Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 336016
View all B-Raf Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human B-Raf
Met1-His766
Accession # P15056

Specificity

Detects human, mouse, and rat B-Raf. No cross-reactivity with recombinant human Raf-1 is observed. Cross-reactivity with A-Raf has not been tested.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human/Mouse/Rat B‑Raf Antibody

Detection of Human/Mouse/Rat B‑Raf antibody by Western Blot.

Detection of Human/Mouse/Rat B‑Raf by Western Blot.

Western blot shows lysates of MDA-MB-468 human breast cancer cell line, Raji human Burkitt's lymphoma cell line, and Ramos human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL Mouse Anti-Human/Mouse/Rat B-Raf Monoclonal Antibody (Catalog # MAB3424) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, Recombinant Human Active Raf-1 (Y340E Y341E), aa 306-648 (Catalog # 4540-KS) and recombinant human B-Raf (5 ng/lane) were included. A specific band for B-Raf was detected at approximately 95 kDa (as indicated). An unidentified band at 55 kDa was also detected. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Applications for Human/Mouse/Rat B‑Raf Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: MDA-MB-468 human breast cancer cell line, Raji human Burkitt's lymphoma cell line, and Ramos human Burkitt's lymphoma cell line

Reviewed Applications

Read 1 review rated 1 using MAB3424 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: B-Raf

The Raf serine/threonine kinases are effectors of Ras that function as MAP3Ks in the ERK phosphorylation cascade. Mammals express three Raf proteins: Raf-1 (C‑Raf); A-Raf; and B-Raf, strongly expressed in fetal brain and adult cerebrum. Mice with a targeted disruption of the B-raf gene die of vascular defects during mid‑gestation. B-raf mutations have been found in two-thirds of malignant melanomas, with the single substitution V599E in the kinase domain the most frequent occurrence.

Long Name

v-Raf Murine Sarcoma Viral Oncogene Homolog B1

Alternate Names

BRaf, BRAF1, RAFB1

Entrez Gene IDs

673 (Human); 114486 (Rat)

Gene Symbol

BRAF

UniProt

P15056

Additional B-Raf Products

Product Documents for Human/Mouse/Rat B‑Raf Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human/Mouse/Rat B‑Raf Antibody

For research use only

Related Research Areas

Cancer Biomarkers
Intracellular Kinases

Customer Reviews for Human/Mouse/Rat B‑Raf Antibody (1)

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  • Human/Mouse/Rat B-Raf Antibody
    Name: Dwayne Stupack
    Application: Western Blot
    Sample Tested: Cell Lysates
    Species: Mouse and Human
    Verified Customer | Posted 02/24/2023
    Blotted in parallel with MAB4550 (anti-CRAF), which gives bands. (second lane is a CRAF-nul cell line, though).
    Human/Mouse/Rat B‑Raf Antibody MAB3424
    Bio-Techne Response
    Thank you for reviewing our product. We are sorry to hear that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.

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