Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 1034083
View all OX40 Ligand/TNFSF4 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived human OX40 Ligand/TNFRSF4
Gln51-Leu183
Accession # Q6FGS4

Specificity

Detects human OX40 Ligand/TNFRSF4 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human OX40 Ligand/TNFSF4 Antibody

OX40 Ligand/TNFRSF4 in Human Testis.

OX40 Ligand/TNFRSF4 was detected in immersion fixed paraffin-embedded sections of human testis using Mouse Anti-Human OX40 Ligand/TNFRSF4 Monoclonal Antibody (Catalog # MAB10656) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface in sperm cells. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human OX40 Ligand/TNFSF4 Antibody

Application
Recommended Usage

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human testis

Reviewed Applications

Read 1 review rated 5 using MAB10656 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: OX40 Ligand/TNFSF4

OX40 Ligand (OX40L), also known as gp34, is a type II transmembrane glycoprotein belonging to the TNF superfamily. Human OX40L cDNA encodes a 183 amino acids (aa) polypeptide with an amino-terminal cytoplasmic domain (aa 1¬23) and a carboxy¬terminal extracellular domain (aa 51-183). It shares 46% aa sequence identity with the mouse counterpart. OX40L is expressed on the surface of activated B cells, T cells, dendritic cells and endothelial cells. Similarly to other TNF superfamily members, membrane-bound OX40 Ligand exists as a homotrimer. OX40L binds to OX40 (CD134), a member of the TNF receptor superfamily that is expressed predominantly on activated CD4+ T cells. OX40 Ligand is one of the co¬stimulatory molecules in the immune system that includes B7, CD40 Ligand, CD30 Ligand, CD27 Ligand and 4¬1BB Ligand. Because OX40 appears as a late activation-induced T cell surface antigen, it has been speculated that the major function of OX40-OX40L interaction is to transmit a late co-stimulatory signal to promote the survival and proliferation of activated CD4+ T cells and prolong the immune response. Engagement of OX40 on activated T cells in situ in tumors has been shown to augment immune responses and subsequent tumor regression.

References

  1. Godfrey, W.R. et al. (1994) J. Exp. Med. 180:757
  2. Baum, P.R. et al. (1994) EMBO J. 13:3992.
  3. Al­Shamkhani, A. et al. (1997) J. Biol. Chem. 272:5275.
  4. Kjaergaard, J. et al. (2000) Cancer Res. 60:5514.

Alternate Names

CD252, gp34, TNFSF4

Entrez Gene IDs

7292 (Human); 22164 (Mouse); 89814 (Rat)

Gene Symbol

TNFSF4

UniProt

Q6FGS4

Additional OX40 Ligand/TNFSF4 Products

Product Documents for Human OX40 Ligand/TNFSF4 Antibody

Certificate of Analysis

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Product Specific Notices for Human OX40 Ligand/TNFSF4 Antibody

For research use only

Customer Reviews for Human OX40 Ligand/TNFSF4 Antibody (1)

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  • Human OX40/TNFRSF4 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Testis tissue
    Species: Human
    Verified Customer | Posted 03/22/2022
    Human OX40 Ligand/TNFSF4 Antibody MAB10656

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