Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 1032149
View all MCHR1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived human MCHR1
Met1-Pro113
Accession # Q99705

Specificity

Detects human MCHR1 in direct ELISAs. Detects human, mouse, and rat MCHR1 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human/Mouse/Rat MCHR1 Antibody

Detection of Human, Mouse, and Rat MCHR1 by Western Blot.

Western blot shows lysates of human hypothalamus tissue, mouse hypothalamus tissue, and rat hypothalamus tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat MCHR1 Monoclonal Antibody (Catalog # MAB79381) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for MCHR1 at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

MCHR1 in Human Brain (Hypothalamus).

MCHR1 was detected in immersion fixed paraffin-embedded sections of human brain (hypothalamus) using Mouse Anti-Human/Mouse/Rat MCHR1 Monoclonal Antibody (Catalog # MAB79381) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human/Mouse/Rat MCHR1 Antibody

Application
Recommended Usage

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (hypothalamus) 

Western Blot

1 µg/mL
Sample: Human hypothalamus tissue, Mouse hypothalamus tissue, and Rat hypothalamus tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MCHR1

MCHR1 (Melanin-concentrating Hormone Receptor-1), also called GPR24 (G-protein Coupled Receptor 24) or SLC1 (Somatostatin Receptor-like 1), is an approximately 44 kDa, seven-transmembrane receptor for MHC. It is most highly expressed in the brain but is also present in peripheral tissues. Engagement of MHCR1 has effects on food intake, energy homeostasis, anxiety and depression, such that rodents treated with small molecule antagonists weigh less and show reduced anxiety and depression in model systems than untreated rodents. Human MCHR1 shares 74% amino acid (aa) sequence identity with mouse MCHR1 between aa 1-113, and 82% with rat MCHR1 between aa 71-113.

Long Name

Melanin-concentrating Hormone Receptor 1

Alternate Names

GPR24, MCH1R, SLC1

Entrez Gene IDs

2847 (Human); 207911 (Mouse); 83567 (Rat)

Gene Symbol

MCHR1

UniProt

Q99705 (Human)

Additional MCHR1 Products

Product Documents for Human/Mouse/Rat MCHR1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat MCHR1 Antibody

For research use only

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Protocols

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