Human Phospho-Akt1 (T308) Antibody

(1 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human and mouse Akt1 when phosphorylated at T308.
  • Source
    Monoclonal Mouse IgG3 Clone # 658320
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Phosphopeptide containing the human Akt1 T308 site
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in Tris with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human and Mouse Phospho-Akt1 (T308) by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and NIH‑3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human PDGF‑BB (Catalog # 220-BB) for 20 minutes or 100nM Calyculin A for 30 minutes. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Phospho-Akt1 (T308) Monoclonal Antibody (Catalog # MAB7419) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Phospho-Akt1 (T308) at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
Phospho-Akt1 (T308) in CCD‑1070Sk Human Cell Line. Akt1 phosphorylated at T308 was detected in immersion fixed CCD‑1070Sk human foreskin fibroblast cell line stimulated with Recombinant Human PDGF‑BB (Catalog # 220-BB) using Mouse Anti-Human Phospho-Akt1 (T308) Mono­clonal Antibody (Catalog # MAB7419) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the Northern­Lights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Akt1
Akt, also known as protein kinase B (PKB), is a central kinase in such diverse cellular processes as glucose uptake, cell cycle progression, and apoptosis. Three highly homologous members define the Akt family: Akt1 (PKB alpha ), Akt2 (PKB beta ), and Akt3 (PKB gamma ). All three Akts contain an amino-terminal pleckstrin homology domain, a central kinase domain, and a carboxyl-terminal regulatory domain. Akt1 is the most widely expressed family member and is frequently activated in a number of carcinomas, including breast, prostate, lung, pancreatic, liver, ovarian, and colorectal cancer. Akt1 is activated in a multistep process that involves the sequential phosphorylation of Thr450 by JNK kinases, Thr308 by PDK1, and Ser473 by PDK2 or mTORC2. Activated Akt1 phosphorylates a wide variety of cytosolic, nuclear, and mitochondrial substrates. Human Akt1 shares 98% aa sequence identity with mouse and rat Akt1. MAB7419 also detects mouse Phospho-Akt1 (T308) in Western Blot.
  • Long Name:
    v-Akt Murine Thymoma Viral Oncogene Homolog 1
  • Entrez Gene IDs:
    207 (Human); 11651 (Mouse); 25233 (Rat)
  • Alternate Names:
    AKT; Akt1; EC 2.7.11; EC 2.7.11.1; PKB alpha; PKBMGC99656; PRKBA; Protein kinase B; Proto-oncogene c-Akt; rac protein kinase alpha; RAC-alpha serine/threonine-protein kinase; RAC-alpha; RAC-PK-alpha; RACPKB-ALPHA; v-akt murine thymoma viral oncogene homolog 1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. ASPP2 Is a Novel Pan-Ras Nanocluster Scaffold
    PLoS ONE, 2016;11(7):e0159677.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
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