Human PTGER1 Antibody

Catalog # Availability Size / Price Qty
MAB100081-100
MAB100081-SP
Human PTGER1 Antibody in Immunohistochemistry
1 Image
Product Details
FAQs
Supplemental Products
Reviews

Human PTGER1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human PTGER1 in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 1014918
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Synthetic peptide containing human PTGER1
Accession # P34995
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
5-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry PTGER1  in Human Breast Cancer Tissue. View Larger

PTGER1 in Human Breast Cancer Tissue. PTGER1 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human PTGER1 Monoclonal Antibody (Catalog # MAB100081) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PTGER1

PTGER1 is a one of four EP1 receptors for PGE2 which is mediated by G(q) proteins.  PTGER1 may play a role as an important modulator of renal functions.  It has also been detected in mast cells, pulmonary veins, keratinocytes, myometrium and colon smooth muscle. EP1 expression has also frequently been seen in human breast cancers.  Nuclear expression of EP1 correlates with good prognosis markers like node negative status and PR expression. Animal model studies have implicated EP1 in various physiological and pathological responses however expression in humans appears to be more limited. EP1 receptor antagonists have been studied clinically primarily to treat hyperalgesia.

Long Name
Prostaglandin E2 Receptor 1, Subtype EP1
Entrez Gene IDs
5731 (Human); 19216 (Mouse); 25637 (Rat)
Alternate Names
EP1; PGE receptor EP1 subtype; PGE receptor, EP1 subtype; PGE2 receptor EP1 subtype; prostaglandin E receptor 1 (subtype EP1), 42kD; prostaglandin E receptor 1 (subtype EP1), 42kDa; prostaglandin E receptor 1, subtype EP1; prostaglandin E2 receptor EP1 subtype; Prostanoid EP1 receptor; PTGER1

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human PTGER1 Antibody

There are currently no reviews for this product. Be the first to review Human PTGER1 Antibody and earn rewards!

Have you used Human PTGER1 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review