Human/Primate VEGF Antibody Summary
10% cross-reactivity with recombinant mouse (rm) VEGF and rrVEGF and no cross-reactivity with rhVEGF-D.
Accession # NP_001165097
Human/Primate VEGF Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human VEGF by Western Blot. Western blot shows lysates of human lung tissue. PVDF membrane was probed with 2 µg/mL of Recombinant Mouse Anti-Human/Primate VEGF Monoclonal Antibody (Catalog # MAB293R) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for VEGF at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
VEGF in Human Liver. VEGF was detected in immersion fixed paraffin-embedded sections of human liver using Recombinant Mouse Anti-Human/Primate VEGF Monoclonal Antibody (Catalog # MAB293R) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Cell Proliferation Induced by VEGF165 and Neutralization by Human VEGF Antibody.
Recombinant Human VEGF165 (Catalog # 293-VE) stimulates proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human VEGF165 (10 ng/mL) is neutralized (green line) by increasing concentrations of Recombinant Mouse Anti-Human/Primate VEGF Monoclonal Antibody (Catalog # MAB293R). The ND50 is typically
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
VEGF is a soluble protein secreted by a wide variety of cell types. It binds to the receptor tyrosine kinases VEGF R1 (Flt-1) and VEGF R2 (Flk-1). VEGF stimulates vascular endothelial cell proliferation and is a potent inducer of angiogenesis. Several VEGF isoforms occur resulting from alternative mRNA splicing.
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Cell and Tissue Staining Kits
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