Human TNF RII/TNFRSF1B Antibody
Human TNF RII/TNFRSF1B Antibody Summary
Leu23-Asp257
Accession # P20333
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of TNF RII/TNFRSF1B in Human Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with Mouse Anti-Human TNF RII/TNFRSF1B Monoclonal Antibody (Catalog # MAB226, filled histogram) or isotype control antibody (MAB003, open histogram) followed by anti-mouse IgG PE-conjugated secondary antibody (F0101B). View our protocol for Staining Membrane-associated Proteins.
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TNF RII/TNFRSF1B in Human PBMCs. TNF RII/TNFRSF1B was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human TNF RII/TNFRSF1B Monoclonal Antibody (Catalog # MAB226) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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TNF RII/TNFRSF1B Inhibition of TNF‑ alpha -induced Cytotoxicity and Neutralization by Human TNF RII/TNFRSF1B Antibody. Recombinant Human TNF RII/TNFRSF1B inhibits Recombinant Human TNF-a (210-TA) induced cytotoxicity in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of Recombinant Human TNF-a (0.25 ng/mL) activity elicited by Recombinant Human TNF RII/ TNFRSF1B (0.3 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human TNF RII/ TNFRSF1B Monoclonal Antibody (Catalog # MAB226). The ND50 is typically 0.5-1.5 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).
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Detection of TNF RII/TNFRSF1B in U937 cells by Flow Cytometry. U937 cells were stained with Mouse Anti-Human TNF RII/TNFRSF1B Monoclonal Antibody (Catalog # MAB226, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgM Secondary Antibody (Catalog # F0116). View our protocol for Staining Membrane-associated Proteins.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TNF RII/TNFRSF1B
TNF RII, also known as p75 or p80 TNF R, is a prototypic member of the TNF receptor superfamily and has been designated TNFRSF1B. It binds TNF with highaffinity and is expressed primarily in cells of the immune system.
Product Datasheets
Citations for Human TNF RII/TNFRSF1B Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
9
Citations: Showing 1 - 9
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Co-expression of TNF receptors 1 and 2 on melanomas facilitates soluble TNF-induced resistance to MAPK pathway inhibitors
Authors: CA Sander, EA Rush, J Shi, LMRB Arantes, RJ Tesi, MA Ross, MJ Calderon, SC Watkins, JM Kirkwood, RL Ferris, LH Butterfiel, L Vujanovic
Journal of Translational Medicine, 2022;20(1):331.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Low doses of LPS exacerbate the inflammatory response and trigger death on TLR3-primed human monocytes
Authors: M Monguió-To, M Franquesa, MR Sarrias, FE Borràs
Cell Death Dis, 2018;9(5):499.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Circulating TNF receptors 1 and 2 are associated with the severity of renal interstitial fibrosis in IgA nephropathy.
Authors: Sonoda Y, Gohda T, Suzuki Y, Omote K, Ishizaka M, Matsuoka J, Tomino Y
PLoS ONE, 2015;10(4):e0122212.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Tumor necrosis factor stimulates osteoclastogenesis from human bone marrow cells under hypoxic conditions.
Authors: Nomura T, Aoyama M, Waguri-Nagaya Y, Goto Y, Suzuki M, Miyazawa K, Asai K, Goto S
Exp Cell Res, 2014;321(2):167-77.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
FOXO3a regulates oxygen-responsive expression of tumor necrosis factor receptor 2 in human dermal microvascular endothelial cells.
Authors: Ding B, Kirkiles-Smith NC, Pober JS
J. Biol. Chem., 2009;284(29):19331-9.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Cutting edge: TNFR-shedding by CD4+CD25+ regulatory T cells inhibits the induction of inflammatory mediators.
Authors: van Mierlo GJ, Scherer HU, Hameetman M, Morgan ME, Flierman R, Huizinga TW, Toes RE
J. Immunol., 2008;180(5):2747-51.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Stent implantation in coronary porcine arteries is associated with early activation of TNFalpha and TNFalpha receptor II expression.
Authors: Hadjadj S, Cloutier I, Geoffroy P, Tanguay JF
Atherosclerosis, 2007;192(1):25-32.
Species: Porcine
Sample Types: Whole Tissue
Applications: IHC-P -
Human intestinal intraepithelial lymphocytes keep TNF alpha levels low by cell uptake and feedback inhibition of transcription.
Authors: Ebert EC, Mehta V
Cell. Immunol., 2006;241(1):7-13.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Synergistic induction of apoptosis in breast cancer cells by cotreatment with butyrate and TNF-alpha, TRAIL, or anti-Fas agonist antibody involves enhancement of death receptors' signaling and requires P21(waf1).
Authors: Chopin V, Slomianny C, Hondermarck H, Le Bourhis X
Exp. Cell Res., 2004;298(2):560-73.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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