Cell Line by Immunocytochemistry (ICC).

MEF2C in C2C12 Mouse Cell Line.

MEF2C was detected in immersion fixed differentiated C2C12 mouse myoblast cell line using Mouse Anti-Human/Mouse MEF2C Monoclonal Antibody (Catalog # MAB6786) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counter-stained with DAPI (blue). Specific staining was localized to nucleus of myoblasts. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

MEF2C antibody in Human Brain by Immunohistochemistry (IHC-P).

MEF2C in Human Brain.

MEF2C was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Mouse Anti-Human/Mouse MEF2C Monoclonal Antibody (Catalog # MAB6786) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human/Mouse MEF2C Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed differentiated C2C12 mouse myoblast cell line

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cortex)

Reviewed Applications

Read 3 reviews rated 4.3 using MAB6786 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MEF2C

MEF2C (myocyte enhancer factor-2C) is a 51 kDa (predicted) protein that is one of four members of the MEF2/MADS (MCM1, Agamous, Deficiens, Serum response) box family of transcription factors. The 473 amino acid (aa) human MEF2C contains a MADS box for dimerization (aa 3-57), a DNA binding domain (aa 58-86), a beta domain (aa 271-278; missing in a 465 aa isoform), a transcriptional repressor (aa 368-399; missing in a 441 aa isoform) and several acetylation and ser/thr phosphorylation sites. Within aa 135-239, human MEF2C shares 95% and 98% aa identity with mouse and rat MEF2C, respectively. MEF2C plays important roles in preferential lymphoid differentiation of hematopoietic precursors, and in neuronal, cardiac and skeletal muscle morphogenesis.

Long Name

Myocyte-specific Enhancer Factor 2C

Alternate Names

DEL5q14.3

Entrez Gene IDs

4208 (Human); 17260 (Mouse); 499497 (Rat)

Gene Symbol

MEF2C

UniProt

Q06413

Additional MEF2C Products

Product Documents for Human/Mouse MEF2C Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse MEF2C Antibody

For research use only

Citations for Human/Mouse MEF2C Antibody

Customer Reviews for Human/Mouse MEF2C Antibody (3)

4.3 out of 5
3 Customer Ratings
5 Stars
33%
4 Stars
67%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • Human/Mouse MEF2C Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Brain tissue
    Species: Mouse
    Verified Customer | Posted 07/08/2022
    Human/Mouse MEF2C Antibody MAB6786
  • Human/Mouse MEF2C Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 12/06/2019
    Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma
    Human/Mouse MEF2C Antibody MAB6786
  • Human/Mouse MEF2C Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Plasma
    Species: Human
    Verified Customer | Posted 11/10/2018

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Protocols

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