Human/Mouse/Rat Akt1 Antibody

Catalog # Availability Size / Price Qty
MAB1775
MAB1775-SP

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Detection of Human Akt1 by Western Blot.
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Human/Mouse/Rat Akt1 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat Akt1 in Western blots. In Western blots, no cross-reactivity with recombinant human Akt2 or Akt3 is observed.
Source
Monoclonal Mouse IgG2A Clone # 232790
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Akt1
Ser2-Ala480
Accession # P31749
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.2 µg/mL
See below
Knockout Validated
Akt1 is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in Akt1 knockout HeLa cell line.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human Akt1 antibody by Western Blot. View Larger

Detection of Human Akt1 by Western Blot. Western blot shows recombinant human Akt1, Akt2, and Akt3 (5 ng/lane). PVDF membrane was probed with 0.2 µg/mL Mouse Anti-Human/Mouse/Rat Akt1 Monoclonal Antibody (Catalog # MAB1775) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for Akt1 was detected at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Western Blot Detection of Human, Mouse, and Rat Akt1 antibody by Western Blot. View Larger

Detection of Human, Mouse, and Rat Akt1 by Western Blot. Western blot shows lysates of MBA-MB-123 human breast cancer cell line, C2C12 mouse myoblast cell line, TS1 mouse helper T cell line, and C6 rat glioma cell line. PVDF membrane was probed with 0.2 µg/mL Mouse Anti-Human/Mouse/Rat Akt1 Monoclonal Antibody (Catalog # MAB1775) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for Akt1 was detected at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Knockout Validated Western Blot Shows Human Akt1 Antibody Specificity by Using Knockout Cell Line. View Larger

Western Blot Shows Human Akt1 Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Atk1 knockout HeLa cell line (KO). PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human/Mouse/Rat Akt1 Monoclonal Antibody (Catalog # MAB1775) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Akt1 at approximately 72 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Akt1

Akt, also known as protein kinase B (PKB), is a central kinase in such diverse cellular processes as glucose uptake, cell cycle progression, and apoptosis. Three highly homologous members define the Akt family: Akt1 (PKB alpha ), Akt2 (PKB beta ), and Akt3 (PKB gamma ). Akt1 is the most ubiquitously expressed family member. All three Akts contain an amino-terminal pleckstrin homology domain, a central kinase domain, and a carboxyl-terminal regulatory domain.

Long Name
v-Akt Murine Thymoma Viral Oncogene Homolog 1
Entrez Gene IDs
207 (Human); 11651 (Mouse); 25233 (Rat)
Alternate Names
AKT serine/threonine kinase 1; AKT; Akt1; AKT1m; CWS6; EC 2.7.11; EC 2.7.11.1; PKB alpha; PKB; PKBMGC99656; PRKBA; Protein Kinase B Alpha; Protein kinase B; Proto-oncogene c-Akt; rac protein kinase alpha; RAC; RAC-alpha serine/threonine-protein kinase; RAC-alpha; RAC-PK-alpha; RACPKB-ALPHA; Serine-Threonine Protein Kinase ; v-akt murine thymoma viral oncogene homolog 1; V-Akt Murine Thymoma Viral Oncogene-Like Protein 1

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Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

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