Human PI 3-Kinase p110 delta Antibody (275144)

R&D Systems | Catalog # MAB2687

R&D Systems

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 275144
View all PI 3-Kinase p110 delta Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PI 3-Kinase p110 delta
Cys391-Phe542
Accession # O00329

Specificity

Detects endogenous human PI 3-kinase p110 delta in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human PI 3-Kinase p110 delta Antibody (275144)

Detection of Human PI 3-Kinase p110d antibody by Western Blot.

Detection of Human PI 3-Kinase p110 delta by Western Blot.

Western blot shows lysates of Raji human Burkitt's lymphoma cell line, Jurkat human acute T cell leukemia cell line, and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human PI 3-Kinase p110d Monoclonal Antibody (Catalog # MAB2687) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PI 3-Kinase p110 delta at approximately 110 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.
Detection of Human PI 3-Kinase p110 delta by Western Blot

Detection of Human PI 3-Kinase p110 delta by Western Blot

Inhibition of PI3K delta and PI3K gamma subunits in MM proliferation. (a) Western blot analysis of PI3K delta and PI3K gamma subunits in MM cell lines and MM primary cells. Blots were probed with beta -actin to show sample loading. (b) MM primary cells were treated with idelalisib (1 μm), CZC24832 (1 μm) and duvelisib (1 μm) for 72 h and then assessed for cell viability using CellTiter Glo. (c) MM primary cells were treated with idelalisib (1 μm), CZC24832 (1 μm) and duvelisib (1 μm) for 72 h and then assessed for apoptosis by PI/Annexin V staining (n=4). Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/bcj201716), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human PI 3-Kinase p110 delta Antibody (275144)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Raji human Burkitt's lymphoma cell line, Jurkat human acute T cell leukemia cell line, and K562 human chronic myelogenous leukemia cell line

Reviewed Applications

Read 4 reviews rated 4.3 using MAB2687 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PI 3-Kinase p110 delta

Class IA phosphoinositide 3-kinases (PI 3-kinases) are a family of p85/p110 heterodimeric lipid kinases that function as signal transducers downstream of growth factor receptors, mainly receptor tyrosine kinases. Mammals express three class IA catalytic subunits: p110 alpha, p110 beta, and p110 delta. The p110 delta subunit is expressed predominantly in leukocytes, indicating that it may play a role in immune signaling.

Long Name

Protein Tyrosine Phosphatase 1D

Alternate Names

PI 3Kinase p110 delta, PIK3CD

Entrez Gene IDs

5293 (Human)

Gene Symbol

PIK3CD

UniProt

O00329

Additional PI 3-Kinase p110 delta Products

Product Documents for Human PI 3-Kinase p110 delta Antibody (275144)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human PI 3-Kinase p110 delta Antibody (275144)

For research use only

Citations for Human PI 3-Kinase p110 delta Antibody (275144)

Customer Reviews for Human PI 3-Kinase p110 delta Antibody (275144) (4)

4.3 out of 5
4 Customer Ratings
5 Stars
25%
4 Stars
75%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 4 of 4 reviews Showing All
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  • Human PI 3-Kinase p110 delta Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 01/14/2021
    Human PI 3-Kinase p110 delta Antibody (275144) MAB2687
  • Human PI 3-Kinase p110 delta Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 02/07/2020
    Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma
    Human PI 3-Kinase p110 delta Antibody (275144) MAB2687
  • Human PI 3-Kinase p110 delta Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 11/14/2018
  • Human PI 3-Kinase p110 delta Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human and Mouse
    Verified Customer | Posted 11/07/2018

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