Human PI 3-Kinase p110 delta Antibody

Catalog # Availability Size / Price Qty
MAB2687
MAB2687-SP
Human PI 3-Kinase p110 delta Antibody in Data
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Product Details
Citations (2)
FAQs
Supplemental Products
Reviews (4)

Human PI 3-Kinase p110 delta Antibody Summary

Species Reactivity
Human
Specificity
Detects endogenous human PI 3-kinase p110δ in Western blots.
Source
Monoclonal Mouse IgG2B Clone # 275144
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human PI 3-Kinase p110δ
Cys391-Phe542
Accession # O00329
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human PI 3-Kinase p110d antibody by Western Blot. View Larger

Detection of Human PI 3-Kinase p110δ by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line, Jurkat human acute T cell leukemia cell line, and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human PI 3-Kinase p110d Monoclonal Antibody (Catalog # MAB2687) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PI 3-Kinase p110 delta at approximately 110 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PI 3-Kinase p110 delta

Class IA phosphoinositide 3-kinases (PI 3-kinases) are a family of p85/p110 heterodimeric lipid kinases that function as signal transducers downstream of growth factor receptors, mainly receptor tyrosine kinases. Mammals express three class IA catalytic subunits: p110 alpha, p110 beta, and p110δ. The p110δ subunit is expressed predominantly in leukocytes, indicating that it may play a role in immune signaling.

Long Name
Protein Tyrosine Phosphatase 1D
Entrez Gene IDs
5293 (Human)
Alternate Names
EC 2.7.1; EC 2.7.1.153; p110D; P110DELTA; Phosphatidylinositol-4,5-bisphosphate 3-kinase 110 kDa catalytic subunit delta; phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta isoform; phosphoinositide-3-kinase C; phosphoinositide-3-kinase, catalytic, delta polypeptide; PI 3Kinase p110 delta; PI 3-Kinase p110 delta; PI3K; PI3K-delta; PI3-kinase p110 subunit delta; PI3-kinase subunit delta; PIK3CD; PtdIns-3-kinase subunit delta; PtdIns-3-kinase subunit p110-delta

Product Datasheets

Citations for Human PI 3-Kinase p110 delta Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. PI3K? and PI3K? isoforms have distinct functions in regulating pro-tumoural signalling in the multiple myeloma microenvironment
    Authors: RE Piddock, N Loughran, CR Marlein, SD Robinson, DR Edwards, S Yu, GE Pillinger, Z Zhou, L Zaitseva, MJ Auger, SA Rushworth, KM Bowles
    Blood Cancer J, 2017;7(3):e539.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Identification of HSP90 inhibitors as a novel class of senolytics
    Authors: H Fuhrmann-S, YY Ling, J Zhao, SJ McGowan, Y Zhu, RW Brooks, D Grassi, SQ Gregg, JL Stripay, A Dorronsoro, L Corbo, P Tang, C Bukata, N Ring, M Giacca, X Li, T Tchkonia, JL Kirkland, LJ Niedernhof, PD Robbins
    Nat Commun, 2017;8(1):422.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human PI 3-Kinase p110 delta Antibody

Average Rating: 4.3 (Based on 4 Reviews)

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Human PI 3-Kinase p110 delta Antibody
By Anonymous on 01/14/2021
Application: Microarrays Sample Tested: EDTA Plasma Species: Human

Human PI 3-Kinase p110 delta Antibody
By Anonymous on 02/07/2020
Application: MiAr Sample Tested: EDTA Plasma Species: Human

Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma


Human PI 3-Kinase p110d Antibody
By Anonymous on 11/14/2018
Application: Microarrays Sample Tested: EDTA Plasma Species: Human

Human PI 3-Kinase p110d Antibody
By Anonymous on 11/07/2018
Application: ELISA Sample Tested: Serum,Plasma Species: Human,Mouse