Detects human VEGF165 and human VEGF121 in direct ELISAs and Western blots. In ELISAs, this antibody shows approximately 10% cross-reactivity with recombinant mouse (rm) VEGF and rrVEGF and no cross-reactivity with rhVEGF-D.
Recombinant Monoclonal Mouse IgG2B Clone # 26503R
Protein A or G purified from cell culture supernatant
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human VEGF165 Ala27-Arg191 Accession # NP_001165097
as a solution in PBS. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Recombinant Human VEGF 165 Protein (Catalog # 293-VE)
Measured by its ability to neutralize VEGF165-induced proliferation in HUVEC human umbilical vein endothelial cells. The Neutralization Dose (ND50) is typically 10-60 ng/mL in the presence of 10 ng/mL Recombinant Human VEGF165.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human VEGF by Western Blot. Western blot shows lysates of human lung tissue. PVDF membrane was probed with 2 µg/mL of Recombinant Mouse Anti-Human/Primate VEGF Monoclonal Antibody (Catalog # MAB293R) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for VEGF at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
VEGF in Human Liver. VEGF was detected in immersion fixed paraffin-embedded sections of human liver using Recombinant Mouse Anti-Human/Primate VEGF Monoclonal Antibody (Catalog # MAB293R) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Cell Proliferation Induced by VEGF165 and Neutralization by Human VEGF Antibody. Recombinant Human VEGF165 (Catalog # 293-VE) stimulates proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human VEGF165 (10 ng/mL) is neutralized (green line) by increasing concentrations of Recombinant Mouse Anti-Human/Primate VEGF Monoclonal Antibody (Catalog # MAB293R). The ND50 is typically 10-60 ng/mL.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C, as supplied.
1 month, 2 to 8 °C under sterile conditions after opening.
6 months, -20 to -70 °C under sterile conditions after opening.
VEGF is a soluble protein secreted by a wide variety of cell types. It
binds to the receptor tyrosine kinases VEGF R1 (Flt-1) and VEGF R2
(Flk-1). VEGF stimulates vascular endothelial cell proliferation and is
a potent inducer of angiogenesis. Several VEGF isoforms occur resulting
from alternative mRNA splicing.
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