Human/Mouse/Rat FPRP Antibody

Catalog # Availability Size / Price Qty
MAB10043-100
MAB10043-SP
Detection  of Human, Mouse, and Rat FPRP by Western Blot.
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Human/Mouse/Rat FPRP Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human FPRP in direct ELISAs. Detects human, mouse, and rat FPRP in Western blots.
Source
Monoclonal Mouse IgG2B Clone # 998175
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human embryonic kidney cell, HEK293-derived human FPRP
Pro27-Pro832
Accession # Q9P2B2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Immunohistochemistry
5-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection  of Human, Mouse, and Rat FPRP by Western Blot. View Larger

Detection of Human, Mouse, and Rat FPRP by Western Blot. Western blot shows lysates of LoVo human colorectal adenocarcinoma cell line, NIH‑3T3 mouse embryonic fibroblast cell line, C2C12 mouse myoblast cell line, and PC‑12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse/Rat FPRP Monoclonal Antibody (Catalog # MAB10043) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for FPRP at approximately 135 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry FPRP in  Human Lung Cancer Tissue. View Larger

FPRP in Human Lung Cancer Tissue. FPRP was detected in immersion fixed paraffin-embedded sections of human lung cancer tissue using Mouse Anti-Human FPRP Monoclonal Antibody (Catalog # MAB10043) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surfaces in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FPRP

FPRP (F2 alpha Prostaglandin receptor Regulatory Protein; also CD315, EWI-F and CD9 partner-1/P-1) is a 130 kDa type I transmembrane (TM) glycoprotein member of the EWI subfamily, Ig superfamily of molecules. It associates with the 7-TM prostaglandin F2 alpha  receptor, inhibiting its activity. It also forms complexes with 4-TM CD9 and CD81, linking these molecules to the intracellular actin cytoskeleton.

Long Name
Prostaglandin F2-alpha Receptor Regulatory Protein
Entrez Gene IDs
5738 (Human); 19221 (Mouse); 29602 (Rat)
Alternate Names
CD315; CD9P-1; FPRP; PTGFRN

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Mouse IgG2B Antibody

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